Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress

 

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dc.contributor.advisor Reid, Sharon J en_ZA
dc.contributor.advisor Abratt, Valerie Rose en_ZA
dc.contributor.author Nonyane, Molati Albert en_ZA
dc.date.accessioned 2015-01-03T18:14:45Z
dc.date.available 2015-01-03T18:14:45Z
dc.date.issued 2010 en_ZA
dc.identifier.citation Nonyane, M. 2010. Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/11175
dc.description Includes bibliographical references (p. 102-112). en_ZA
dc.description.abstract The diverse microbiota of the human gastrointestinal tract plays a major role in the general health of humans. A number of bacterial strains with distinctive properties have been isolated and used commercially as probiotics in order to harness these health benefits and offer them to unhealthy hosts. There are set criteria that have to be followed before new probiotics can be introduced into the market. Five Bifidobacterium isolates with extremely high sucrase activity were randomly selected from a faecal sample from a healthy donor for further characterisation as potential probiotics with the ability to utilize fructo- oligosaccharide substrates in the gut. Phylogenic identification of the isolates to the species level was carried out using sequences of the 16S rRNA gene, 16S-23S rRNA gene spacer region, the heat shock protein (hsp60) and Elongation factor Tu (tuf). en_ZA
dc.language.iso eng en_ZA
dc.subject.other Cell Biology en_ZA
dc.title Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress en_ZA
dc.type Master Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Masters
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Nonyane, M. A. (2010). <i>Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/11175 en_ZA
dc.identifier.chicagocitation Nonyane, Molati Albert. <i>"Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2010. http://hdl.handle.net/11427/11175 en_ZA
dc.identifier.vancouvercitation Nonyane MA. Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2010 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/11175 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Nonyane, Molati Albert AB - The diverse microbiota of the human gastrointestinal tract plays a major role in the general health of humans. A number of bacterial strains with distinctive properties have been isolated and used commercially as probiotics in order to harness these health benefits and offer them to unhealthy hosts. There are set criteria that have to be followed before new probiotics can be introduced into the market. Five Bifidobacterium isolates with extremely high sucrase activity were randomly selected from a faecal sample from a healthy donor for further characterisation as potential probiotics with the ability to utilize fructo- oligosaccharide substrates in the gut. Phylogenic identification of the isolates to the species level was carried out using sequences of the 16S rRNA gene, 16S-23S rRNA gene spacer region, the heat shock protein (hsp60) and Elongation factor Tu (tuf). DA - 2010 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2010 T1 - Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress TI - Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress UR - http://hdl.handle.net/11427/11175 ER - en_ZA


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