OpenUCT Home
→
Browsing by Author

Browsing by Author "Shephard, Enid"

Now showing items 1-20 of 21

Next Page

Abrogation of contaminating RNA activity in HIV-1 Gag VLPs

Author: Valley-Omar, Ziyaad ; Meyers, Ann ; Shephard, Enid ; Williamson, Anna-Lise ; Rybicki, Edward Date: 2011 BACKGROUND: HIV-1 Gag virus like particles (VLPs) used as candidate vaccines are regarded as inert particles as they contain no replicative nucleic acid, although they do encapsidate cellular RNAs. During HIV-1 Gag VLP production in ... Read more c b
Characterisation of fibrinogen and fibrin proteolysis by the neutrophil membrane

Author: Kirsch, Richard Date: 1999 Recent studies have identified a novel 600 kDa neutrophil membrane associated protease which degrades fibrinogen, fibrin and C-reactive protein (CRP) during incubation of these ligands with phorbol 12-myristate 13-acetate (PMA, 5-10 ng/ml) ... Read more
Chimaeric HIV-1 subtype C Gag molecules with large in-frame C-terminal polypeptide fusions form virus-like particles.

Author: Halsey, Richard J ; Tanzer, Fiona L ; Meyers, Ann ; Pillay, Sirika ; Lynch, Alisson ; Shephard, Enid ; Williamson, Anna-Lise ; Rybicki, Edward P Date: 2008 HIV-1 Pr55 Gag virus-like particles (VLPs) are strong immunogens with potential as candidate HIV vaccines. VLP immunogenicity can be broadened by making chimaeric Gag molecules: however, VLPs incorporating polypeptides longer than 200 aa fused ... Read more
Detection of natural infection with Mycobacterium intracellulare in healthy wild-caught Chacma baboons (Papio ursinus) by ESAT-6 and CFP-10 IFN-gamma ELISPOT tests following a tuberculosis outbreak

Author: Chege, Gerald ; Warren, Robin ; van Pittius, Nico ; Burgers, Wendy ; Wilkinson, Robert ; Shephard, Enid ; Williamson, Anna-Lise Date: 2008 BACKGROUND:Both tuberculous and non-tuberculous mycobacteria can cause infection in nonhuman primates (NHP), indicating the existence of potential zoonotic transmission between these animals and visitors to zoos or animal handlers in primate ... Read more c b
The effect of two novel C-type lectins, Ba100 and Ba25, isolated from the venom of the puff adder, Bitis arietans on T lymphocyte proliferative responses

Author: Spearman, Catherine Wendy Nest Date: 2008 Includes abstract. Includes bibliographical references (p. 233-318). Read more
Expression of HIV-1 antigens in plants as potential subunit vaccines

Author: Meyers, Ann ; Chakauya, Ereck ; Shephard, Enid ; Tanzer, Fiona ; Maclean, James ; Lynch, Alisson ; Williamson, Anna-Lise ; Rybicki, Edward Date: 2008 BACKGROUND: Human immunodeficiency virus type 1 (HIV-1) has infected more than 40 million people worldwide, mainly in sub-Saharan Africa. The high prevalence of HIV-1 subtype C in southern Africa necessitates the development of cheap, effective ... Read more c b
The fumonisin B₁-fed rat as a model for liver injury, oval ('progenitor') cell proliferation, and carcinogenesis

Author: Lemmer, Eric Richard Date: 1999 Fumonisin B₁ (FB₁‚ ) is a carcinogenic mycotoxin produced by the fimgus Fusarium moniliforme in maize, and is hepatotoxic and hepatocarcinogenic in rats. The goal of this dissertation was to characterise the FB₁-fed rat as a model for liver ... Read more
HIV-1 sub-type C chimaeric VLPs boost cellular immune responses in mice

Author: Pillay, Sirika ; Shephard, Enid ; Meyers, Ann ; Williamson, Anna-Lise ; Rybicki, Edward P Date: 2010 Several approaches have been explored to eradicate HIV; however, a multigene vaccine appears to be the best option, given their proven potential to elicit broad, effective responses in animal models. The Pr55Gag protein is an excellent vaccine ... Read more c b
A neutral protease of the neutrophil surface : role in the proteolysis of C-reactive protein and fibrinogen

Author: Kelly, Sharon Lesley Date: 1995 Both of the acute phase reactants, C-reactive protein and fibrinogen, as well as neutrophils have been shown to accumulate at sites of tissue injury or inflammation. The association of C-reactive protein with neutrophils and the concomitant ... Read more
Nonalcoholic steatohepatitis (NASH) : animal studies of interactive hepatoxicity

Author: Clarkson, Vivian Date: 2004 The term "Nonalcoholic steatohepatitis" (NASH) describes a form of liver disease indistinguishable from alcoholic liver disease, but present in persons who consume insigniﬁcant amounts of alcohol. The spectrum of non-alcoholic liver disease ... Read more
A novel candidate HIV vaccine vector based on the replication deficient Capripoxvirus, Lumpy skin disease virus (LSDV)

