Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses

 

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dc.contributor.author Checkley, Anna M. en_ZA
dc.contributor.author Wyllie, David H. en_ZA
dc.contributor.author Scriba, Thomas J. en_ZA
dc.contributor.author Golubchik, Tanya en_ZA
dc.contributor.author Hill, Adrian V. S. en_ZA
dc.contributor.author Hanekom, Willem A. en_ZA
dc.contributor.author McShane, Helen en_ZA
dc.date.accessioned 2015-11-09T13:22:18Z
dc.date.available 2015-11-09T13:22:18Z
dc.date.issued 2011 en_ZA
dc.identifier.citation Checkley, A. M., Wyllie, D. H., Scriba, T. J., Golubchik, T., Hill, A. V., Hanekom, W. A., & McShane, H. (2011). Identification of antigens specific to nontuberculous mycobacteria: the Mce family of proteins as a target of T cell immune responses. PloS one, 6(10), e26434. doi:10.1371/journal.pone.0026434 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/14790
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0026434
dc.description.abstract The lack of an effective TB vaccine hinders current efforts in combating the TB pandemic. One theory as to why BCG is less protective in tropical countries is that exposure to non-tuberculous mycobacteria (NTM) reduces BCG efficacy. There are currently several new TB vaccines in clinical trials, and NTM exposure may also be relevant in this context. NTM exposure cannot be accurately evaluated in the absence of specific antigens; those which are known to be present in NTM and absent from M. tuberculosis and BCG. We therefore used a bioinformatic pipeline to define proteins which are present in common NTM and absent from the M. tuberculosis complex, using protein BLAST, TBLASTN and a short sequence protein BLAST to ensure the specificity of this process. We then assessed immune responses to these proteins, in healthy South Africans and in patients from the United Kingdom and United States with documented exposure to NTM. Low level responses were detected to a cluster of proteins from the mammalian cell entry family, and to a cluster of hypothetical proteins, using ex vivo ELISpot and a 6 day proliferation assay. These early findings may provide a basis for characterising exposure to NTM at a population level, which has applications in the field of TB vaccine design as well as in the development of diagnostic tests. en_ZA
dc.language.iso eng en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/4.0 en_ZA
dc.source PLoS One en_ZA
dc.source.uri http://journals.plos.org/plosone en_ZA
dc.subject.other Mycobacterium tuberculosis en_ZA
dc.subject.other Mycobacterium avium complex en_ZA
dc.subject.other Mycobacterium ulcerans en_ZA
dc.subject.other Enzyme-linked immunoassays en_ZA
dc.subject.other Tuberculosis en_ZA
dc.subject.other BLAST algorithm en_ZA
dc.subject.other Immune response en_ZA
dc.subject.other T cells en_ZA
dc.title Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses en_ZA
dc.type Journal Article en_ZA
dc.rights.holder © 2011 Checkley et al en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Department of Paediatrics and Child Health en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Checkley, Anna M., Wyllie, David H., Scriba, Thomas J., Golubchik, T., Hill, Adrian V. S., Hanekom, Willem A., & McShane, H. (2011). Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses. <i>PLoS One</i>, http://hdl.handle.net/11427/14790 en_ZA
dc.identifier.chicagocitation Checkley, Anna M., David H. Wyllie, Thomas J. Scriba, Tanya Golubchik, Adrian V. S. Hill, Willem A. Hanekom, and Helen McShane "Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses." <i>PLoS One</i> (2011) http://hdl.handle.net/11427/14790 en_ZA
dc.identifier.vancouvercitation Checkley Anna M, Wyllie David H, Scriba Thomas J, Golubchik T, Hill Adrian V S, Hanekom Willem A, et al. Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses. PLoS One. 2011; http://hdl.handle.net/11427/14790. en_ZA
dc.identifier.ris TY - Journal Article AU - Checkley, Anna M. AU - Wyllie, David H. AU - Scriba, Thomas J. AU - Golubchik, Tanya AU - Hill, Adrian V. S. AU - Hanekom, Willem A. AU - McShane, Helen AB - The lack of an effective TB vaccine hinders current efforts in combating the TB pandemic. One theory as to why BCG is less protective in tropical countries is that exposure to non-tuberculous mycobacteria (NTM) reduces BCG efficacy. There are currently several new TB vaccines in clinical trials, and NTM exposure may also be relevant in this context. NTM exposure cannot be accurately evaluated in the absence of specific antigens; those which are known to be present in NTM and absent from M. tuberculosis and BCG. We therefore used a bioinformatic pipeline to define proteins which are present in common NTM and absent from the M. tuberculosis complex, using protein BLAST, TBLASTN and a short sequence protein BLAST to ensure the specificity of this process. We then assessed immune responses to these proteins, in healthy South Africans and in patients from the United Kingdom and United States with documented exposure to NTM. Low level responses were detected to a cluster of proteins from the mammalian cell entry family, and to a cluster of hypothetical proteins, using ex vivo ELISpot and a 6 day proliferation assay. These early findings may provide a basis for characterising exposure to NTM at a population level, which has applications in the field of TB vaccine design as well as in the development of diagnostic tests. DA - 2011 DB - OpenUCT DO - 10.1371/journal.pone.0026434 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses TI - Identification of Antigens Specific to Non-Tuberculous Mycobacteria: The Mce Family of Proteins as a Target of T Cell Immune Responses UR - http://hdl.handle.net/11427/14790 ER - en_ZA


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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.