L-arginine overproduction in Corynebacterium glutamicum ATCC 13032

Theron, Grant de V

dc.contributor.advisor	Reid, Sharon J	en_ZA
dc.contributor.author	Theron, Grant de V	en_ZA
dc.date.accessioned	2015-01-13T03:59:24Z
dc.date.available	2015-01-13T03:59:24Z
dc.date.issued	2009	en_ZA
dc.identifier.citation	Theron, G. 2009. L-arginine overproduction in Corynebacterium glutamicum ATCC 13032. University of Cape Town.	en_ZA
dc.identifier.uri	http://hdl.handle.net/11427/12122
dc.description	Includes abstract.	en_ZA
dc.description	Includes bibliographical references (p. 155-174).	en_ZA
dc.description.abstract	Corynebacterium glutamicum is widely used for the commercial production of a variety of amino acids, including L-lysine, L-glutamate and L-threonine. With the exception of Larginine, the biosynthesis and regulation of most of these compounds in this bacterium are relatively well characterised in the literature. The research presented here focuses on improving our understanding of the regulation of L-arginine biosynthesis in C. glutamicum. This was performed with the ultimate goal of creating strains capable of producing L-arginine commercially. A novel gene replacement system was initially used for the directed mutation of the Larginine biosynthetic gene cluster in C. glutamicum ATCC 13032. This was met with limited success, however, and the pK19mobsacB vector was thus adopted for further mutagenesis of this region.	en_ZA
dc.language.iso	eng	en_ZA
dc.subject.other	Cell Biology	en_ZA
dc.title	L-arginine overproduction in Corynebacterium glutamicum ATCC 13032	en_ZA
dc.type	Doctoral Thesis
uct.type.publication	Research	en_ZA
uct.type.resource	Thesis	en_ZA
dc.publisher.institution	University of Cape Town
dc.publisher.faculty	Faculty of Science	en_ZA
dc.publisher.department	Department of Molecular and Cell Biology	en_ZA
dc.type.qualificationlevel	Doctoral
dc.type.qualificationname	PhD	en_ZA
uct.type.filetype	Text
uct.type.filetype	Image
dc.identifier.apacitation	Theron, G. d. V. (2009). <i>L-arginine overproduction in Corynebacterium glutamicum ATCC 13032</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/12122	en_ZA
dc.identifier.chicagocitation	Theron, Grant de V. <i>"L-arginine overproduction in Corynebacterium glutamicum ATCC 13032."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2009. http://hdl.handle.net/11427/12122	en_ZA
dc.identifier.vancouvercitation	Theron GdV. L-arginine overproduction in Corynebacterium glutamicum ATCC 13032. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2009 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/12122	en_ZA
dc.identifier.ris	TY - Thesis / Dissertation AU - Theron, Grant de V AB - Corynebacterium glutamicum is widely used for the commercial production of a variety of amino acids, including L-lysine, L-glutamate and L-threonine. With the exception of Larginine, the biosynthesis and regulation of most of these compounds in this bacterium are relatively well characterised in the literature. The research presented here focuses on improving our understanding of the regulation of L-arginine biosynthesis in C. glutamicum. This was performed with the ultimate goal of creating strains capable of producing L-arginine commercially. A novel gene replacement system was initially used for the directed mutation of the Larginine biosynthetic gene cluster in C. glutamicum ATCC 13032. This was met with limited success, however, and the pK19mobsacB vector was thus adopted for further mutagenesis of this region. DA - 2009 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 T1 - L-arginine overproduction in Corynebacterium glutamicum ATCC 13032 TI - L-arginine overproduction in Corynebacterium glutamicum ATCC 13032 UR - http://hdl.handle.net/11427/12122 ER -	en_ZA