Investigation of XvSap promoters from the resurrection plant, Xerophyta viscosa

 

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dc.contributor.advisor Rafudeen, Mohamed S en_ZA
dc.contributor.author Ellick, Tamaryn Lorean en_ZA
dc.date.accessioned 2014-12-28T20:12:15Z
dc.date.available 2014-12-28T20:12:15Z
dc.date.issued 2012 en_ZA
dc.identifier.citation Ellick, T. 2012. Investigation of XvSap promoters from the resurrection plant, Xerophyta viscosa. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/10452
dc.description Includes bibliographical references. en_ZA
dc.description.abstract The XvPSap1 promoter derived from Xerophyta viscosa has been demonstrated to be stress-inducible during dehydration in transgenic Nicotiana tabacum, black Mexican sweetcorn cells and Zea mays. To improve this promoter, for future applications in crop biotechnology, four shortened promoters, XvSap1D, E, F and G were generated by mutagenesis. The generated promoters had circa 50% reduction in size and contained the 5' proximal and 3' distal regions of the XvPsap1 promoter with the internal region removed. The shortened promoters displayed no significant sequence homology to any other known plant promoter, besides XvPSap1. In addition to the shortened promoters, a newly discovered full length XvPSap2 promoter, showing a 56.41% homology with XvPSap 1 was also assessed in this study. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Cell Biology en_ZA
dc.title Investigation of XvSap promoters from the resurrection plant, Xerophyta viscosa en_ZA
dc.type Thesis / Dissertation en_ZA
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Masters en_ZA
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image


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