Unsaturated glycerophospholipids mediate heme crystallization: biological implications for hemozoin formation in the kissing bug Rhodnius prolixus

 

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dc.contributor.author Stiebler, Renata en_ZA
dc.contributor.author Majerowicz, David en_ZA
dc.contributor.author Knudsen, Jens en_ZA
dc.contributor.author Gondim, Katia C en_ZA
dc.contributor.author Wright, David W en_ZA
dc.contributor.author Egan, Timothy J en_ZA
dc.contributor.author Oliveira, Marcus F en_ZA
dc.date.accessioned 2015-11-18T07:13:16Z
dc.date.available 2015-11-18T07:13:16Z
dc.date.issued 2014 en_ZA
dc.identifier.citation Stiebler, R., Majerowicz, D., Knudsen, J., Gondim, K. C., Wright, D. W., Egan, T. J., & Oliveira, M. F. (2013). Unsaturated glycerophospholipids mediate heme crystallization: biological implications for hemozoin formation in the kissing bug Rhodnius prolixus. PloS one, 9(2), e88976. doi:10.1371/journal.pone.0088976 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/15154
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0088976
dc.description.abstract Hemozoin (Hz) is a heme crystal produced by some blood-feeding organisms, as an efficient way to detoxify heme derived from hemoglobin digestion. In the triatomine insect Rhodnius prolixus , Hz is essentially produced by midgut extracellular phospholipid membranes known as perimicrovillar membranes (PMVM). Here, we investigated the role of commercial glycerophospholipids containing serine, choline and ethanolamine as headgroups and R. prolixus midgut lipids (RML) in heme crystallization. All commercial unsaturated forms of phospholipids, as well as RML, mediated fast and efficient β-hematin formation by means of two kinetically distinct mechanisms: an early and fast component, followed by a late and slow one. The fastest reactions observed were induced by unsaturated forms of phosphatidylethanolamine (uPE) and phosphatidylcholine (uPC), with half-lives of 0.04 and 0.7 minutes, respectively. β-hematin crystal morphologies were strikingly distinct among groups, with uPE producing homogeneous regular brick-shaped crystals. Interestingly, uPC-mediated reactions resulted in two morphologically distinct crystal populations: one less representative group of regular crystals, resembling those induced by uPE, and the other largely represented by crystals with numerous sharp edges and tapered ends. Heme crystallization reactions induced by RML were efficient, with a heme to β-hematin conversion rate higher than 70%, but clearly slower (t1/2 of 9.9-17.7 minutes) than those induced by uPC and uPE. Interestingly, crystals produced by RML were homogeneous in shape and quite similar to those mediated by uPE. Thus, β-hematin formation can be rapidly and efficiently induced by unsaturated glycerophospholipids, particularly uPE and uPC, and may play a role on biological heme crystallization in R. prolixus midgut. en_ZA
dc.language.iso eng en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/4.0 en_ZA
dc.source PLoS One en_ZA
dc.source.uri http://journals.plos.org/plosone en_ZA
dc.subject.other Heme en_ZA
dc.subject.other Phospholipids en_ZA
dc.subject.other Lipids en_ZA
dc.subject.other Crystals en_ZA
dc.subject.other Crystallization en_ZA
dc.subject.other Insects en_ZA
dc.subject.other Blood plasma en_ZA
dc.subject.other Crystal structure en_ZA
dc.title Unsaturated glycerophospholipids mediate heme crystallization: biological implications for hemozoin formation in the kissing bug Rhodnius prolixus en_ZA
dc.type Journal Article en_ZA
dc.rights.holder © 2014 Stiebler et al en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Chemistry en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Stiebler, R., Majerowicz, D., Knudsen, J., Gondim, K. C., Wright, D. W., Egan, T. J., & Oliveira, M. F. (2014). Unsaturated glycerophospholipids mediate heme crystallization: biological implications for hemozoin formation in the kissing bug Rhodnius prolixus. <i>PLoS One</i>, http://hdl.handle.net/11427/15154 en_ZA
dc.identifier.chicagocitation Stiebler, Renata, David Majerowicz, Jens Knudsen, Katia C Gondim, David W Wright, Timothy J Egan, and Marcus F Oliveira "Unsaturated glycerophospholipids mediate heme crystallization: biological implications for hemozoin formation in the kissing bug Rhodnius prolixus." <i>PLoS One</i> (2014) http://hdl.handle.net/11427/15154 en_ZA
dc.identifier.vancouvercitation Stiebler R, Majerowicz D, Knudsen J, Gondim KC, Wright DW, Egan TJ, et al. Unsaturated glycerophospholipids mediate heme crystallization: biological implications for hemozoin formation in the kissing bug Rhodnius prolixus. PLoS One. 2014; http://hdl.handle.net/11427/15154. en_ZA
dc.identifier.ris TY - Journal Article AU - Stiebler, Renata AU - Majerowicz, David AU - Knudsen, Jens AU - Gondim, Katia C AU - Wright, David W AU - Egan, Timothy J AU - Oliveira, Marcus F AB - Hemozoin (Hz) is a heme crystal produced by some blood-feeding organisms, as an efficient way to detoxify heme derived from hemoglobin digestion. In the triatomine insect Rhodnius prolixus , Hz is essentially produced by midgut extracellular phospholipid membranes known as perimicrovillar membranes (PMVM). Here, we investigated the role of commercial glycerophospholipids containing serine, choline and ethanolamine as headgroups and R. prolixus midgut lipids (RML) in heme crystallization. All commercial unsaturated forms of phospholipids, as well as RML, mediated fast and efficient β-hematin formation by means of two kinetically distinct mechanisms: an early and fast component, followed by a late and slow one. The fastest reactions observed were induced by unsaturated forms of phosphatidylethanolamine (uPE) and phosphatidylcholine (uPC), with half-lives of 0.04 and 0.7 minutes, respectively. β-hematin crystal morphologies were strikingly distinct among groups, with uPE producing homogeneous regular brick-shaped crystals. Interestingly, uPC-mediated reactions resulted in two morphologically distinct crystal populations: one less representative group of regular crystals, resembling those induced by uPE, and the other largely represented by crystals with numerous sharp edges and tapered ends. Heme crystallization reactions induced by RML were efficient, with a heme to β-hematin conversion rate higher than 70%, but clearly slower (t1/2 of 9.9-17.7 minutes) than those induced by uPC and uPE. Interestingly, crystals produced by RML were homogeneous in shape and quite similar to those mediated by uPE. Thus, β-hematin formation can be rapidly and efficiently induced by unsaturated glycerophospholipids, particularly uPE and uPC, and may play a role on biological heme crystallization in R. prolixus midgut. DA - 2014 DB - OpenUCT DO - 10.1371/journal.pone.0088976 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - Unsaturated glycerophospholipids mediate heme crystallization: biological implications for hemozoin formation in the kissing bug Rhodnius prolixus TI - Unsaturated glycerophospholipids mediate heme crystallization: biological implications for hemozoin formation in the kissing bug Rhodnius prolixus UR - http://hdl.handle.net/11427/15154 ER - en_ZA


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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.