dc.contributor.author |
Kast, Katharina
|
en_ZA |
dc.contributor.author |
Berens-Riha, Nicole
|
en_ZA |
dc.contributor.author |
Zeynudin, Ahmed
|
en_ZA |
dc.contributor.author |
Abduselam, Nuredin
|
en_ZA |
dc.contributor.author |
Eshetu, Teferi
|
en_ZA |
dc.contributor.author |
Loscher, Thomas
|
en_ZA |
dc.contributor.author |
Wieser, Andreas
|
en_ZA |
dc.contributor.author |
Shock, Jonathan
|
en_ZA |
dc.contributor.author |
Pritsch, Michael
|
en_ZA |
dc.date.accessioned |
2015-10-30T09:31:42Z |
|
dc.date.available |
2015-10-30T09:31:42Z |
|
dc.date.issued |
2013 |
en_ZA |
dc.identifier.citation |
Kast, K., Berens-Riha, N., Zeynudin, A., Abduselam, N., Eshetu, T., Löscher, T., ... & Pritsch, M. (2013). Evaluation of Plasmodium falciparum gametocyte detection in different patient material. Malaria journal, 12(1), 438. |
en_ZA |
dc.identifier.uri |
http://hdl.handle.net/11427/14518
|
|
dc.identifier.uri |
http://dx.doi.org/10.1186/1475-2875-12-438
|
|
dc.description.abstract |
BACKGROUND:For future eradication strategies of malaria it is important to control the transmission of gametocytes from humans to the anopheline vector which causes the spread of the disease. Sensitive, non-invasive methods to detect gametocytes under field conditions can play a role in monitoring transmission potential. METHODS: Microscopically Plasmodium falciparum-positive patients from Jimma, Ethiopia donated finger-prick blood, venous blood, saliva, oral mucosa and urine samples that were spotted on filter paper or swabs. All samples were taken and stored under equal, standardized conditions. RNA was extracted from the filter paper and detected by real-time QT-NASBA. Pfs16-mRNA and Pfs25-mRNA were measured with a time to positivity to detect gametocyte specific mRNA in different gametocyte stages. They were compared to 18S-rRNA, which is expressed in all parasite stages. Results were quantified via a known dilution series of artificial RNA copies. RESULTS: Ninety-six samples of 16 uncomplicated malaria patients were investigated. 10 (66.7%) of the slides showed gametocyte densities between 0.3-2.9 gametocytes/mul. For all RNA-targets, molecular detection in blood samples was most sensitive; finger-prick sampling required significantly smaller amounts of blood than venous blood collection. Detection of asexual 18S-rRNA in saliva and urine showed sensitivities of 80 and 67%, respectively. Non-invasive methods to count gametocytes proved insensitive. Pfs16-mRNA was detectable in 20% of urine samples, sensitivities for other materials were lower. Pfs25-mRNA was not detectable in any sample. CONCLUSIONS: The sensitivity of non-invasively collected material such as urine, saliva or mucosa seems unsuitable for the detection of gametocyte-specific mRNA. Sensitivity in asymptomatic carriers might be generally even lower. Finger-prick testing revealed the highest absolute count of RNA copies per muL, especially for Pfs25-mRNA copies. The method proved to be the most effective and should preferably be applied in future transmission control and eradication plans. A rapid test for gametocyte targets would simplify efforts. |
en_ZA |
dc.language.iso |
eng |
en_ZA |
dc.publisher |
BioMed Central Ltd |
en_ZA |
dc.rights |
This is an open access article distributed under the terms of the Creative Commons Attribution License |
en_ZA |
dc.rights.uri |
http://creativecommons.org/licenses/by/2.0 |
en_ZA |
dc.source |
Malaria Journal |
en_ZA |
dc.source.uri |
http://www.malariajournal.com/
|
en_ZA |
dc.subject.other |
Malaria, transmission control and eradication |
en_ZA |
dc.subject.other |
Plasmodium falciparum |
en_ZA |
dc.title |
Evaluation of Plasmodium falciparum gametocyte detection in different patient material |
en_ZA |
dc.type |
Journal Article |
en_ZA |
dc.rights.holder |
2013 Kast et al.; licensee BioMed Central Ltd |
en_ZA |
uct.type.publication |
Research |
en_ZA |
uct.type.resource |
Article
|
en_ZA |
dc.publisher.institution |
