Evaluation of Plasmodium falciparum gametocyte detection in different patient material

 

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dc.contributor.author Kast, Katharina en_ZA
dc.contributor.author Berens-Riha, Nicole en_ZA
dc.contributor.author Zeynudin, Ahmed en_ZA
dc.contributor.author Abduselam, Nuredin en_ZA
dc.contributor.author Eshetu, Teferi en_ZA
dc.contributor.author Loscher, Thomas en_ZA
dc.contributor.author Wieser, Andreas en_ZA
dc.contributor.author Shock, Jonathan en_ZA
dc.contributor.author Pritsch, Michael en_ZA
dc.date.accessioned 2015-10-30T09:31:42Z
dc.date.available 2015-10-30T09:31:42Z
dc.date.issued 2013 en_ZA
dc.identifier.citation Kast, K., Berens-Riha, N., Zeynudin, A., Abduselam, N., Eshetu, T., Löscher, T., ... & Pritsch, M. (2013). Evaluation of Plasmodium falciparum gametocyte detection in different patient material. Malaria journal, 12(1), 438. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/14518
dc.identifier.uri http://dx.doi.org/10.1186/1475-2875-12-438
dc.description.abstract BACKGROUND:For future eradication strategies of malaria it is important to control the transmission of gametocytes from humans to the anopheline vector which causes the spread of the disease. Sensitive, non-invasive methods to detect gametocytes under field conditions can play a role in monitoring transmission potential. METHODS: Microscopically Plasmodium falciparum-positive patients from Jimma, Ethiopia donated finger-prick blood, venous blood, saliva, oral mucosa and urine samples that were spotted on filter paper or swabs. All samples were taken and stored under equal, standardized conditions. RNA was extracted from the filter paper and detected by real-time QT-NASBA. Pfs16-mRNA and Pfs25-mRNA were measured with a time to positivity to detect gametocyte specific mRNA in different gametocyte stages. They were compared to 18S-rRNA, which is expressed in all parasite stages. Results were quantified via a known dilution series of artificial RNA copies. RESULTS: Ninety-six samples of 16 uncomplicated malaria patients were investigated. 10 (66.7%) of the slides showed gametocyte densities between 0.3-2.9 gametocytes/mul. For all RNA-targets, molecular detection in blood samples was most sensitive; finger-prick sampling required significantly smaller amounts of blood than venous blood collection. Detection of asexual 18S-rRNA in saliva and urine showed sensitivities of 80 and 67%, respectively. Non-invasive methods to count gametocytes proved insensitive. Pfs16-mRNA was detectable in 20% of urine samples, sensitivities for other materials were lower. Pfs25-mRNA was not detectable in any sample. CONCLUSIONS: The sensitivity of non-invasively collected material such as urine, saliva or mucosa seems unsuitable for the detection of gametocyte-specific mRNA. Sensitivity in asymptomatic carriers might be generally even lower. Finger-prick testing revealed the highest absolute count of RNA copies per muL, especially for Pfs25-mRNA copies. The method proved to be the most effective and should preferably be applied in future transmission control and eradication plans. A rapid test for gametocyte targets would simplify efforts. en_ZA
dc.language.iso eng en_ZA
dc.publisher BioMed Central Ltd en_ZA
dc.rights This is an open access article distributed under the terms of the Creative Commons Attribution License en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_ZA
dc.source Malaria Journal en_ZA
dc.source.uri http://www.malariajournal.com/ en_ZA
dc.subject.other Malaria, transmission control and eradication en_ZA
dc.subject.other Plasmodium falciparum en_ZA
dc.title Evaluation of Plasmodium falciparum gametocyte detection in different patient material en_ZA
dc.type Journal Article en_ZA
dc.rights.holder 2013 Kast et al.; licensee BioMed Central Ltd en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Mathematics and Applied Mathematics en_ZA
uct.type.filetype Text
uct.type.filetype Image


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This is an open access article distributed under the terms of the Creative Commons Attribution License Except where otherwise noted, this item's license is described as This is an open access article distributed under the terms of the Creative Commons Attribution License