Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes

 

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dc.contributor.author Bryan, Christopher en_ZA
dc.contributor.author Marchal, Marie en_ZA
dc.contributor.author Battaglia-Brunet, Fabienne en_ZA
dc.contributor.author Kugler, Valerie en_ZA
dc.contributor.author Lemaitre-Guillier, Christelle en_ZA
dc.contributor.author Lievremont, Didier en_ZA
dc.contributor.author Bertin, Philippe en_ZA
dc.contributor.author Arsene-Ploetze, Florence en_ZA
dc.date.accessioned 2015-10-30T09:38:29Z
dc.date.available 2015-10-30T09:38:29Z
dc.date.issued 2009 en_ZA
dc.identifier.citation Bryan, C. G., Marchal, M., Battaglia-Brunet, F., Kugler, V., Lemaitre-Guillier, C., Lièvremont, D., ... & Arsène-Ploetze, F. (2009). Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes. BMC microbiology, 9(1), 127. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/14551
dc.identifier.uri http://dx.doi.org/10.1186/1471-2180-9-127
dc.description.abstract BACKGROUND:Thiomonas strains are ubiquitous in arsenic-contaminated environments. Differences between Thiomonas strains in the way they have adapted and respond to arsenic have never been studied in detail. For this purpose, five Thiomonas strains, that are interesting in terms of arsenic metabolism were selected: T. arsenivorans, Thiomonas spp. WJ68 and 3As are able to oxidise As(III), while Thiomonas sp. Ynys1 and T. perometabolis are not. Moreover, T. arsenivorans and 3As present interesting physiological traits, in particular that these strains are able to use As(III) as an electron donor. RESULTS: The metabolism of carbon and arsenic was compared in the five Thiomonas strains belonging to two distinct phylogenetic groups. Greater physiological differences were found between these strains than might have been suggested by 16S rRNA/rpoA gene phylogeny, especially regarding arsenic metabolism. Physiologically, T. perometabolis and Ynys1 were unable to oxidise As(III) and were less arsenic-resistant than the other strains. Genetically, they appeared to lack the aox arsenic-oxidising genes and carried only a single ars arsenic resistance operon. Thiomonas arsenivorans belonged to a distinct phylogenetic group and increased its autotrophic metabolism when arsenic concentration increased. Differential proteomic analysis revealed that in T. arsenivorans, the rbc/cbb genes involved in the assimilation of inorganic carbon were induced in the presence of arsenic, whereas these genes were repressed in Thiomonas sp. 3As. CONCLUSION: Taken together, these results show that these closely related bacteria differ substantially in their response to arsenic, amongst other factors, and suggest different relationships between carbon assimilation and arsenic metabolism. en_ZA
dc.language.iso eng en_ZA
dc.publisher BioMed Central Ltd en_ZA
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_ZA
dc.source BMC Microbiology en_ZA
dc.source.uri http://www.biomedcentral.com/bmcmicrobiol/ en_ZA
dc.subject.other Adaptation, Physiological en_ZA
dc.subject.other Carbon en_ZA
dc.subject.other Phylogeny en_ZA
dc.title Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes en_ZA
dc.type Journal Article en_ZA
dc.rights.holder 2009 Bryan et al; licensee BioMed Central Ltd. en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Engineering & the Built Environment en_ZA
dc.publisher.department Centre for Bioprocess Engineering Research en_ZA
uct.type.filetype Text
uct.type.filetype Image


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This is an Open Access article distributed under the terms of the Creative Commons Attribution License Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License