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Browsing by Author "Rybicki, Edward"

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Abrogation of contaminating RNA activity in HIV-1 Gag VLPs

Author: Valley-Omar, Ziyaad ; Meyers, Ann ; Shephard, Enid ; Williamson, Anna-Lise ; Rybicki, Edward Date: 2011 BACKGROUND: HIV-1 Gag virus like particles (VLPs) used as candidate vaccines are regarded as inert particles as they contain no replicative nucleic acid, although they do encapsidate cellular RNAs. During HIV-1 Gag VLP production in ... Read more c b
Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus

Author: Monjane, Aderito ; Pande, Daniel ; Lakay, Francisco ; Shepherd, Dionne ; van der Walt, Eric ; Lefeuvre, Pierre ; Lett, Jean-Michel ; Varsani, Arvind ; Rybicki, Edward ; Martin, Darren Date: 2012 BACKGROUND: Single-stranded (ss) DNA viruses in the family Geminiviridae are proving to be very useful in real-time evolution studies. The high mutation rate of geminiviruses and other ssDNA viruses is somewhat mysterious in that their DNA ... Read more c b
Development of microalgae as a biopharming platform

Author: Els, Johann Hendrik Date: 2019 Microalgae may be a powerful biopharmaceutical production platform that is still in its infancy of development. The research done in this project tested the feasibility of creating algal cell packs, a novel immobilised microalgae transient ... Read more
Enhancement of plant expression vectors using replication and silencing suppressor elements

Author: Jacobs, Raygaana Date: 2018 Molecular farming is gaining traction as a cost-effective platform to produce recombinant proteins. Further improvements can be made, however, to increase overall yield especially for difficult to express proteins. In this study virus-derived ... Read more
Experimental evidence indicating that mastreviruses probably did not co-diverge with their hosts

Author: Harkins, Gordon ; Delport, Wayne ; Duffy, Siobain ; Wood, Natasha ; Monjane, Aderito ; Owor, Betty ; Donaldson, Lara ; Saumtally, Salem ; Triton, Guy ; Briddon, Rob ; Shepherd, Dionne ; Rybicki, Edward ; Martin, Darren ; Varsani, Arvind Date: 2009 BACKGROUND:Despite the demonstration that geminiviruses, like many other single stranded DNA viruses, are evolving at rates similar to those of RNA viruses, a recent study has suggested that grass-infecting species in the genus Mastrevirus ... Read more c b
Experimental observations of rapid Maize streak virus evolution reveal a strand-specific nucleotide substitution bias

Author: van der Walt, Eric ; Martin, Darren ; Varsani, Arvind ; Polston, Jane ; Rybicki, Edward Date: 2008 BACKGROUND: Recent reports have indicated that single-stranded DNA (ssDNA) viruses in the taxonomic families Geminiviridae, Parvoviridae and Anellovirus may be evolving at rates of ~10-4 substitutions per site per year (subs/site/year). These ... Read more c b
Expression of HIV-1 antigens in plants as potential subunit vaccines

Author: Meyers, Ann ; Chakauya, Ereck ; Shephard, Enid ; Tanzer, Fiona ; Maclean, James ; Lynch, Alisson ; Williamson, Anna-Lise ; Rybicki, Edward Date: 2008 BACKGROUND: Human immunodeficiency virus type 1 (HIV-1) has infected more than 40 million people worldwide, mainly in sub-Saharan Africa. The high prevalence of HIV-1 subtype C in southern Africa necessitates the development of cheap, effective ... Read more c b
Expression of HPV-11 L1 protein in transgenic Arabidopsis thaliana and Nicotiana tabacum

Author: Kohl, Thomas ; Hitzeroth, Inga ; Christensen, Neil ; Rybicki, Edward Date: 2007 BACKGROUND:We have investigated the possibility and feasibility of producing the HPV-11 L1 major capsid protein in transgenic Arabidopsis thaliana ecotype Columbia and Nicotiana tabacum cv. Xanthi as potential sources for an inexpensive subunit ... Read more c b
A highly divergent South African geminivirus species illuminates the ancient evolutionary history of this family

Author: Varsani, Arvind ; Shepherd, Dionne ; Dent, Kyle ; Monjane, Aderito ; Rybicki, Edward ; Martin, Darren Date: 2009 BACKGROUND:We have characterised a new highly divergent geminivirus species, Eragrostis curvula streak virus (ECSV), found infecting a hardy perennial South African wild grass. ECSV represents a new genus-level geminivirus lineage, and has a ... Read more c b
More men than women make mucosal IgA antibodies to Human papillomavirus type 16 (HPV-16) and HPV-18: a study of oral HPV and oral HPV antibodies in a normal healthy population

