Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barre syndrome by high-throughput AFLP analysis

 

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dc.contributor.author Godschalk, Peggy en_ZA
dc.contributor.author Bergman, Mathijs en_ZA
dc.contributor.author Gorkink, Raymond en_ZA
dc.contributor.author Simons, Guus en_ZA
dc.contributor.author van den Braak, Nicole en_ZA
dc.contributor.author Lastovica, Albert en_ZA
dc.contributor.author Endtz, Hubert en_ZA
dc.contributor.author Verbrugh, Henri en_ZA
dc.contributor.author van Belkum, Alex en_ZA
dc.date.accessioned 2015-10-12T10:49:04Z
dc.date.available 2015-10-12T10:49:04Z
dc.date.issued 2006 en_ZA
dc.identifier.citation Godschalk, P. C., Bergman, M. P., Gorkink, R. F., Simons, G., Van den Braak, N., Lastovica, A. J., ... & Van Belkum, A. (2006). Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barré syndrome by high-throughput AFLP analysis. BMC microbiology, 6(1), 32. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/14163
dc.identifier.uri http://dx.doi.org/10.1186/1471-2180-6-32
dc.description.abstract BACKGROUND:Campylobacter jejuni is the predominant cause of antecedent infection in post-infectious neuropathies such as the Guillain-Barre (GBS) and Miller Fisher syndromes (MFS). GBS and MFS are probably induced by molecular mimicry between human gangliosides and bacterial lipo-oligosaccharides (LOS). This study describes a new C. jejuni-specific high-throughput AFLP (htAFLP) approach for detection and identification of DNA polymorphism, in general, and of putative GBS/MFS-markers, in particular. RESULTS: We compared 6 different isolates of the "genome strain" NCTC 11168 obtained from different laboratories. HtAFLP analysis generated approximately 3000 markers per stain, 19 of which were polymorphic. The DNA polymorphisms could not be confirmed by PCR-RFLP analysis, suggesting a baseline level of 0.6% AFLP artefacts. Comparison of NCTC 11168 with 4 GBS-associated strains revealed 23 potentially GBS-specific markers, 17 of which were identified by DNA sequencing. A collection of 27 GBS/MFS-associated and 17 enteritis control strains was analyzed with PCR-RFLP tests based on 11 of these markers. We identified 3 markers, located in the LOS biosynthesis genes cj1136, cj1138 and cj1139c, that were significantly associated with GBS (P = 0.024, P = 0.047 and P < 0.001, respectively). HtAFLP analysis of 13 highly clonal South African GBS/MFS-associated and enteritis control strains did not reveal GBS-specific markers. CONCLUSION: This study shows that bacterial GBS markers are limited in number and located in the LOS biosynthesis genes, which corroborates the current consensus that LOS mimicry may be the prime etiologic determinant of GBS. Furthermore, our results demonstrate that htAFLP, with its high reproducibility and resolution, is an effective technique for the detection and subsequent identification of putative bacterial disease markers. en_ZA
dc.language.iso eng en_ZA
dc.publisher BioMed Central Ltd en_ZA
dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_ZA
dc.source BMC Microbiology en_ZA
dc.source.uri http://www.biomedcentral.com/bmcmicrobiol/ en_ZA
dc.subject.other Medical Microbiology en_ZA
dc.title Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barre syndrome by high-throughput AFLP analysis en_ZA
dc.type Journal Article en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Division of Medical Microbiology en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Godschalk, P., Bergman, M., Gorkink, R., Simons, G., van den Braak, N., Lastovica, A., ... van Belkum, A. (2006). Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barre syndrome by high-throughput AFLP analysis. <i>BMC Microbiology</i>, http://hdl.handle.net/11427/14163 en_ZA
dc.identifier.chicagocitation Godschalk, Peggy, Mathijs Bergman, Raymond Gorkink, Guus Simons, Nicole van den Braak, Albert Lastovica, Hubert Endtz, Henri Verbrugh, and Alex van Belkum "Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barre syndrome by high-throughput AFLP analysis." <i>BMC Microbiology</i> (2006) http://hdl.handle.net/11427/14163 en_ZA
dc.identifier.vancouvercitation Godschalk P, Bergman M, Gorkink R, Simons G, van den Braak N, Lastovica A, et al. Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barre syndrome by high-throughput AFLP analysis. BMC Microbiology. 2006; http://hdl.handle.net/11427/14163. en_ZA
dc.identifier.ris TY - Journal Article AU - Godschalk, Peggy AU - Bergman, Mathijs AU - Gorkink, Raymond AU - Simons, Guus AU - van den Braak, Nicole AU - Lastovica, Albert AU - Endtz, Hubert AU - Verbrugh, Henri AU - van Belkum, Alex AB - BACKGROUND:Campylobacter jejuni is the predominant cause of antecedent infection in post-infectious neuropathies such as the Guillain-Barre (GBS) and Miller Fisher syndromes (MFS). GBS and MFS are probably induced by molecular mimicry between human gangliosides and bacterial lipo-oligosaccharides (LOS). This study describes a new C. jejuni-specific high-throughput AFLP (htAFLP) approach for detection and identification of DNA polymorphism, in general, and of putative GBS/MFS-markers, in particular. RESULTS: We compared 6 different isolates of the "genome strain" NCTC 11168 obtained from different laboratories. HtAFLP analysis generated approximately 3000 markers per stain, 19 of which were polymorphic. The DNA polymorphisms could not be confirmed by PCR-RFLP analysis, suggesting a baseline level of 0.6% AFLP artefacts. Comparison of NCTC 11168 with 4 GBS-associated strains revealed 23 potentially GBS-specific markers, 17 of which were identified by DNA sequencing. A collection of 27 GBS/MFS-associated and 17 enteritis control strains was analyzed with PCR-RFLP tests based on 11 of these markers. We identified 3 markers, located in the LOS biosynthesis genes cj1136, cj1138 and cj1139c, that were significantly associated with GBS (P = 0.024, P = 0.047 and P < 0.001, respectively). HtAFLP analysis of 13 highly clonal South African GBS/MFS-associated and enteritis control strains did not reveal GBS-specific markers. CONCLUSION: This study shows that bacterial GBS markers are limited in number and located in the LOS biosynthesis genes, which corroborates the current consensus that LOS mimicry may be the prime etiologic determinant of GBS. Furthermore, our results demonstrate that htAFLP, with its high reproducibility and resolution, is an effective technique for the detection and subsequent identification of putative bacterial disease markers. DA - 2006 DB - OpenUCT DO - 10.1186/1471-2180-6-32 DP - University of Cape Town J1 - BMC Microbiology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2006 T1 - Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barre syndrome by high-throughput AFLP analysis TI - Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barre syndrome by high-throughput AFLP analysis UR - http://hdl.handle.net/11427/14163 ER - en_ZA


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