Population Structure of Mixed Mycobacterium tuberculosis Infection Is Strain Genotype and Culture Medium Dependent

 

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dc.contributor.author Hanekom, Madeleine en_ZA
dc.contributor.author Streicher, Elizabeth M en_ZA
dc.contributor.author Van de Berg, Doreen en_ZA
dc.contributor.author Cox, Helen en_ZA
dc.contributor.author McDermid, Cheryl en_ZA
dc.contributor.author Bosman, Marlein en_ZA
dc.contributor.author van Pittius, Nicolaas C Gey en_ZA
dc.contributor.author Victor, Tommie C en_ZA
dc.contributor.author Kidd, Martin en_ZA
dc.contributor.author van Soolingen, Dick en_ZA
dc.date.accessioned 2015-12-28T06:47:28Z
dc.date.available 2015-12-28T06:47:28Z
dc.date.issued 2013 en_ZA
dc.identifier.citation Hanekom, M., Streicher, E. M., Berg, D. V., Cox, H., McDermid, C., Bosman, M., ... & Warren, R. M. (2013). Population Structure of Mixed Mycobacterium tuberculosis Infection Is Strain Genotype and Culture Medium Dependent. PLoS One, 8(7). doi:10.1371/journal.pone.0070178 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/16028
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0070178
dc.description.abstract BACKGROUND: Molecular genotyping methods have shown infection with more than one Mycobacterium tuberculosis strain genotype in a single sputum culture, indicating mixed infection. Aim This study aimed to develop a PCR-based genotyping tool to determine the population structure of M. tuberculosis strain genotypes in primary Mycobacterial Growth Indicator Tubes (MGIT) and Löwenstein-Jensen (LJ) cultures to identify mixed infections and to establish whether the growth media influenced the recovery of certain strain genotypes. Method A convenience sample of 206 paired MGIT and LJ M. tuberculosis cultures from pulmonary tuberculosis patients resident in Khayelitsha, South Africa were genotyped using an in-house PCR-based method to detect defined M. tuberculosis strain genotypes. RESULTS: The sensitivity and specificity of the PCR-based method for detecting Beijing, Haarlem, S-family, and LAM genotypes was 100%, and 75% and 50% for detecting the Low Copy Clade, respectively. Thirty-one (15%) of the 206 cases showed the presence of more than one M. tuberculosis strain genotype. Strains of the Beijing and Haarlem genotypes were significantly more associated with a mixed infection (on both media) when compared to infections with a single strain (Beijing MGIT p = 0.02; LJ, p<0.01) and (Haarlem: MGIT p<0.01; LJ, p = 0.01). Strains with the Beijing genotype were less likely to be with "other genotype" strains (p<0.01) while LAM, Haarlem, S-family and LCC occurred independently with the Beijing genotype. CONCLUSION: The PCR-based method was able to identify mixed infection in at least 15% of the cases. LJ media was more sensitive in detecting mixed infections than MGIT media, implying that the growth characteristics of M. tuberculosis on different media may influence our ability to detect mixed infections. The Beijing and Haarlem genotypes were more likely to occur in a mixed infection than any of the other genotypes tested suggesting pathogen-pathogen compatibility. en_ZA
dc.language.iso eng en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/4.0 en_ZA
dc.source PLoS One en_ZA
dc.source.uri http://journals.plos.org/plosone en_ZA
dc.subject.other Mycobacterium tuberculosis en_ZA
dc.subject.other Tuberculosis en_ZA
dc.subject.other Polymerase chain reaction en_ZA
dc.subject.other Root structure en_ZA
dc.subject.other Sputum en_ZA
dc.subject.other Genotyping en_ZA
dc.subject.other Culture media en_ZA
dc.subject.other South Africa en_ZA
dc.title Population Structure of Mixed Mycobacterium tuberculosis Infection Is Strain Genotype and Culture Medium Dependent en_ZA
dc.type Journal Article en_ZA
dc.rights.holder © 2013 Hanekom et al en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Institute of Infectious Disease and Molecular Medicine en_ZA
uct.type.filetype Text
uct.type.filetype Image


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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.