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Browsing by Author "Smit, Martha S"

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Beyond bottlenecks: expression of complementary enzymes and permeabilization of cell membranes to improve performance of CYP153A6 in Escherichia coli whole cell biocatalysis

Author: White, Bronwyn Elizabeth Date: 2022 Cytochrome P450s are a diverse and versatile class of enzymes, able to carry out oxyfunctionalisation of hydrocarbons with exceptional regio- and stereospecificity. They show great promise within the medical and fine chemical industries, and ... Read more
Effect of cell permeability and dehydrogenase expression on octane activation by CYP153A6-based whole cell Escherichia coli catalysts

Author: White, Bronwyn E ; Fenner, Caryn J ; Smit, Martha S ; Harrison, Susan T L Date: 20 Sep 2017 Background: The regeneration of cofactors and the supply of alkane substrate are key considerations for the biocatalytic activation of hydrocarbons by cytochrome P450s. This study focused on the biotransformation of n-octane to 1-octanol using ... Read more
The influence of microbial physiology on biocatalyst activity and efficiency in the terminal hydroxylation of n-octane using Escherichia coli expressing the alkane hydroxylase, CYP153A6

Author: Olaofe, Oluwafemi A ; Fenner, Caryn J ; Gudiminchi, Rama Krishna ; Smit, Martha S ; Harrison, Susan T L Date: 2013 Biocatalyst improvement through molecular and recombinant means should be complemented with efficient process design to facilitate process feasibility and improve process economics. This study focused on understanding the bioprocess limitations ... Read more c b
Whole-cell hydroxylation of n-octane by Escherichia coli strains expressing the CYP153A6 operon

Author: Gudiminchi, Rama Krishna ; Randall, Charlene ; Opperman, Diederik J ; Olaofe, Oluwafemi A ; Harrison, Susan T L ; Albertyn, Jacobus ; Smit, Martha S Date: 2012 CYP153A6 is a well-studied terminal alkane hydroxylase which has previously been expressed in Pseudomonas putida and Escherichia coli by using the pCom8 plasmid. In this study, CYP153A6 was successfully expressed in E. coli BL21(DE3) by cloning ... Read more

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Browsing by Author "Smit, Martha S"

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