University of Cape Town |
|
dc.publisher.faculty |
Faculty of Science |
en_ZA |
dc.publisher.department |
Department of Mathematics and Applied Mathematics |
en_ZA |
uct.type.filetype |
Text |
|
uct.type.filetype |
Image |
|
dc.identifier.apacitation |
Kast, K., Berens-Riha, N., Zeynudin, A., Abduselam, N., Eshetu, T., Loscher, T., ... Pritsch, M. (2013). Evaluation of Plasmodium falciparum gametocyte detection in different patient material. <i>Malaria Journal</i>, http://hdl.handle.net/11427/14518 |
en_ZA |
dc.identifier.chicagocitation |
Kast, Katharina, Nicole Berens-Riha, Ahmed Zeynudin, Nuredin Abduselam, Teferi Eshetu, Thomas Loscher, Andreas Wieser, Jonathan Shock, and Michael Pritsch "Evaluation of Plasmodium falciparum gametocyte detection in different patient material." <i>Malaria Journal</i> (2013) http://hdl.handle.net/11427/14518 |
en_ZA |
dc.identifier.vancouvercitation |
Kast K, Berens-Riha N, Zeynudin A, Abduselam N, Eshetu T, Loscher T, et al. Evaluation of Plasmodium falciparum gametocyte detection in different patient material. Malaria Journal. 2013; http://hdl.handle.net/11427/14518. |
en_ZA |
dc.identifier.ris |
TY - Journal Article
AU - Kast, Katharina
AU - Berens-Riha, Nicole
AU - Zeynudin, Ahmed
AU - Abduselam, Nuredin
AU - Eshetu, Teferi
AU - Loscher, Thomas
AU - Wieser, Andreas
AU - Shock, Jonathan
AU - Pritsch, Michael
AB - BACKGROUND:For future eradication strategies of malaria it is important to control the transmission of gametocytes from humans to the anopheline vector which causes the spread of the disease. Sensitive, non-invasive methods to detect gametocytes under field conditions can play a role in monitoring transmission potential. METHODS: Microscopically Plasmodium falciparum-positive patients from Jimma, Ethiopia donated finger-prick blood, venous blood, saliva, oral mucosa and urine samples that were spotted on filter paper or swabs. All samples were taken and stored under equal, standardized conditions. RNA was extracted from the filter paper and detected by real-time QT-NASBA. Pfs16-mRNA and Pfs25-mRNA were measured with a time to positivity to detect gametocyte specific mRNA in different gametocyte stages. They were compared to 18S-rRNA, which is expressed in all parasite stages. Results were quantified via a known dilution series of artificial RNA copies. RESULTS: Ninety-six samples of 16 uncomplicated malaria patients were investigated. 10 (66.7%) of the slides showed gametocyte densities between 0.3-2.9 gametocytes/mul. For all RNA-targets, molecular detection in blood samples was most sensitive; finger-prick sampling required significantly smaller amounts of blood than venous blood collection. Detection of asexual 18S-rRNA in saliva and urine showed sensitivities of 80 and 67%, respectively. Non-invasive methods to count gametocytes proved insensitive. Pfs16-mRNA was detectable in 20% of urine samples, sensitivities for other materials were lower. Pfs25-mRNA was not detectable in any sample. CONCLUSIONS: The sensitivity of non-invasively collected material such as urine, saliva or mucosa seems unsuitable for the detection of gametocyte-specific mRNA. Sensitivity in asymptomatic carriers might be generally even lower. Finger-prick testing revealed the highest absolute count of RNA copies per muL, especially for Pfs25-mRNA copies. The method proved to be the most effective and should preferably be applied in future transmission control and eradication plans. A rapid test for gametocyte targets would simplify efforts.
DA - 2013
DB - OpenUCT
DO - 10.1186/1475-2875-12-438
DP - University of Cape Town
J1 - Malaria Journal
LK - https://open.uct.ac.za
PB - University of Cape Town
PY - 2013
T1 - Evaluation of Plasmodium falciparum gametocyte detection in different patient material
TI - Evaluation of Plasmodium falciparum gametocyte detection in different patient material
UR - http://hdl.handle.net/11427/14518
ER -
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en_ZA |