Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions

 

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dc.contributor.author van der Walt, Eric en_ZA
dc.contributor.author Palmer, Kenneth en_ZA
dc.contributor.author Martin, Darren en_ZA
dc.contributor.author Rybicki, Edward en_ZA
dc.date.accessioned 2015-10-28T07:03:42Z
dc.date.available 2015-10-28T07:03:42Z
dc.date.issued 2008 en_ZA
dc.identifier.citation van der Walt, E., Palmer, K. E., Martin, D. P., & Rybicki, E. P. (2008). Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions. Virol J, 5(11). en_ZA
dc.identifier.uri http://hdl.handle.net/11427/14464
dc.identifier.uri http://dx.doi.org/10.1186/1743-422X-5-61
dc.description.abstract BACKGROUND: A variety of interactions between up to three different movement proteins (MPs), the coat protein (CP) and genomic DNA mediate the inter- and intra-cellular movement of geminiviruses in the genus Begomovirus. Although movement of viruses in the genus Mastrevirus is less well characterized, direct interactions between a single MP and the CP of these viruses is also clearly involved in both intra- and intercellular trafficking of virus genomic DNA. However, it is currently unknown how specific these MP-CP interactions are, nor how disruption of these interactions might impact on virus viability. RESULTS: Using chimaeric genomes of two strains of Maize streak virus (MSV) we adopted a genetic approach to investigate the gross biological effects of interfering with interactions between virus MP and CP homologues derived from genetically distinct MSV isolates. MP and CP genes were reciprocally exchanged, individually and in pairs, between maize (MSV-Kom)- and Setaria sp. (MSV-Set)-adapted isolates sharing 78% genome-wide sequence identity. All chimaeras were infectious in Zea mays c.v. Jubilee and were characterized in terms of symptomatology and infection efficiency. Compared with their parental viruses, all the chimaeras were attenuated in symptom severity, infection efficiency, and the rate at which symptoms appeared. The exchange of individual MP and CP genes resulted in lower infection efficiency and reduced symptom severity in comparison with exchanges of matched MP-CP pairs. CONCLUSION: Specific interactions between the mastrevirus MP and CP genes themselves and/or their expression products are important determinants of infection efficiency, rate of symptom development and symptom severity. en_ZA
dc.language.iso eng en_ZA
dc.publisher BioMed Central Ltd en_ZA
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_ZA
dc.source Virology Journal en_ZA
dc.source.uri http://www.virologyj.com/ en_ZA
dc.subject.other Maize streak virus en_ZA
dc.subject.other Plant Diseases en_ZA
dc.subject.other Recombination, Genetic en_ZA
dc.title Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions en_ZA
dc.type Journal Article en_ZA
dc.rights.holder 2008 van der Walt et al; licensee BioMed Central Ltd. en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation van der Walt, E., Palmer, K., Martin, D., & Rybicki, E. (2008). Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions. <i>Virology Journal</i>, http://hdl.handle.net/11427/14464 en_ZA
dc.identifier.chicagocitation van der Walt, Eric, Kenneth Palmer, Darren Martin, and Edward Rybicki "Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions." <i>Virology Journal</i> (2008) http://hdl.handle.net/11427/14464 en_ZA
dc.identifier.vancouvercitation van der Walt E, Palmer K, Martin D, Rybicki E. Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions. Virology Journal. 2008; http://hdl.handle.net/11427/14464. en_ZA
dc.identifier.ris TY - Journal Article AU - van der Walt, Eric AU - Palmer, Kenneth AU - Martin, Darren AU - Rybicki, Edward AB - BACKGROUND: A variety of interactions between up to three different movement proteins (MPs), the coat protein (CP) and genomic DNA mediate the inter- and intra-cellular movement of geminiviruses in the genus Begomovirus. Although movement of viruses in the genus Mastrevirus is less well characterized, direct interactions between a single MP and the CP of these viruses is also clearly involved in both intra- and intercellular trafficking of virus genomic DNA. However, it is currently unknown how specific these MP-CP interactions are, nor how disruption of these interactions might impact on virus viability. RESULTS: Using chimaeric genomes of two strains of Maize streak virus (MSV) we adopted a genetic approach to investigate the gross biological effects of interfering with interactions between virus MP and CP homologues derived from genetically distinct MSV isolates. MP and CP genes were reciprocally exchanged, individually and in pairs, between maize (MSV-Kom)- and Setaria sp. (MSV-Set)-adapted isolates sharing 78% genome-wide sequence identity. All chimaeras were infectious in Zea mays c.v. Jubilee and were characterized in terms of symptomatology and infection efficiency. Compared with their parental viruses, all the chimaeras were attenuated in symptom severity, infection efficiency, and the rate at which symptoms appeared. The exchange of individual MP and CP genes resulted in lower infection efficiency and reduced symptom severity in comparison with exchanges of matched MP-CP pairs. CONCLUSION: Specific interactions between the mastrevirus MP and CP genes themselves and/or their expression products are important determinants of infection efficiency, rate of symptom development and symptom severity. DA - 2008 DB - OpenUCT DO - 10.1186/1743-422X-5-61 DP - University of Cape Town J1 - Virology Journal LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions TI - Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions UR - http://hdl.handle.net/11427/14464 ER - en_ZA


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