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Browsing by Author "Olaofe, Oluwafemi A"

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Improving the production of a thermostable amidase through optimising IPTG induction in a highly dense culture of recombinant Escherichia coli.

Author: Olaofe, Oluwafemi A ; Burton, Stephanie G ; Cowan, Don A ; Harrison, Susan T L Date: 2010 The production of a novel thermostable amidase (EC 3.5.1.4) from Geobacillus pallidus RAPc8 using recombinant Escherichia coli BL21 (DE3) was investigated. Volumetric and specific enzyme activities were investigated in relation to inducer ... Read more
The influence of microbial physiology on biocatalyst activity and efficiency in the terminal hydroxylation of n-octane using Escherichia coli expressing the alkane hydroxylase, CYP153A6

Author: Olaofe, Oluwafemi A ; Fenner, Caryn J ; Gudiminchi, Rama Krishna ; Smit, Martha S ; Harrison, Susan T L Date: 2013 Biocatalyst improvement through molecular and recombinant means should be complemented with efficient process design to facilitate process feasibility and improve process economics. This study focused on understanding the bioprocess limitations ... Read more c b
Whole-cell hydroxylation of n-octane by Escherichia coli strains expressing the CYP153A6 operon

Author: Gudiminchi, Rama Krishna ; Randall, Charlene ; Opperman, Diederik J ; Olaofe, Oluwafemi A ; Harrison, Susan T L ; Albertyn, Jacobus ; Smit, Martha S Date: 2012 CYP153A6 is a well-studied terminal alkane hydroxylase which has previously been expressed in Pseudomonas putida and Escherichia coli by using the pCom8 plasmid. In this study, CYP153A6 was successfully expressed in E. coli BL21(DE3) by cloning ... Read more

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Browsing by Author "Olaofe, Oluwafemi A"

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