Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus

 

Show simple item record

dc.contributor.author Monjane, Aderito en_ZA
dc.contributor.author Pande, Daniel en_ZA
dc.contributor.author Lakay, Francisco en_ZA
dc.contributor.author Shepherd, Dionne en_ZA
dc.contributor.author van der Walt, Eric en_ZA
dc.contributor.author Lefeuvre, Pierre en_ZA
dc.contributor.author Lett, Jean-Michel en_ZA
dc.contributor.author Varsani, Arvind en_ZA
dc.contributor.author Rybicki, Edward en_ZA
dc.contributor.author Martin, Darren en_ZA
dc.date.accessioned 2015-11-18T03:58:33Z
dc.date.available 2015-11-18T03:58:33Z
dc.date.issued 2012 en_ZA
dc.identifier.citation Monjane, A. L., Pande, D., Lakay, F., Shepherd, D. N., van der Walt, E., Lefeuvre, P., ... & Martin, D. P. (2012). Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus. BMC evolutionary biology, 12(1), 252. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/15074
dc.identifier.uri http://dx.doi.org/10.1186/1471-2148-12-252
dc.description.abstract BACKGROUND: Single-stranded (ss) DNA viruses in the family Geminiviridae are proving to be very useful in real-time evolution studies. The high mutation rate of geminiviruses and other ssDNA viruses is somewhat mysterious in that their DNA genomes are replicated in host nuclei by high fidelity host polymerases. Although strand specific mutation biases observed in virus species from the geminivirus genus Mastrevirus indicate that the high mutation rates in viruses in this genus may be due to mutational processes that operate specifically on ssDNA, it is currently unknown whether viruses from other genera display similar strand specific mutation biases. Also, geminivirus genomes frequently recombine with one another and an alternative cause of their high mutation rates could be that the recombination process is either directly mutagenic or produces a selective environment in which the survival of mutants is favoured. To investigate whether there is an association between recombination and increased basal mutation rates or increased degrees of selection favoring the survival of mutations, we compared the mutation dynamics of the MSV-MatA and MSV-VW field isolates of Maize streak virus (MSV; Mastrevirus), with both a laboratory constructed MSV recombinant, and MSV recombinants closely resembling MSV-MatA. To determine whether strand specific mutation biases are a general characteristic of geminivirus evolution we compared mutation spectra arising during these MSV experiments with those arising during similar experiments involving the geminivirus Tomato yellow leaf curl virus (Begomovirus genus). RESULTS: Although both the genomic distribution of mutations and the occurrence of various convergent mutations at specific genomic sites indicated that either mutation hotspots or selection for adaptive mutations might elevate observed mutation rates in MSV, we found no association between recombination and mutation rates. Importantly, when comparing the mutation spectra of MSV and TYLCV we observed similar strand specific mutation biases arising predominantly from imbalances in the complementary mutations G->T: C->A. CONCLUSIONS: While our results suggest that recombination does not strongly influence mutation rates in MSV, they indicate that high geminivirus mutation rates are at least partially attributable to increased susceptibility of all geminivirus genomes to oxidative damage while in a single stranded state. en_ZA
dc.language.iso eng en_ZA
dc.publisher BioMed Central Ltd en_ZA
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_ZA
dc.source BMC Evolutionary Biology en_ZA
dc.source.uri http://www.biomedcentral.com/bmcevolbiol/ en_ZA
dc.subject.other Single-stranded (ss) DNA viruses en_ZA
dc.subject.other geminivirus genus Mastrevirus en_ZA
dc.title Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus en_ZA
dc.type Journal Article en_ZA
dc.rights.holder 2012 Monjane et al.; licensee BioMed Central Ltd. en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Institute of Infectious Disease and Molecular Medicine en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Monjane, A., Pande, D., Lakay, F., Shepherd, D., van der Walt, E., Lefeuvre, P., ... Martin, D. (2012). Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus. <i>BMC Evolutionary Biology</i>, http://hdl.handle.net/11427/15074 en_ZA
dc.identifier.chicagocitation Monjane, Aderito, Daniel Pande, Francisco Lakay, Dionne Shepherd, Eric van der Walt, Pierre Lefeuvre, Jean-Michel Lett, Arvind Varsani, Edward Rybicki, and Darren Martin "Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus." <i>BMC Evolutionary Biology</i> (2012) http://hdl.handle.net/11427/15074 en_ZA
dc.identifier.vancouvercitation Monjane A, Pande D, Lakay F, Shepherd D, van der Walt E, Lefeuvre P, et al. Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus. BMC Evolutionary Biology. 2012; http://hdl.handle.net/11427/15074. en_ZA
dc.identifier.ris TY - Journal Article AU - Monjane, Aderito AU - Pande, Daniel AU - Lakay, Francisco AU - Shepherd, Dionne AU - van der Walt, Eric AU - Lefeuvre, Pierre AU - Lett, Jean-Michel AU - Varsani, Arvind AU - Rybicki, Edward AU - Martin, Darren AB - BACKGROUND: Single-stranded (ss) DNA viruses in the family Geminiviridae are proving to be very useful in real-time evolution studies. The high mutation rate of geminiviruses and other ssDNA viruses is somewhat mysterious in that their DNA genomes are replicated in host nuclei by high fidelity host polymerases. Although strand specific mutation biases observed in virus species from the geminivirus genus Mastrevirus indicate that the high mutation rates in viruses in this genus may be due to mutational processes that operate specifically on ssDNA, it is currently unknown whether viruses from other genera display similar strand specific mutation biases. Also, geminivirus genomes frequently recombine with one another and an alternative cause of their high mutation rates could be that the recombination process is either directly mutagenic or produces a selective environment in which the survival of mutants is favoured. To investigate whether there is an association between recombination and increased basal mutation rates or increased degrees of selection favoring the survival of mutations, we compared the mutation dynamics of the MSV-MatA and MSV-VW field isolates of Maize streak virus (MSV; Mastrevirus), with both a laboratory constructed MSV recombinant, and MSV recombinants closely resembling MSV-MatA. To determine whether strand specific mutation biases are a general characteristic of geminivirus evolution we compared mutation spectra arising during these MSV experiments with those arising during similar experiments involving the geminivirus Tomato yellow leaf curl virus (Begomovirus genus). RESULTS: Although both the genomic distribution of mutations and the occurrence of various convergent mutations at specific genomic sites indicated that either mutation hotspots or selection for adaptive mutations might elevate observed mutation rates in MSV, we found no association between recombination and mutation rates. Importantly, when comparing the mutation spectra of MSV and TYLCV we observed similar strand specific mutation biases arising predominantly from imbalances in the complementary mutations G->T: C->A. CONCLUSIONS: While our results suggest that recombination does not strongly influence mutation rates in MSV, they indicate that high geminivirus mutation rates are at least partially attributable to increased susceptibility of all geminivirus genomes to oxidative damage while in a single stranded state. DA - 2012 DB - OpenUCT DO - 10.1186/1471-2148-12-252 DP - University of Cape Town J1 - BMC Evolutionary Biology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus TI - Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus UR - http://hdl.handle.net/11427/15074 ER - en_ZA


Files in this item

This item appears in the following Collection(s)

Show simple item record

This is an Open Access article distributed under the terms of the Creative Commons Attribution License Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License