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Browsing by Author "Douglass, Nicola"

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Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector

Author: van Diepen, Michiel ; Chapman, Rosamund ; Douglass, Nicola ; Whittle, Leah ; Chineka, Nicole ; Galant, Shireen ; Cotchobos, Christian ; Suzuki, Akiko ; Williamson, Anna-Lise Date: 04 Oct 2021 Attenuated vaccine strains of lumpy skin disease virus (LSDV) have become increasingly popular as recombinant vaccine vectors, to target both LSDV, as well as other pathogens, including human infectious agents. Historically, these vaccine ... Read more c b
Assessment of an LSDV-Vectored Vaccine for Heterologous Prime-Boost Immunizations against HIV

Author: Chapman, Ros ; van Diepen, Michiel ; Douglass, Nicola ; Galant, Shireen ; Jaffer, Mohamed ; Margolin, Emmanuel ; Ximba, Phindile ; Hermanus, Tandile ; Moore, Penny L ; Williamson, Anna-Lise Date: 05 Nov 2021 The modest protective effects of the RV144 HIV-1 vaccine trial have prompted the further exploration of improved poxvirus vector systems that can yield better immune responses and protection. In this study, a recombinant lumpy skin disease ... Read more c b
Comparative analysis of avian poxvirus genomes, including a novel poxvirus from lesser flamingos (Phoenicopterus minor), highlights the lack of conservation of the central region

Author: Carulei, Olivia ; Douglass, Nicola ; Williamson, Anna-Lise Date: 06 Dec 2017 Background: Avian poxviruses are important pathogens of both wild and domestic birds. To date, seven isolates from subclades A and B and one from proposed subclade E, have had their genomes completely sequenced. The genomes of these isolates ... Read more
A comparative analysis of cowpox virus (CPV WT) and a deletion mutant lacking the gene encoding the inflammation modulatory protein (CPV IMP)

Author: Paulsen, Janis Date: 2007 Cowpox virus has been found to encode the inflammation modulatory protein (IMP) (Miller, C.G., 1997), a homologue vaccinia virus complement control protein (VCP). VCP belongs to regulation of complement activation (RCA) protein superfamily. ... Read more
Comparison of the two lumpy skin disease virus vaccines, Neethling and Herbivac, and construction of a recombinant Herbivac-Rift Valley fever virus vaccine

Author: Omar, Ruzaiq Date: 2015 There are two broad aims to this project. The first aim is to compare and characterise two lumpy skin disease virus (LSDV) vaccines namely the vaccine based on attenuated Neethling LSDV (nLSDV) and HerbivacÂ®LS (Herbivac). The second aim is ... Read more
The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF)

Author: Douglass, Nicola ; Omar, Ruzaiq ; Munyanduki, Henry ; Suzuki, Akiko ; de Moor, Warren ; Mutowembwa, Paidamwoyo ; Pretorius, Alri ; Nefefe, Tshifhiwa ; Schalkwyk, Antoinette van ; Kara, Pravesh ; Heath, Livio ; Williamson, Anna-Lise Date: 20 Oct 2021 Dual vaccines (<i>n</i> = 6) against both lumpy skin disease (LSD) and bovine ephemeral fever (BEF) were constructed, based on the BEFV glycoprotein (G) gene, with or without the BEFV matrix (M) protein gene, inserted into one of two different ... Read more c b
The development of penguinpox virus (PEPV) as a vaccine vector : transfer vector construction and rescue of virus growth in rabbit kidney cells (RK-13) by vaccinia virus K1

Author: Hu, Nai-Chung Date: 2010 Of the many vaccine trials which have taken place, the most promising results have been obtained from the recent phase 3 clinical trial which tested the ability of a dual protein and Canarypox virus recombinant to protect humans against HIV-1 ... Read more
Genetic and phenotypic analysis of novel South African Avian poxviruses

Author: Carulei, Olivia Date: 2018 Avian poxviruses are important pathogens of both wild and domestic birds and exhibit a large degree of intragenus diversity at a genomic level. These viruses are known to differ in growth characteristics (in vitro and in vivo), virulence, and ... Read more
HIV-1 subtype C mosaic Gag expressed by BCG and MVA elicits persistent effector t cell responses in a prime-boost regimen in mice

Author: Jongwe, Tsungai Ivai ; Chapman, Ros ; Douglass, Nicola ; Chetty, Shivan ; Chege, Gerald ; Williamson, Anna-Lise Date: 2016 Over 90% of HIV/AIDS positive individuals in sub-Saharan Africa are infected with highly heterogeneous HIV-1 subtype C (HIV-1C) viruses. One of the best ways to reduce the burden of this disease is the development of an affordable and effective ... Read more c b
Improvement of the Capripoxvirus, lumpy skin disease virus for use as a vaccine vector

