Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis

 

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dc.contributor.advisor Farrant, Jill M en_ZA
dc.contributor.advisor Illing, Nicola en_ZA
dc.contributor.author Ginbot, Zekarias Gebremedhin en_ZA
dc.date.accessioned 2014-12-30T06:42:31Z
dc.date.available 2014-12-30T06:42:31Z
dc.date.issued 2011 en_ZA
dc.identifier.citation Ginbot, Z. 2011. Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/10523
dc.description.abstract Studies on resurrection plants and other anhydrobiotic organisms show expression of Late Embryogenesis Abundant (LEA) proteins associated with desiccation tolerance. However, the precise role of these proteins has not been described. This study was undertaken to investigate expression, structure and function of XhLEA1-4S1 and XhLEA1-1S2, Group 1 LEA proteins from Xerophyta humilis, in order to shed light on their role in desiccation tolerance. Complementary DNA (cDNA) of these XhLEAs were cloned into bacterial expression vectors and the recombinant proteins expressed in E. coli. Antibodies were generated and used in determination of expression conditions and immunolocalization studies. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Cell Biology en_ZA
dc.title Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis en_ZA
dc.type Doctoral Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Doctoral
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Ginbot, Z. G. (2011). <i>Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/10523 en_ZA
dc.identifier.chicagocitation Ginbot, Zekarias Gebremedhin. <i>"Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2011. http://hdl.handle.net/11427/10523 en_ZA
dc.identifier.vancouvercitation Ginbot ZG. Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2011 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/10523 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Ginbot, Zekarias Gebremedhin AB - Studies on resurrection plants and other anhydrobiotic organisms show expression of Late Embryogenesis Abundant (LEA) proteins associated with desiccation tolerance. However, the precise role of these proteins has not been described. This study was undertaken to investigate expression, structure and function of XhLEA1-4S1 and XhLEA1-1S2, Group 1 LEA proteins from Xerophyta humilis, in order to shed light on their role in desiccation tolerance. Complementary DNA (cDNA) of these XhLEAs were cloned into bacterial expression vectors and the recombinant proteins expressed in E. coli. Antibodies were generated and used in determination of expression conditions and immunolocalization studies. DA - 2011 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis TI - Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis UR - http://hdl.handle.net/11427/10523 ER - en_ZA


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