Browsing by Author "Opperman, Diederik J"

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Crystal structure of a thermostable old yellow enzyme from Thermus scotoductus SA-01

Author: Opperman, Diederik J ; Sewell, Bryan T ; Litthauer, Derek ; Isupov, Mikhail N ; Littlechild, Jennifer A ; Heerden, Esta van Date: 2010 Recent characterization of the chromate reductase (CrS) from the thermophile Thermus scotoductus SA-01 revealed this enzyme to be related to the Old Yellow Enzyme (OYE) family. Here, we report the structure of a thermostable OYE homolog in ... Read more
Whole-cell hydroxylation of n-octane by Escherichia coli strains expressing the CYP153A6 operon

Author: Gudiminchi, Rama Krishna ; Randall, Charlene ; Opperman, Diederik J ; Olaofe, Oluwafemi A ; Harrison, Susan T L ; Albertyn, Jacobus ; Smit, Martha S Date: 2012 CYP153A6 is a well-studied terminal alkane hydroxylase which has previously been expressed in Pseudomonas putida and Escherichia coli by using the pCom8 plasmid. In this study, CYP153A6 was successfully expressed in E. coli BL21(DE3) by cloning ... Read more

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