Author: Munyanduki, Henry Munyaradzi Date: 2018 Lumpy skin disease (LSD) is a notifiable viral infection due both to its morbidity in cattle and its severe economic burden. The disease was confined to Sub-Saharan Africa but has in recent years spread to the Middle East and Europe. Vaccination ... Read more
Influence of the Viral Superoxide Dismutase (SOD) Homologue on Lumpy Skin Disease Virus (LSDV) Growth, Histopathology and Pathogenicity

Author: Douglass, Nicola ; Munyanduki, Henry ; Omar, Ruzaiq ; Gers, Sophette ; Mutowembwa, Paidamwoyo ; Heath, Livio ; Williamson, Anna-Lise Date: 07 Nov 2020 Lumpy skin disease is an important economic disease of cattle that is controlled by vaccination. This paper presents an investigation into the role of the lumpy skin disease virus (LSDV) superoxide dismutase (SOD) homologue on growth and ... Read more c b
An investigation into the Use of Lumpy Skin Disease Virus as a Vaccine Vector for a Potential HIV-1 vaccine

Author: Shen, Yen-Ju Date: 2010 Read more
Investigation of local South African avipoxviruses as potential vaccine vectors

Author: Offerman, Kristy-Maree Date: 2014 Avipoxviruses are large, genetically diverse DNA viruses which are particularly desirable for use as vaccine vectors as a result of their excellent safety profile and host range restriction. In this study, 8 novel South African (SA) avipoxvirus ... Read more
A novel candidate HIV vaccine vector based on the replication deficient Capripoxvirus, Lumpy skin disease virus (LSDV)

Author: Shen, Yen-Ju ; Shephard, Enid ; Douglass, Nicola ; Johnston, Nicolette ; Adams, Craig ; Williamson, Carolyn ; Williamson, Anna-Lise Date: 2011 BACKGROUND: The Capripoxvirus, Lumpy skin disease virus (LSDV) has a restricted host-range and is being investigated as a novel HIV-1 vaccine vector. LSDV does not complete its replication cycle in non-ruminant hosts. METHODS: The safety of ... Read more c b
Phylogenetic analysis of three genes of Penguinpox virus corresponding to Vaccinia virus G8R (VLTF-1), A3L (P4b) and H3L reveals that it is most closely related to Turkeypox virus, Ostrichpox virus and Pigeonpox virus

Author: Carulei, Olivia ; Douglass, Nicola ; Williamson, Anna-Lise Date: 2009 Phylogenetic analysis of three genes of Penguinpox virus, a novel Avipoxvirus isolated from African penguins, reveals its relationship to other poxviruses. The genes corresponding to Vaccinia virus G8R (VLTF-1), A3L (P4b) and H3L were sequenced ... Read more c b
Priming with a recombinant pantothenate auxotroph of Mycobacterium bovis BCG and boosting with MVA elicits HIV-1 Gag specific CD8+ T cells

Author: Chapman, Rosamund ; Shephard, Enid ; Stutz, Helen ; Douglass, Nicola ; Sambandamurthy, Vasan ; Garcia, Irene ; Ryffel, Bernhard ; Jacobs, William ; Williamson, Anna-Lise Date: 2012 A safe and effective HIV vaccine is required to significantly reduce the number of people becoming infected with HIV each year. In this study wild type Mycobacterium bovis BCG Pasteur and an attenuated pantothenate auxotroph strain (BCGΔ panCD ... Read more c b
Six host-range restricted poxviruses from three genera induce distinct gene expression profiles in an in vivo mouse model

Author: Offerman, Kristy ; Deffur, Armin ; Carulei, Olivia ; Wilkinson, Robert ; Douglass, Nicola ; Williamson, Anna Lise Date: 2015 BACKGROUND: Host-range restricted poxviruses make promising vaccine vectors due to their safety profile and immunogenicity. An understanding of the host innate immune responses produced by different poxvirus vectors would aid in the assessment, ... Read more c b
The use of directed evolution to create a stable and immunogenic recombinant BCG expressing a modified HIV-1 Gag antigen

Author: Chapman, Rosamund ; Bourn, William R ; Shephard, Enid ; Stutz, Helen ; Douglass, Nicola ; Mgwebi, Thandi ; Meyers, Ann ; Chin'ombe, Nyasha ; Williamson, Anna-Lise Date: 2014 Numerous features make Mycobacterium bovis BCG an attractive vaccine vector for HIV. It has a good safety profile, it elicits long-lasting cellular immune responses and in addition manufacturing costs are affordable. Despite these advantages ... Read more c b

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Browsing by Author "Douglass, Nicola"

Browsing by Author "Douglass, Nicola"

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