East African cassava mosaic-like viruses from Africa to Indian ocean islands: molecular diversity, evolutionary history and geographical dissemination of a bipartite begomovirus

 

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dc.contributor.author De Bruyn, Alexandre en_ZA
dc.contributor.author Villemot, Julie en_ZA
dc.contributor.author Lefeuvre, Pierre en_ZA
dc.contributor.author Villar, Emilie en_ZA
dc.contributor.author Hoareau, Murielle en_ZA
dc.contributor.author Harimalala, Mireille en_ZA
dc.contributor.author Abdoul-Karime, Anli en_ZA
dc.contributor.author Abdou-Chakour, Chadhouliati en_ZA
dc.contributor.author Reynaud, Bernard en_ZA
dc.contributor.author Harkins, Gordon en_ZA
dc.contributor.author Varsani, Arvind en_ZA
dc.contributor.author Martin, Darren en_ZA
dc.contributor.author Lett, Jean-Michel en_ZA
dc.date.accessioned 2015-11-18T03:58:39Z
dc.date.available 2015-11-18T03:58:39Z
dc.date.issued 2012 en_ZA
dc.identifier.citation De Bruyn, A., Villemot, J., Lefeuvre, P., Villar, E., Hoareau, M., Harimalala, M., ... & Lett, J. M. (2012). East African cassava mosaic-like viruses from Africa to Indian ocean islands: molecular diversity, evolutionary history and geographical dissemination of a bipartite begomovirus. BMC evolutionary biology, 12(1), 228. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/15075
dc.identifier.uri http://dx.doi.org/10.1186/1471-2148-12-228
dc.description.abstract BACKGROUND: Cassava (Manihot esculenta) is a major food source for over 200 million sub-Saharan Africans. Unfortunately, its cultivation is severely hampered by cassava mosaic disease (CMD). Caused by a complex of bipartite cassava mosaic geminiviruses (CMG) species (Family: Geminivirideae; Genus: Begomovirus) CMD has been widely described throughout Africa and it is apparent that CMG's are expanding their geographical distribution. Determining where and when CMG movements have occurred could help curtail its spread and reveal the ecological and anthropic factors associated with similar viral invasions. We applied Bayesian phylogeographic inference and recombination analyses to available and newly described CMG sequences to reconstruct a plausible history of CMG diversification and migration between Africa and South West Indian Ocean (SWIO) islands. RESULTS: The isolation and analysis of 114 DNA-A and 41 DNA-B sequences demonstrated the presence of three CMG species circulating in the Comoros and Seychelles archipelagos (East African cassava mosaic virus, EACMV; East African cassava mosaic Kenya virus, EACMKV; and East African cassava mosaic Cameroon virus, EACMCV). Phylogeographic analyses suggest that CMG's presence on these SWIO islands is probably the result of at least four independent introduction events from mainland Africa occurring between 1988 and 2009. Amongst the islands of the Comoros archipelago, two major migration pathways were inferred: One from Grande Comore to Moheli and the second from Mayotte to Anjouan. While only two recombination events characteristic of SWIO islands isolates were identified, numerous re-assortments events were detected between EACMV and EACMKV, which seem to almost freely interchange their genome components. CONCLUSIONS: Rapid and extensive virus spread within the SWIO islands was demonstrated for three CMG complex species. Strong evolutionary or ecological interaction between CMG species may explain both their propensity to exchange components and the absence of recombination with non-CMG begomoviruses. Our results suggest an important role of anthropic factors in CMGs spread as the principal axes of viral migration correspond with major routes of human movement and commercial trade. Finer-scale temporal analyses of CMGs to precisely scale the relative contributions of human and insect transmission to their movement dynamics will require further extensive sampling in the SWIO region. en_ZA
dc.language.iso eng en_ZA
dc.publisher BioMed Central Ltd en_ZA
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_ZA
dc.source BMC Evolutionary Biology en_ZA
dc.source.uri http://www.biomedcentral.com/bmcevolbiol/ en_ZA
dc.subject.other Cassava (Manihot esculenta) en_ZA
dc.subject.other sub-Saharan Africa en_ZA
dc.subject.other cassava mosaic disease (CMD) en_ZA
dc.title East African cassava mosaic-like viruses from Africa to Indian ocean islands: molecular diversity, evolutionary history and geographical dissemination of a bipartite begomovirus en_ZA
dc.type Journal Article en_ZA