Author: Marais, Dianne ; Sampson, Candice ; Jeftha, Anthea ; Dhaya, Dherendra ; Passmore, Jo-Ann ; Denny, Lynette ; Rybicki, Edward ; Van Der Walt, Eric ; Stephen, Lawrence ; Williamson, Anna-Lise Date: 2006 BACKGROUND:We have previously shown the high prevalence of oral anti-human papillomavirus type 16 (HPV-16) antibodies in women with HPV-associated cervical neoplasia. It was postulated that the HPV antibodies were initiated after HPV antigenic ... Read more c b
Novel Production of Bovine Papillomavirus Pseudovirions in Tobacco Plants

Author: Pietersen, Inge ; van Zyl, Albertha ; Rybicki, Edward ; Hitzeroth, Inga Date: 28 Nov 2020 Vaccine efficacy requires the production of neutralising antibodies which offer protection against the native virus. The current gold standard for determining the presence of neutralising antibodies is the pseudovirion-based neutralisation ... Read more c b
The porcine circovirus type 1 capsid gene promoter improves antigen expression and immunogenicity in a HIV-1 plasmid vaccine

Author: Tanzer, Fiona ; Shephard, Enid ; Palmer, Kenneth ; Burger, Marieta ; Williamson, Anna-Lise ; Rybicki, Edward Date: 2011 BACKGROUND:One of the promising avenues for development of vaccines against Human immunodeficiency virus type 1 (HIV-1) and other human pathogens is the use of plasmid-based DNA vaccines. However, relatively large doses of plasmid must be ... Read more c b
Recombination hotspots and host susceptibility modulate the adaptive value of recombination during maize streak virus evolution

Author: Monjane, Aderito ; van der Walt, Eric ; Varsani, Arvind ; Rybicki, Edward ; Martin, Darren Date: 2011 BACKGROUND:Maize streak virus -strain A (MSV-A; Genus Mastrevirus, Family Geminiviridae), the maize-adapted strain of MSV that causes maize streak disease throughout sub-Saharan Africa, probably arose between 100 and 200 years ago via homologous ... Read more c b
Setting up a platform for plant-based influenza virus vaccine production in South Africa

Author: Mortimer, Elizabeth ; Maclean, James ; Mbewana, Sandiswa ; Buys, Amelia ; Williamson, Anna-Lise ; Hitzeroth, Inga ; Rybicki, Edward Date: 2012 BACKGROUND:During a global influenza pandemic, the vaccine requirements of developing countries can surpass their supply capabilities, if these exist at all, compelling them to rely on developed countries for stocks that may not be available ... Read more c b
Short-term dynamics of nano- and picoplankton in the southern Benguela upwelling system

Author: Dames, Nicole Rebecca Date: 2022 Wind driven coastal upwelling influences the overall physical and chemical properties of coastal regions, as well as the small phytoplankton and microbial communities responsible for the productivity and biogeochemistry governing many of these ... Read more
Stability studies of HIV-1 Pr55gag virus-like particles made in insect cells after storage in various formulation media

Author: Lynch, Alisson ; Meyers, Ann ; Williamson, Anna-Lise ; Rybicki, Edward Date: 2012 BACKGROUND:HIV-1 Pr55gag virus-like particles (VLPs) expressed by baculovirus in insect cells are considered to be a very promising HIV-1 vaccine candidate, as they have been shown to elicit broad cellular immune responses when tested in ... Read more c b
The design, development and characterization of a self-replicating DNA expression technology

Author: de Moor, Warren Ralph Josephus Date: 2020 High quality T-cell immunogenicity can be an elusive type of immunity to generate and one that is often sought after by virologists, immunologists and cancer researchers alike. When T-cell immunity is generated using current methodologies the ... Read more
The Expression of Shuni Virus Nucleocapsid Protein in Nicotiana benthamiana for Use as a Diagnostic Reagent

Author: Verbeek, Matthew James Robert Date: 2019 Many devastating zoonotic viruses such as West Nile and Rift Valley fever viruses are endemic to South Africa, affecting livestock and ultimately, through their arthropod vectors, also infecting humans. One such zoonotic virus that is of ... Read more
The production of foot-and-mouth disease virus-like particles in the plant Nicotiana benthamiana: a potential candidate vaccine for foot-and-mouth disease

Author: O'Connor, Steven Patrick Date: 2018 Foot and mouth disease virus (FMDV) infects cloven-hoofed animals causing the highly contagious foot and mouth disease. It is spread by contact or through aerosol. The disease is often debilitating for infected animals and can be fatal. Severe ... Read more
Therapeutic immunisation of rabbits with cottontail rabbit papillomavirus (CRPV) virus-like particles (VLP) induces regression of established papillomas

Author: Govan, Vandana ; Rybicki, Edward ; Williamson, Anna-Lise Date: 2008 There is overwhelming evidence that persistent infection with high-risk human papillomaviruses (HR-HPV) is the main risk factor for invasive cancer of the cervix. Due to this global public health burden, two prophylactic HPV L1 virus-like ... Read more c b

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