Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants

 

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dc.contributor.author Urbino, Cica en_ZA
dc.contributor.author Gutiérrez, Serafin en_ZA
dc.contributor.author Antolik, Anna en_ZA
dc.contributor.author Bouazza, Nabila en_ZA
dc.contributor.author Doumayrou, Juliette en_ZA
dc.contributor.author Granier, Martine en_ZA
dc.contributor.author Martin, Darren P en_ZA
dc.contributor.author Peterschmitt, Michel en_ZA
dc.date.accessioned 2015-12-28T06:47:21Z
dc.date.available 2015-12-28T06:47:21Z
dc.date.issued 2013 en_ZA
dc.identifier.citation Urbino, C., Gutiérrez, S., Antolik, A., Bouazza, N., Doumayrou, J., Granier, M., ... & Peterschmitt, M. (2013). Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants. PLoS ONE, 8(3). doi:10.1371/journal.pone.0058375 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/16023
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0058375
dc.description.abstract Tomato yellow leaf curl virus (TYLCV) is a highly damaging begomovirus native to the Middle East. TYLCV has recently spread worldwide, recombining with other begomoviruses. Recent analysis of mixed infections between TYLCV and Tomato leaf curl Comoros begomovirus (ToLCKMV) has shown that, although natural selection preserves certain co-evolved intra-genomic interactions, numerous and diverse recombinants are produced at 120 days post-inoculation (dpi), and recombinant populations from different tomato plants are very divergent. Here, we investigate the population dynamics that lead to such patterns in tomato plants co-infected with TYLCV and ToLCKMV either by agro-inoculation or using the natural whitefly vector Bemisia tabaci . We monitored the frequency of parental and recombinant genotypes independently in 35 plants between 18 and 330 dpi and identified 177 recombinants isolated at different times. Recombinants were detected from 18 dpi and their frequency increased over time to reach about 50% at 150 dpi regardless of the inoculation method. The distribution of breakpoints detected on 96 fully sequenced recombinants was consistent with a continuous generation of new recombinants as well as random and deterministic effects in their maintenance. A severe population bottleneck of around 10 genomes was estimated during early systemic infection-a phenomenon that could account partially for the heterogeneity in recombinant patterns observed among plants. The detection of the same recombinant genome in six of the thirteen plants analysed beyond 30 dpi supported the influence of selection on observed recombination patterns. Moreover, a highly virulent recombinant genotype dominating virus populations within one plant has, apparently, the potential to be maintained in the natural population according to its infectivity, within-host accumulation, and transmission efficiency - all of which were similar or intermediate to those of the parent genotypes. Our results anticipate the outcomes of natural encounters between TYLCV and ToLCKMV. en_ZA
dc.language.iso eng en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/4.0 en_ZA
dc.source PLoS One en_ZA
dc.source.uri http://journals.plos.org/plosone en_ZA
dc.subject.other Plant genomics en_ZA
dc.subject.other Tomatoes en_ZA
dc.subject.other Leaves en_ZA
dc.subject.other Cloning en_ZA
dc.subject.other Viral genomics en_ZA
dc.subject.other Co-infections en_ZA
dc.subject.other Polymerase chain reaction en_ZA
dc.subject.other Viral vectors en_ZA
dc.title Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants en_ZA
dc.type Journal Article en_ZA
dc.rights.holder © 2013 Urbino et al en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Institute of Infectious Disease and Molecular Medicine en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Urbino, C., Gutiérrez, S., Antolik, A., Bouazza, N., Doumayrou, J., Granier, M., ... Peterschmitt, M. (2013). Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants. <i>PLoS One</i>, http://hdl.handle.net/11427/16023 en_ZA
dc.identifier.chicagocitation Urbino, Cica, Serafin Gutiérrez, Anna Antolik, Nabila Bouazza, Juliette Doumayrou, Martine Granier, Darren P Martin, and Michel Peterschmitt "Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants." <i>PLoS One</i> (2013) http://hdl.handle.net/11427/16023 en_ZA
dc.identifier.vancouvercitation Urbino C, Gutiérrez S, Antolik A, Bouazza N, Doumayrou J, Granier M, et al. Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants. PLoS One. 2013; http://hdl.handle.net/11427/16023. en_ZA
dc.identifier.ris TY - Journal Article AU - Urbino, Cica AU - Gutiérrez, Serafin AU - Antolik, Anna AU - Bouazza, Nabila AU - Doumayrou, Juliette AU - Granier, Martine AU - Martin, Darren P AU - Peterschmitt, Michel AB - Tomato yellow leaf curl virus (TYLCV) is a highly damaging begomovirus native to the Middle East. TYLCV has recently spread worldwide, recombining with other begomoviruses. Recent analysis of mixed infections between TYLCV and Tomato leaf curl Comoros begomovirus (ToLCKMV) has shown that, although natural selection preserves certain co-evolved intra-genomic interactions, numerous and diverse recombinants are produced at 120 days post-inoculation (dpi), and recombinant populations from different tomato plants are very divergent. Here, we investigate the population dynamics that lead to such patterns in tomato plants co-infected with TYLCV and ToLCKMV either by agro-inoculation or using the natural whitefly vector Bemisia tabaci . We monitored the frequency of parental and recombinant genotypes independently in 35 plants between 18 and 330 dpi and identified 177 recombinants isolated at different times. Recombinants were detected from 18 dpi and their frequency increased over time to reach about 50% at 150 dpi regardless of the inoculation method. The distribution of breakpoints detected on 96 fully sequenced recombinants was consistent with a continuous generation of new recombinants as well as random and deterministic effects in their maintenance. A severe population bottleneck of around 10 genomes was estimated during early systemic infection-a phenomenon that could account partially for the heterogeneity in recombinant patterns observed among plants. The detection of the same recombinant genome in six of the thirteen plants analysed beyond 30 dpi supported the influence of selection on observed recombination patterns. Moreover, a highly virulent recombinant genotype dominating virus populations within one plant has, apparently, the potential to be maintained in the natural population according to its infectivity, within-host accumulation, and transmission efficiency - all of which were similar or intermediate to those of the parent genotypes. Our results anticipate the outcomes of natural encounters between TYLCV and ToLCKMV. DA - 2013 DB - OpenUCT DO - 10.1371/journal.pone.0058375 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2013 T1 - Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants TI - Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants UR - http://hdl.handle.net/11427/16023 ER - en_ZA


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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.