Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis

Ginbot, Zekarias Gebremedhin

dc.contributor.advisor	Farrant, Jill M	en_ZA
dc.contributor.advisor	Illing, Nicola	en_ZA
dc.contributor.author	Ginbot, Zekarias Gebremedhin	en_ZA
dc.date.accessioned	2014-12-30T06:42:31Z
dc.date.available	2014-12-30T06:42:31Z
dc.date.issued	2011	en_ZA
dc.identifier.citation	Ginbot, Z. 2011. Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis. University of Cape Town.	en_ZA
dc.identifier.uri	http://hdl.handle.net/11427/10523
dc.description.abstract	Studies on resurrection plants and other anhydrobiotic organisms show expression of Late Embryogenesis Abundant (LEA) proteins associated with desiccation tolerance. However, the precise role of these proteins has not been described. This study was undertaken to investigate expression, structure and function of XhLEA1-4S1 and XhLEA1-1S2, Group 1 LEA proteins from Xerophyta humilis, in order to shed light on their role in desiccation tolerance. Complementary DNA (cDNA) of these XhLEAs were cloned into bacterial expression vectors and the recombinant proteins expressed in E. coli. Antibodies were generated and used in determination of expression conditions and immunolocalization studies.	en_ZA
dc.language.iso	eng	en_ZA
dc.subject.other	Cell Biology	en_ZA
dc.title	Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis	en_ZA
dc.type	Doctoral Thesis
uct.type.publication	Research	en_ZA
uct.type.resource	Thesis	en_ZA
dc.publisher.institution	University of Cape Town
dc.publisher.faculty	Faculty of Science	en_ZA
dc.publisher.department	Department of Molecular and Cell Biology	en_ZA
dc.type.qualificationlevel	Doctoral
dc.type.qualificationname	PhD	en_ZA
uct.type.filetype	Text
uct.type.filetype	Image
dc.identifier.apacitation	Ginbot, Z. G. (2011). <i>Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/10523	en_ZA
dc.identifier.chicagocitation	Ginbot, Zekarias Gebremedhin. <i>"Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2011. http://hdl.handle.net/11427/10523	en_ZA
dc.identifier.vancouvercitation	Ginbot ZG. Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2011 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/10523	en_ZA
dc.identifier.ris	TY - Thesis / Dissertation AU - Ginbot, Zekarias Gebremedhin AB - Studies on resurrection plants and other anhydrobiotic organisms show expression of Late Embryogenesis Abundant (LEA) proteins associated with desiccation tolerance. However, the precise role of these proteins has not been described. This study was undertaken to investigate expression, structure and function of XhLEA1-4S1 and XhLEA1-1S2, Group 1 LEA proteins from Xerophyta humilis, in order to shed light on their role in desiccation tolerance. Complementary DNA (cDNA) of these XhLEAs were cloned into bacterial expression vectors and the recombinant proteins expressed in E. coli. Antibodies were generated and used in determination of expression conditions and immunolocalization studies. DA - 2011 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis TI - Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis UR - http://hdl.handle.net/11427/10523 ER -	en_ZA