Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus

 

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dc.contributor.advisor Kirby, Ralph en_ZA
dc.contributor.author Botha, Christine Leone en_ZA
dc.date.accessioned 2015-11-30T08:28:32Z
dc.date.available 2015-11-30T08:28:32Z
dc.date.issued 1981 en_ZA
dc.identifier.citation Botha, C. 1981. Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/15447
dc.description Bibliography: leaves 89-96. en_ZA
dc.description.abstract The Actinomycete organism, Streptomyces alboflavus, produces the antibiotic oxytetracycline. Treatment with curing agents resulted in S. alboflavus losing the ability to produce oxytetracycline. However, the loss of oxytetracyc1ine production was reversible, and non-producing colonies regained the ability to produce oxytetracycline when the curing agent was removed. Therefore the loss of oxytetracyc1ine production was not due to the irreversible loss of genetic material specifying oxytetracycline production, but was possibly due to genetic instability. Two extrachromosomal DNA species were isolated from S. alboflavus, (SAP1 and SAP2). SAP1 was approximately 8 - 10 x 10â ¶ da1tons, and appeared to be linear and heterogenous. SAP2 was 20 - 25 x 10â ¶ daltons and appeared to be covalently closed circular. The functions of SAP1 and SAP2 are unknown, although transformation experiments with SAP1 suggested that it may play a role in the production of an antibiotic-like substance, possibly by acting as a promotor of the genes coding for the antibiotic-like substance. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Microbiology en_ZA
dc.title Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus en_ZA
dc.type Master Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Masters
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Botha, C. L. (1981). <i>Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/15447 en_ZA
dc.identifier.chicagocitation Botha, Christine Leone. <i>"Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1981. http://hdl.handle.net/11427/15447 en_ZA
dc.identifier.vancouvercitation Botha CL. Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1981 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/15447 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Botha, Christine Leone AB - The Actinomycete organism, Streptomyces alboflavus, produces the antibiotic oxytetracycline. Treatment with curing agents resulted in S. alboflavus losing the ability to produce oxytetracycline. However, the loss of oxytetracyc1ine production was reversible, and non-producing colonies regained the ability to produce oxytetracycline when the curing agent was removed. Therefore the loss of oxytetracyc1ine production was not due to the irreversible loss of genetic material specifying oxytetracycline production, but was possibly due to genetic instability. Two extrachromosomal DNA species were isolated from S. alboflavus, (SAP1 and SAP2). SAP1 was approximately 8 - 10 x 10â ¶ da1tons, and appeared to be linear and heterogenous. SAP2 was 20 - 25 x 10â ¶ daltons and appeared to be covalently closed circular. The functions of SAP1 and SAP2 are unknown, although transformation experiments with SAP1 suggested that it may play a role in the production of an antibiotic-like substance, possibly by acting as a promotor of the genes coding for the antibiotic-like substance. DA - 1981 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1981 T1 - Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus TI - Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus UR - http://hdl.handle.net/11427/15447 ER - en_ZA


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