The porcine circovirus type 1 capsid gene promoter improves antigen expression and immunogenicity in a HIV-1 plasmid vaccine

 

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dc.contributor.author Tanzer, Fiona en_ZA
dc.contributor.author Shephard, Enid en_ZA
dc.contributor.author Palmer, Kenneth en_ZA
dc.contributor.author Burger, Marieta en_ZA
dc.contributor.author Williamson, Anna-Lise en_ZA
dc.contributor.author Rybicki, Edward en_ZA
dc.date.accessioned 2015-10-28T07:03:46Z
dc.date.available 2015-10-28T07:03:46Z
dc.date.issued 2011 en_ZA
dc.identifier.citation Tanzer, F. L., Shephard, E. G., Palmer, K. E., Burger, M., Williamson, A. L., & Rybicki, E. P. (2011). The porcine circovirus type 1 capsid gene promoter improves antigen expression and immunogenicity in a HIV-1 plasmid vaccine. Virol J, 8(1), 51. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/14467
dc.identifier.uri http://dx.doi.org/10.1186/1743-422X-8-51
dc.description.abstract BACKGROUND:One of the promising avenues for development of vaccines against Human immunodeficiency virus type 1 (HIV-1) and other human pathogens is the use of plasmid-based DNA vaccines. However, relatively large doses of plasmid must be injected for a relatively weak response. We investigated whether genome elements from Porcine circovirus type 1 (PCV-1), an apathogenic small ssDNA-containing virus, had useful expression-enhancing properties that could allow dose-sparing in a plasmid vaccine. RESULTS: The linearised PCV-1 genome inserted 5' of the CMV promoter in the well-characterised HIV-1 plasmid vaccine pTHgrttnC increased expression of the polyantigen up to 2-fold, and elicited 3-fold higher CTL responses in mice at 10-fold lower doses than unmodified pTHgrttnC. The PCV-1 capsid gene promoter (Pcap) alone was equally effective. Enhancing activity was traced to a putative composite host transcription factor binding site and a "Conserved Late Element" transcription-enhancing sequence previously unidentified in circoviruses. CONCLUSIONS: We identified a novel PCV-1 genome-derived enhancer sequence that significantly increased antigen expression from plasmids in in vitro assays, and improved immunogenicity in mice of the HIV-1 subtype C vaccine plasmid, pTHgrttnC. This should allow significant dose sparing of, or increased responses to, this and other plasmid-based vaccines. We also report investigations of the potential of other circovirus-derived sequences to be similarly used. en_ZA
dc.language.iso eng en_ZA
dc.publisher BioMed Central Ltd en_ZA
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_ZA
dc.source Virology Journal en_ZA
dc.source.uri http://www.virologyj.com/ en_ZA
dc.subject.other Beak and feather disease virus en_ZA
dc.subject.other Plasmid-based DNA vaccines en_ZA
dc.title The porcine circovirus type 1 capsid gene promoter improves antigen expression and immunogenicity in a HIV-1 plasmid vaccine en_ZA
dc.type Journal Article en_ZA
dc.rights.holder 2011 Tanzer et al; licensee BioMed Central Ltd. en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
uct.type.filetype Text
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This is an Open Access article distributed under the terms of the Creative Commons Attribution License Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License