dc.rights.holder 2012 De Bruyn et al.; licensee BioMed Central Ltd. en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Institute of Infectious Disease and Molecular Medicine en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation De Bruyn, A., Villemot, J., Lefeuvre, P., Villar, E., Hoareau, M., Harimalala, M., ... Lett, J. (2012). East African cassava mosaic-like viruses from Africa to Indian ocean islands: molecular diversity, evolutionary history and geographical dissemination of a bipartite begomovirus. <i>BMC Evolutionary Biology</i>, http://hdl.handle.net/11427/15075 en_ZA
dc.identifier.chicagocitation De Bruyn, Alexandre, Julie Villemot, Pierre Lefeuvre, Emilie Villar, Murielle Hoareau, Mireille Harimalala, Anli Abdoul-Karime, et al "East African cassava mosaic-like viruses from Africa to Indian ocean islands: molecular diversity, evolutionary history and geographical dissemination of a bipartite begomovirus." <i>BMC Evolutionary Biology</i> (2012) http://hdl.handle.net/11427/15075 en_ZA
dc.identifier.vancouvercitation De Bruyn A, Villemot J, Lefeuvre P, Villar E, Hoareau M, Harimalala M, et al. East African cassava mosaic-like viruses from Africa to Indian ocean islands: molecular diversity, evolutionary history and geographical dissemination of a bipartite begomovirus. BMC Evolutionary Biology. 2012; http://hdl.handle.net/11427/15075. en_ZA
dc.identifier.ris TY - Journal Article AU - De Bruyn, Alexandre AU - Villemot, Julie AU - Lefeuvre, Pierre AU - Villar, Emilie AU - Hoareau, Murielle AU - Harimalala, Mireille AU - Abdoul-Karime, Anli AU - Abdou-Chakour, Chadhouliati AU - Reynaud, Bernard AU - Harkins, Gordon AU - Varsani, Arvind AU - Martin, Darren AU - Lett, Jean-Michel AB - BACKGROUND: Cassava (Manihot esculenta) is a major food source for over 200 million sub-Saharan Africans. Unfortunately, its cultivation is severely hampered by cassava mosaic disease (CMD). Caused by a complex of bipartite cassava mosaic geminiviruses (CMG) species (Family: Geminivirideae; Genus: Begomovirus) CMD has been widely described throughout Africa and it is apparent that CMG's are expanding their geographical distribution. Determining where and when CMG movements have occurred could help curtail its spread and reveal the ecological and anthropic factors associated with similar viral invasions. We applied Bayesian phylogeographic inference and recombination analyses to available and newly described CMG sequences to reconstruct a plausible history of CMG diversification and migration between Africa and South West Indian Ocean (SWIO) islands. RESULTS: The isolation and analysis of 114 DNA-A and 41 DNA-B sequences demonstrated the presence of three CMG species circulating in the Comoros and Seychelles archipelagos (East African cassava mosaic virus, EACMV; East African cassava mosaic Kenya virus, EACMKV; and East African cassava mosaic Cameroon virus, EACMCV). Phylogeographic analyses suggest that CMG's presence on these SWIO islands is probably the result of at least four independent introduction events from mainland Africa occurring between 1988 and 2009. Amongst the islands of the Comoros archipelago, two major migration pathways were inferred: One from Grande Comore to Moheli and the second from Mayotte to Anjouan. While only two recombination events characteristic of SWIO islands isolates were identified, numerous re-assortments events were detected between EACMV and EACMKV, which seem to almost freely interchange their genome components. CONCLUSIONS: Rapid and extensive virus spread within the SWIO islands was demonstrated for three CMG complex species. Strong evolutionary or ecological interaction between CMG species may explain both their propensity to exchange components and the absence of recombination with non-CMG begomoviruses. Our results suggest an important role of anthropic factors in CMGs spread as the principal axes of viral migration correspond with major routes of human movement and commercial trade. Finer-scale temporal analyses of CMGs to precisely scale the relative contributions of human and insect transmission to their movement dynamics will require further extensive sampling in the SWIO region. DA - 2012 DB - OpenUCT DO - 10.1186/1471-2148-12-228 DP - University of Cape Town J1 - BMC Evolutionary Biology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - East African cassava mosaic-like viruses from Africa to Indian ocean islands: molecular diversity, evolutionary history and geographical dissemination of a bipartite begomovirus TI - East African cassava mosaic-like viruses from Africa to Indian ocean islands: molecular diversity, evolutionary history and geographical dissemination of a bipartite begomovirus UR - http://hdl.handle.net/11427/15075 ER - en_ZA


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