Browsing by Author "Salie, S"

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    Characterization of XV7 and XV9 cDNA clones which confer osmotolerance to Eschericia coli
    (1998) Salie, S; Dakora, Felix D
    The sequence similarity between XV7 and the mammalian syntaxin-like protein kinase receptor shows that XV7 may function similar to that of animal protein kinases. The syntaxin-like protein kinase receptor was recently entered into the Genbank (Thoreau, unpublished), therefore not much is known about its characteristics. It does show similarity to a myotonin protein kinase, MtPK (Thoreau, unpublished). The plant receptor-like protein kinases (RLKs) are structurally related to the polypeptide growth factor receptors of animals (Walker, 1994). Although several ABA and stressresponsive gene have been characterised, their physiological role in the initial perception and transduction of stress signals is not well defined Recent reports suggest that characterising phosphorylation/ dephosphorylation responses and investigation of stress-responsive protein kinases may contribute towards identifying the pivotal regulatory steps in plant responses to environmental stress.
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    The symbiotic interaction of Bradyrhizobium japonicum with bambara groundnut and cowpea and the effects of NOD gene-inducers, daidzein and genistein
    (1998) Salie, S; Dakora, Felix D
    The aim of this project was to investigate whether nodulation, and nitrogen concentration of legumes can be increased by providing additional nod geneinducer compounds. Both daidzein and genistein are nod gene-inducers for rhizobia nodulating cowpea, bambara groundnut, soybean and the common bean.
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    Transfusion practices among children undergoing cardiac surgery admitted to the Red Cross War Memorial Children's Hospital Paediatrics Intensive Care Unit
    (2018) Fitzwanga, Kaiser; Salie, S; Argent, A; Morrow, B
    Objective- We aimed to describe the use of blood products following cardiac surgery, as well as the outcomes and factors associated with post-operative blood product use Design- Prospective, single centre observational study Setting- Paediatric intensive care unit (PICU) in Cape Town, South Africa Patients- One hundred and twenty-six children <18 years old admitted to the PICU following cardiac surgery between July 2017 and January 2018 Interventions- None Measurements and Main Results- The data was prospectively obtained from blood bank charts, intraoperative and PICU observation charts. Demographic data, intraoperative details and post-operative blood product use were extracted from patient records and entered in a standardised case record form. Fifty three percent of children received blood products following cardiac surgery. The blood products transfused included cryoprecipitate (30.9%), packed red cells (22.2%), albumin (18.3%), fresh frozen plasma FFP (15.9%) and platelet concentrate (15.1%). Low haemoglobin level was commonest indication (86%) for red cell use. Bleeding was the commonest indication for FFP (70%) and cryoprecipitate (67%) use. Thrombocytopenia was the commonest indication (84%) for platelet use while hypotension episodes were predominant (95%) in those who received albumin. The standardized mortality ratio was 3.1 vs 0, respectively, among transfused versus non-transfused patients (p<0.0001). The median (IQR) duration of PICU stay was 5 (3-11) vs 2 (2-5) days, respectively in those transfused versus non-transfused (p<0.0001). The median (IQR) ventilation duration was 47(22-132) hours vs 20 (6-27) hours, respectively among the transfused versus non-transfused (p=<0.0001). The factors associated with blood-product use post cardiac surgery include previous cardiac surgery, younger age, lower weights, and prolonged coagulation parameters (p=<0.05). Conclusion- There is high usage of blood products among children post cardiac surgery. The children transfused had a longer ICU stay, ventilation duration, and higher standardized mortality ratio compared to the non-transfused.