Six host-range restricted poxviruses from three genera induce distinct gene expression profiles in an in vivo mouse model

 

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dc.contributor.author Offerman, Kristy en_ZA
dc.contributor.author Deffur, Armin en_ZA
dc.contributor.author Carulei, Olivia en_ZA
dc.contributor.author Wilkinson, Robert en_ZA
dc.contributor.author Douglass, Nicola en_ZA
dc.contributor.author Williamson, Anna Lise en_ZA
dc.date.accessioned 2015-12-07T08:49:41Z
dc.date.available 2015-12-07T08:49:41Z
dc.date.issued 2015 en_ZA
dc.identifier.citation Offerman, K., Deffur, A., Carulei, O., Wilkinson, R., Douglass, N., & Williamson, A. L. (2015). Six host-range restricted poxviruses from three genera induce distinct gene expression profiles in an in vivo mouse model. BMC genomics, 16(1), 510. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/15638
dc.identifier.uri http://dx.doi.org/10.1186/s12864-015-1659-1
dc.description.abstract BACKGROUND: Host-range restricted poxviruses make promising vaccine vectors due to their safety profile and immunogenicity. An understanding of the host innate immune responses produced by different poxvirus vectors would aid in the assessment, selection and rational design of improved vaccines for human and veterinary applications. Novel avipoxviruses are being assessed to determine if they are different from other poxvirus vectors. Analysis of the transcriptome induced in a mouse model would aid in determining if there were significant differences between different poxvirus vectors which may reflect different adjuvant potential as well as establish if they should be further evaluated as vaccine vectors. RESULTS: We compared host transcript abundance in the spleens of BALB/c mice twenty four hours after intravenous infection (10 5 pfu/mouse) with six host-restricted poxvirus species from three genera, namely Lumpy Skin Disease virus (LSDV), Canarypox virus (CNPV), Fowlpox virus (FWPV), modified vaccinia Ankara (MVA) and two novel South African avipoxviruses, Feral Pigeonpox virus (FeP2) and Penguinpox virus (PEPV). These six viruses produced qualitatively and quantitatively distinct host responses with LSDV, followed by MVA, inducing the greatest interferon (IFN) response. FeP2 and PEPV caused very little change to host transcript abundance compared to the other 4 viruses tested. CNPV and FWPV induced the up regulation of two immunoglobulin genes (Ighg and Ighg3 (IgG3)) with CNPV inducing a third, Ighm (IgM). HIV-1-specific IgG3 antibodies have been correlated with decreased risk of HIV-1 infection in the RV144 trial, which included a CNPV-based vector (Yates et al. (Sci Transl Med, 6(228) p228, 2014). Up regulation of IgG3 by CNPV and FWPV but not the other poxviruses tested in vivo, implies that these two avipoxvirus-vector backbones may be involved in stimulation of the clinically important IgG3 antibody subclass. Differential transcript abundance associated with the different poxviruses is further discussed with particular emphasis on responses related to immune responses. CONCLUSION: Six, genetically diverse host-restricted poxviruses produce different responses in a mouse model early after infection. These differences may affect the immune response induced to vaccine antigen in vectors based on these viruses. The two novel avipoxviruses were clearly distinguishable from the other viruses. en_ZA
dc.language.iso eng en_ZA
dc.publisher BioMed Central Ltd en_ZA
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/4.0 en_ZA
dc.source BMC Genomics en_ZA
dc.source.uri http://www.biomedcentral.com/bmcgenomics/ en_ZA
dc.subject.other Poxvirus en_ZA
dc.subject.other Vaccine en_ZA
dc.subject.other Micro-array en_ZA
dc.subject.other LSDV en_ZA
dc.subject.other MVA en_ZA
dc.subject.other Avipoxviruses en_ZA
dc.title Six host-range restricted poxviruses from three genera induce distinct gene expression profiles in an in vivo mouse model en_ZA
dc.type Journal Article en_ZA
dc.rights.holder 2015 Offerman et al. en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Institute of Infectious Disease and Molecular Medicine en_ZA
uct.type.filetype Text
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This is an Open Access article distributed under the terms of the Creative Commons Attribution License Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License