Author: Shen, Yen-Ju ; Shephard, Enid ; Douglass, Nicola ; Johnston, Nicolette ; Adams, Craig ; Williamson, Carolyn ; Williamson, Anna-Lise Date: 2011 BACKGROUND: The Capripoxvirus, Lumpy skin disease virus (LSDV) has a restricted host-range and is being investigated as a novel HIV-1 vaccine vector. LSDV does not complete its replication cycle in non-ruminant hosts. METHODS: The safety of ... Read more c b
An oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in mice

Author: Chin'ombe, Nyasha ; Bourn, William ; Williamson, Anna-Lise ; Shephard, Enid Date: 2009 BACKGROUND:The induction of antigen-specific CD8+ T cell cytokine responses against an attenuated, oral recombinant Salmonella enterica serovar Typhimurium vaccine expressing a green fluorescent protein (GFP) model antigen was investigated. ... Read more c b
The porcine circovirus type 1 capsid gene promoter improves antigen expression and immunogenicity in a HIV-1 plasmid vaccine

Author: Tanzer, Fiona ; Shephard, Enid ; Palmer, Kenneth ; Burger, Marieta ; Williamson, Anna-Lise ; Rybicki, Edward Date: 2011 BACKGROUND:One of the promising avenues for development of vaccines against Human immunodeficiency virus type 1 (HIV-1) and other human pathogens is the use of plasmid-based DNA vaccines. However, relatively large doses of plasmid must be ... Read more c b
Pre-clinical assessment of novel candidate HIV-1 vaccines using the Chacma baboon

Author: Chege, Gerald Kimani Date: 2006 Includes bibliographical references (leaves 176-221). Read more
Priming with a recombinant pantothenate auxotroph of Mycobacterium bovis BCG and boosting with MVA elicits HIV-1 Gag specific CD8+ T cells

Author: Chapman, Rosamund ; Shephard, Enid ; Stutz, Helen ; Douglass, Nicola ; Sambandamurthy, Vasan ; Garcia, Irene ; Ryffel, Bernhard ; Jacobs, William ; Williamson, Anna-Lise Date: 2012 A safe and effective HIV vaccine is required to significantly reduce the number of people becoming infected with HIV each year. In this study wild type Mycobacterium bovis BCG Pasteur and an attenuated pantothenate auxotroph strain (BCGΔ panCD ... Read more c b
Priming with recombinant auxotrophic BCG expressing HIV-1 Gag, RT and Gp120 and boosting with recombinant MVA induces a robust T cell response in mice

Author: Chapman, Rosamund ; Stutz, Helen ; Jr, William Jacobs ; Shephard, Enid ; Williamson, Anna-Lise Date: 2013 In previous studies we have shown that a pantothenate auxotroph of Myocbacterium bovis BCG (BCGΔ panCD ) expressing HIV-1 subtype C Gag induced Gag-specific immune responses in mice and Chacma baboons after prime-boost immunization in combination ... Read more c b
Proteases of the neutrophil membrane represent an alternative fibrinolytic pathway to that mediated by plasmin

Author: Adams, Susan Ann Date: 1996 The cellular components of the blood, which become associated with fibrin through specific cellular adhesive processes, play a significant role in the breakdown of fibrin. Fibrinolysis by elastase and cathepsin G, enzymes present within the ... Read more
RNA transmission and expression from inert HIV candidate vaccine virus-like-particles

Author: Valley-Omar, Ziyaad Date: 2008 HIV-1 Gag virus-like-particles (VLPs) produced in various expression systems are potent stimulators of both cellular and humoral immune responses in animal models. The encapsidation of large concentrations of random cellular RNA species is ... Read more
The role of the cytolytic mediators, granulysin and perforin, in tuberculosis

Author: Semple, Patricia Lynn Date: 2008 Protective immunity against mycobacterial infection requires an effective cytolytic response, in addition to an intact Type l (Th1) cytokine pathway. Natural killer (NK) cells and cytolytic T-cells (CTL) are essential components of protective ... Read more
The use of directed evolution to create a stable and immunogenic recombinant BCG expressing a modified HIV-1 Gag antigen

Author: Chapman, Rosamund ; Bourn, William R ; Shephard, Enid ; Stutz, Helen ; Douglass, Nicola ; Mgwebi, Thandi ; Meyers, Ann ; Chin'ombe, Nyasha ; Williamson, Anna-Lise Date: 2014 Numerous features make Mycobacterium bovis BCG an attractive vaccine vector for HIV. It has a good safety profile, it elicits long-lasting cellular immune responses and in addition manufacturing costs are affordable. Despite these advantages ... Read more c b

Now showing items 1-20 of 21

Next Page

Browse

All of OpenUCT