Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts

 

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dc.contributor.author Habte, Habtom en_ZA
dc.contributor.author de Beer, Corena en_ZA
dc.contributor.author Lotz, Zoe en_ZA
dc.contributor.author Roux, Paul en_ZA
dc.contributor.author Mall, Anwar en_ZA
dc.date.accessioned 2015-11-11T11:56:13Z
dc.date.available 2015-11-11T11:56:13Z
dc.date.issued 2010 en_ZA
dc.identifier.citation Habte, H. H., De Beer, C., Lotz, Z. E., Roux, P., & Mall, A. S. (2010). Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts. Virol J, 7, 269. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/14865
dc.identifier.uri http://dx.doi.org/10.1186/1743-422X-7-269
dc.description.abstract BACKGROUND: We have previously shown that MUC5B and MUC7 mucins from saliva of HIV negative individuals inhibit HIV-1 activity by 100% in an in vitro assay. The purpose of this subsequent study was to investigate whether MUC5B and MUC7 from saliva of HIV patients or with full blown AIDS had a similar inhibitory activity against the virus. METHODS: Salivary MUC5B and MUC7 from HIV patients with different CD4 counts (< 200, 200-400 and > 400) were incubated with HIV-1 prior to infection of the human T lymphoblastoid cell line (CEM SS cells). Cells were then cultured and viral replication was measured by a qualitative p24 antigen assay. The size, charge and immunoreactivity of mucins from HIV negative and positive individuals was also analysed by SDS-PAGE, Western blot and ELISA respectively. RESULTS: It was shown that irrespective of their CD4 counts both MUC5B and MUC7 from HIV patients, unlike the MUC5B and MUC7 from HIV negative individuals, did not inhibit HIV-1 activity. Size, charge and immunoreactivity differences between the mucins from HIV negative and positive individuals and among the mucins from HIV patients of different CD4 count was observed by SDS-PAGE, Western blot and ELISA. CONCLUSIONS: Purified salivary mucins from HIV positive patients do not inhibit the AIDS virus in an in vitro assay. Although the reason for the inability of mucins from infected individuals to inhibit the virus is not known, it is likely that there is an alteration of the glycosylation pattern, and therefore of charge of mucin, in HIV positive patients. The ability to inhibit the virus by aggregation by sugar chains is thus diminished. en_ZA
dc.language.iso eng en_ZA
dc.publisher BioMed Central Ltd en_ZA
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_ZA
dc.source Virology Journal en_ZA
dc.source.uri http://www.virologyj.com/ en_ZA
dc.subject.other Human saliva, HIV transmission, HIV (Viruses), Oral transmission, HIV (Viruses) Research en_ZA
dc.title Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts en_ZA
dc.type Journal Article en_ZA
dc.rights.holder 2010 Habte et al; licensee BioMed Central Ltd. en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Division of General Surgery en_ZA
uct.type.filetype Text
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This is an Open Access article distributed under the terms of the Creative Commons Attribution License Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License