The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay

 

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dc.contributor.author Habte, Habtom H en_ZA
dc.contributor.author Mall, Anwar S en_ZA
dc.contributor.author de Beer, Corena en_ZA
dc.contributor.author Lotz E, Zoe en_ZA
dc.contributor.author Kahn, Delawir en_ZA
dc.date.accessioned 2015-10-28T07:09:55Z
dc.date.available 2015-10-28T07:09:55Z
dc.date.issued 2006 en_ZA
dc.identifier.citation Habte, H. H., Mall, A. S., De Beer, C., Lotz, Z. E., & Kahn, D. (2006). The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay. Virol Journal, 3(24), 99. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/14489
dc.identifier.uri http://dx.doi.org/10.1186/1743-422X-3-99
dc.description.abstract BACKGROUND: Despite the continuous shedding of HIV infected blood into the oral cavity and the detectable presence of the AIDS virus at a high frequency, human saliva is reported to inhibit oral transmission of HIV through kissing, dental treatment, biting, and aerosolization. The purpose of this study was to purify salivary MUC5B and MUC7 mucins from crude saliva and determine their anti-HIV-1 activities. METHODS: Following Sepharose CL-4B column chromatography and caesium chloride isopycnic density-gradient ultra-centrifugation, the purity and identity of the mucins was determined by SDS-PAGE and Western blotting analysis respectively. Subsequently an HIV-1 inhibition assay was carried out to determine the anti-HIV-1 activity of the crude saliva and purified salivary mucins by incubating them with subtype D HIV-1 prior to infection of the CD4+ CEM SS cells. RESULTS: Western blotting analysis confirmed that the mucin in the void volume is MUC5B and the mucin in the included volume is MUC7. The HIV inhibition assay revealed that both the crude saliva and salivary MUC5B and MUC7 mucins inhibited HIV-1 activity by 100%. CONCLUSION: Although the mechanism of action is not clear the carbohydrate moieties of the salivary mucins may trap or aggregate the virus and prevent host cell entry. en_ZA
dc.language.iso eng en_ZA
dc.publisher BioMed Central Ltd en_ZA
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_ZA
dc.source Virology Journal en_ZA
dc.source.uri http://www.virologyj.com/ en_ZA
dc.subject.other Human saliva, HIV transmission, HIV (Viruses), Oral transmission, HIV (Viruses) Research en_ZA
dc.title The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay en_ZA
dc.type Journal Article en_ZA
dc.rights.holder 2006 Habte et al; licensee BioMed Central Ltd. en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Department of Surgery en_ZA
uct.type.filetype Text
uct.type.filetype Image


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This is an Open Access article distributed under the terms of the Creative Commons Attribution License Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License