Browsing by Author "Smith, Elizabeth M"

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    An experimental approach to determine the binding mode of yeast linker histone, Hho1p
    (2003) Smith, Elizabeth M; Patterton, Hugh
    In this study, we have developed an assay to investigate of the binding mode of yeast linker histone Hholp to nucleosomes, using magnetic bead pull-down assays. To understand the structural and regulatory role of, Hholp, the linker histone in Saccharomyces cerevisiae, and that of higher eukaryotes (Thoma et al., 1979), it is essential to determine the manner in which these histones associate with chromatin. It was previously proposed that Hh01p may bind to two nucleosomes simultaneously, where globular domain one and globular domain two bind to the adjacent nucleosomes.
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    Spatial and temporal regulation of IL4Rα expression
    (2008) Smith, Elizabeth M; Brombacher, Frank; Horsnell, William
    In this study, we generated a new mouse model, which allows both inducible and cell-specific deletion and reconstitution of IL-IL4Rα expression. This model has the potential to add a new dimension to our understanding of IL4Rα biology. This has been achieved by using the established Tet System (Goosen and Bujard, 1992) where the crossing of two complementary transgenic mouse lines enable the generation of the final double transgenic model. The first line expresses the transactivator, tTA, from the Tet-Off expression cassette driven by the Vav hemapoeitic specific promoter (Wiesner et al., 2005).