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Browsing by Author "Shepherd, Dionne N"

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Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant.

Author: Owor, Betty E ; Martin, Darren P ; Shepherd, Dionne N ; Edema, Richard ; Monjane, Ade´rito L ; Rybicki, Edward P ; Thomson, Jennifer A ; Vasani, Arvind Date: 2007 Maize streak virus (MSV) contributes significantly to the problem of extremely low African maize yields. Whilst a diverse range of MSV and MSV-like viruses are endemic in sub-Saharan Africa and neighbouring islands, only a single group of ... Read more
A highly divergent South African geminivirus species illuminates the ancient evolutionary history of this family

Author: Varsani, Arvind ; Shepherd, Dionne N ; Dent, Kyle ; Monjane, Aderito L ; Rybicki, Edward P ; Martin, Darren P Date: 2009 Background: We have characterised a new highly divergent geminivirus species, Eragrostis curvula streak virus (ECSV), found infecting a hardy perennial South African wild grass. ECSV represents a new genus-level geminivirus lineage, and has ... Read more c b
An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins

Author: Windram, Oliver P ; Weber, Brandon ; Jaffer, Mohamed A ; Rybicki, Edward P ; Shepherd, Dionne N ; Varsani, Arvind Date: 2008 Development of vaccine strategies against human papillomavirus (HPV), which causes cervical cancer, is a priority. We investigated the use of virus-like particles (VLPs) of the most prevalent type, HPV-16, as carriers of foreign proteins. ... Read more
Maize streak virus: an old and complex 'emerging' pathogen

Author: Shepherd, Dionne N ; Martin, Darren P ; Van der Walt, Eric ; Dent, Kyle ; Varsani, Arvind ; Rybicki, Edward P Date: 2010 Maize streak virus (MSV; Genus Mastrevirus, Family Geminiviridae) occurs throughout Africa, where it causes what is probably the most serious viral crop disease on the continent. It is obligately transmitted by as many as six leafhopper species ... Read more
A new African streak virus species from Nigeria

Author: Oluwafemi, Sunday ; Varsani, Arvind ; Monjane, Ade´rito L ; Shepherd, Dionne N ; Owor, Betty E ; Rybicki, Edward P ; Martin, Darren P Date: 2008 The African streak viruses (AfSVs) are a diverse group of mastrevirus species (family Geminiviridae) that infect a wide variety of annual and perennial grass species across the African continent and its nearby Indian Ocean islands. Six AfSV ... Read more
Novel sugarcane streak and sugarcane streak Reunion mastreviruses from southern Africa and La Réunion.

Author: Shepherd, Dionne N ; Varsani, Arvind ; Windram, Oliver P ; Lefeuvre, Pierre ; Monjane, Ade´rito L ; Owor, Betty E ; Martin, Darren P Date: 2008 The sugarcane infecting streak viruses (SISVs) are mastreviruses (Family Geminiviridae) belonging to a group of ‘‘African streak viruses’’ (AfSVs) that includes the economically devastating Maize streak virus (MSV). Although there are three ... Read more
A protocol for the rapid isolation of full geminivirus genomes from dried plant tissue

Author: Shepherd, Dionne N ; Martin, Darren P ; Lefeuvre, Pierre ; Monjane, Aderito L ; Owor, Betty E ; Rybicki, Edward P ; Varsani, Arvind Date: 2008 A high-throughput method of isolating and cloning geminivirus genomes from dried plant material, by combining an Extract-n-AmpTM-based DNA isolation technique with rolling circle amplification (RCA) of viral DNA, is presented. Using this ... Read more c b n d
Recombination, decreased host specificity and increased mobility may have driven the emergence of maize streak virus as an agricultural pathogen.

Author: Varsani, Arvind ; Shepherd, Dionne N ; Monjane, Ade´rito L ; Owor, Betty E ; Erdmann, Julia B ; Rybicki, Edward P ; Peterschmitt, Michel ; Briddon, Rob W ; Markham, Peter G ; Oluwafemi, Sunday ; Windram, Oliver P ; Lefeuvre, Pierre ; Lett, Jean-Michel ; Martin, Darren P Date: 2008 Maize streak virus (MSV; family Geminiviridae, genus Mastrevirus), the causal agent of maize streak disease, ranks amongst the most serious biological threats to food security in subSaharan Africa. Although five distinct MSV strains have been ... Read more
Replicative intermediates of maize streak virus found during leaf development

Author: Erdmann, Julia B ; Shepherd, Dionne N ; Martin, Darren P ; Varsani, Arvind ; Rybicki, Edward P ; Jeske, Holger Date: 2010 Geminiviruses of the genera Begomovirus and Curtovirus utilize three replication modes: complementary-strand replication (CSR), rolling-circle replication (RCR) and recombinationdependent replication (RDR). Using two-dimensional gel ... Read more
Restoration of native folding of single-stranded DNA sequences through reverse mutations: an indication of a new epigenetic mechanism

Author: Shepherd, Dionne N ; Martin, Darren P ; Varsani, Arvind ; Thomson, Jennifer A ; Rybicki, Edward P ; Klump, Horst H Date: 2006 We used in vivo (biological), in silico (computational structure prediction), and in vitro (model sequence folding) analyses of singlestranded DNA sequences to show that nucleic acid folding conservation is the selective principle behind a ... Read more c b n d
Successful application of FTA Classic Card technology and use of bacteriophage phi29 DNA polymerase for large-scale field sampling and cloning of complete maize streak virus genomes

Author: Owor, Betty E ; Shepherd, Dionne N ; Taylor, Nigel J ; Edema, Richard ; Monjane, Aderito L ; Thomson, Jennifer A ; Martin, Darren P ; Varsani, Arvind Date: 2007 Leaf samples from 155 maize streak virus (MSV)-infected maize plants were collected from 155 farmers’ fields in 23 districts in Uganda in May/June 2005 by leaf-pressing infected samples onto FTA® Classic Cards. Viral DNA was successfully ... Read more c b n d
The use of maize streak virus (MSV) replication-associated protein mutants in the development of MSV-resistant plants

Author: Shepherd, Dionne N Date: 2003 Maize streak virus (MSV) is the type member of the Mastrevirus genus of the Geminiviridae. As the causal agent of maize streak disease (MSD), MSV is the most significant pathogen of maize in Africa, resulting in crop yield losses of up to ... Read more

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Browsing by Author "Shepherd, Dionne N"

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