Corneal endothelial cells provide evidence of accelerated cellular senescence associated with HIV infection: a case-control study

 

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dc.contributor.author Pathai, Sophia en_ZA
dc.contributor.author Lawn, Stephen D en_ZA
dc.contributor.author Shiels, Paul G en_ZA
dc.contributor.author Weiss, Helen A en_ZA
dc.contributor.author Cook, Colin en_ZA
dc.contributor.author Wood, Robin en_ZA
dc.contributor.author Gilbert, Clare E en_ZA
dc.date.accessioned 2015-11-23T12:25:41Z
dc.date.available 2015-11-23T12:25:41Z
dc.date.issued 2013 en_ZA
dc.identifier.citation Pathai, S., Lawn, S. D., Shiels, P. G., Weiss, H. A., Cook, C., Wood, R., & Gilbert, C. E. (2013). Corneal endothelial cells provide evidence of accelerated cellular senescence associated with HIV infection: a case-control study. PloS one, 8(2), e57422. doi:10.1371/journal.pone.0057422 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/15277
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0057422
dc.description.abstract BACKGROUND: Cellular senescence may be a key factor in HIV-related premature biological aging. We assessed features of the corneal endothelium that are known to be associated with biological aging, and cellular senescence markers in HIV-infected adults. METHODS: Case-control study of 242 HIV-infected adults and 249 matched controls. Using specular microscopy, the corneal endothelium was assessed for features of aging (low endothelial cell density [ECD], high variation in cell size, and low hexagonality index). Data were analysed by multivariable regression. CDKN2A expression (a cell senescence mediator) was measured in peripheral blood leukocytes and 8-hydroxy-2′-deoxyguanosine (8-OHDG; an oxidative DNA damage marker) levels were measured in plasma. RESULTS: The median age of both groups was 40 years. Among HIV-infected adults, 88% were receiving antiretroviral therapy (ART); their median CD4 count was 468 cells/µL. HIV infection was associated with increased odds of variation in cell size (OR = 1.67; 95% CI: 1.00-2.78, p = 0.04). Among HIV-infected participants, low ECD was independently associated with current CD4 count <200 cells/µL (OR = 2.77; 95%CI: 1.12-6.81, p = 0.03). In participants on ART with undetectable viral load, CDKN2A expression and 8-OHDG levels were higher in those with accelerated aging, as reflected by lower ECD. CONCLUSIONS: The corneal endothelium shows features consistent with HIV-related accelerated senescence, especially among those with poor immune recovery. en_ZA
dc.language.iso eng en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/4.0 en_ZA
dc.source PLoS One en_ZA
dc.source.uri http://journals.plos.org/plosone en_ZA
dc.subject.other Endothelial cells en_ZA
dc.subject.other Cornea en_ZA
dc.subject.other Senescence en_ZA
dc.subject.other Eyes en_ZA
dc.subject.other HIV infections en_ZA
dc.subject.other Endothelium en_ZA
dc.subject.other HIV en_ZA
dc.subject.other White blood cells en_ZA
dc.title Corneal endothelial cells provide evidence of accelerated cellular senescence associated with HIV infection: a case-control study en_ZA
dc.type Journal Article en_ZA
dc.rights.holder © 2013 Pathai et al en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Desmond Tutu HIV Centre en_ZA
uct.type.filetype Text
uct.type.filetype Image


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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.