An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers

 

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dc.contributor.advisor Davids, Lester M en_ZA
dc.contributor.author Nsole Biteghe, Fleury Augustin en_ZA
dc.date.accessioned 2015-09-30T13:43:33Z
dc.date.available 2015-09-30T13:43:33Z
dc.date.issued 2015 en_ZA
dc.identifier.citation Nsole Biteghe, F. 2015. An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/14133
dc.description.abstract Melanoma is a form of skin cancer, arising from epidermal cells of the melanocyte lineage, which undergo a series of transformations and genetic alterations that may give rise to both pigmented and unpigmented melanoma. Melanoma represents 4% of all skin cancers but due to its aggressive nature, it accounts for 80% of death among skin cancer patients. South Africa has a melanoma incidence rate that is second worldwide to only Australia. In melanoma; non-metastatic primary tumours are treated by surgical resection. However, metastatic melanoma is highly resistant to conventional radio and chemotherapy, thus reducing the median life of patient's diagnosis with the metastatic form to about 7-9months. Given the implications of the pigment in failure of chemotherapy, two human metastatic pigmented and unpigmented melanoma cell lines were used to investigate the mechanisms underlying chemo-resistance. During the course of this study, the first aim was to determine the concentration of the chemotherapeutic drug dacarbazine (DTIC) causing fifty percent decrease in melanoma cell viability (LD50), then to investigate the possible synergism of hypericin activated-photodynamic therapy in reducing (HYP-PDT) melanoma cell viability, when combined with chemotherapy. In addition we wanted to assess the morphology and the clonogenic capacity of the melanoma cells, after the different treatments and further investigate the ATP-binding cassette (ABC) transporters (ABCB5/1 & ABCG2) expression profile, before and after chemotherapeutic (DTIC) and combination therapy (DTIC+HYP-PDT) treatments. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Cell Biology en_ZA
dc.title An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers en_ZA
dc.type Master Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Department of Human Biology en_ZA
dc.type.qualificationlevel Masters
dc.type.qualificationname MSc (Med) en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Nsole Biteghe, F. A. (2015). <i>An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Human Biology. Retrieved from http://hdl.handle.net/11427/14133 en_ZA
dc.identifier.chicagocitation Nsole Biteghe, Fleury Augustin. <i>"An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Human Biology, 2015. http://hdl.handle.net/11427/14133 en_ZA
dc.identifier.vancouvercitation Nsole Biteghe FA. An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Human Biology, 2015 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/14133 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Nsole Biteghe, Fleury Augustin AB - Melanoma is a form of skin cancer, arising from epidermal cells of the melanocyte lineage, which undergo a series of transformations and genetic alterations that may give rise to both pigmented and unpigmented melanoma. Melanoma represents 4% of all skin cancers but due to its aggressive nature, it accounts for 80% of death among skin cancer patients. South Africa has a melanoma incidence rate that is second worldwide to only Australia. In melanoma; non-metastatic primary tumours are treated by surgical resection. However, metastatic melanoma is highly resistant to conventional radio and chemotherapy, thus reducing the median life of patient's diagnosis with the metastatic form to about 7-9months. Given the implications of the pigment in failure of chemotherapy, two human metastatic pigmented and unpigmented melanoma cell lines were used to investigate the mechanisms underlying chemo-resistance. During the course of this study, the first aim was to determine the concentration of the chemotherapeutic drug dacarbazine (DTIC) causing fifty percent decrease in melanoma cell viability (LD50), then to investigate the possible synergism of hypericin activated-photodynamic therapy in reducing (HYP-PDT) melanoma cell viability, when combined with chemotherapy. In addition we wanted to assess the morphology and the clonogenic capacity of the melanoma cells, after the different treatments and further investigate the ATP-binding cassette (ABC) transporters (ABCB5/1 & ABCG2) expression profile, before and after chemotherapeutic (DTIC) and combination therapy (DTIC+HYP-PDT) treatments. DA - 2015 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers TI - An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers UR - http://hdl.handle.net/11427/14133 ER - en_ZA


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