Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock

 

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dc.contributor.author Bhardwaj, Vaibhav en_ZA
dc.contributor.author Meier, Stuart en_ZA
dc.contributor.author Petersen, Lindsay N en_ZA
dc.contributor.author Ingle, Robert A en_ZA
dc.contributor.author Roden, Laura C en_ZA
dc.date.accessioned 2015-11-16T04:12:29Z
dc.date.available 2015-11-16T04:12:29Z
dc.date.issued 2011 en_ZA
dc.identifier.citation Bhardwaj, V., Meier, S., Petersen, L. N., Ingle, R. A., & Roden, L. C. (2011). Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock. PloS one, 6(10), e26968-e26968. doi:10.1371/journal.pone.0026968 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/15025
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0026968
dc.description.abstract The circadian clock allows plants to anticipate predictable daily changes in abiotic stimuli, such as light; however, whether the clock similarly allows plants to anticipate interactions with other organisms is unknown. Here we show that Arabidopsis thaliana (Arabidopsis) has circadian clock-mediated variation in resistance to the virulent bacterial pathogen Pseudomonas syringae pv. tomato DC3000 ( Pst DC3000), with plants being least susceptible to infection in the subjective morning. We suggest that the increased resistance to Pst DC3000 observed in the morning in Col-0 plants results from clock-mediated modulation of pathogen associated molecular pattern (PAMP)-triggered immunity. Analysis of publicly available microarray data revealed that a large number of Arabidopsis defence-related genes showed both diurnal- and circadian-regulation, including genes involved in the perception of the PAMP flagellin which exhibit a peak in expression in the morning. Accordingly, we observed that PAMP-triggered callose deposition was significantly higher in wild-type plants inoculated with Pst DC3000 hrpA in the subjective morning than in the evening, while no such temporal difference was evident in arrhythmic plants. Our results suggest that PAMP-triggered immune responses are modulated by the circadian clock and that temporal regulation allows plants to anticipate and respond more effectively to pathogen challenges in the daytime. en_ZA
dc.language.iso eng en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/4.0 en_ZA
dc.source PLoS One en_ZA
dc.source.uri http://journals.plos.org/plosone en_ZA
dc.subject.other Circadian rhythms en_ZA
dc.subject.other Circadian oscillators en_ZA
dc.subject.other Gene regulation en_ZA
dc.subject.other Gene expression en_ZA
dc.subject.other Plant pathogens en_ZA
dc.subject.other Plant bacterial pathogens en_ZA
dc.subject.other Pseudomonas infections en_ZA
dc.subject.other Arabidopsis thaliana en_ZA
dc.title Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock en_ZA
dc.type Journal Article en_ZA
dc.rights.holder © 2011 Bhardwaj et al en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
uct.type.filetype Text
uct.type.filetype Image


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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.