Browsing by Author "Pietersen, Gerhard"

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    Isolation, identification and characterisation of three viruses from Pisum sativum in South Africa
    (2000) Jooste, A E C; Pietersen, Gerhard; Rybicki, Ed
    Three virus isolates from pea plants (Pisum sativum) from different regions in South Africa were identified and are discussed in this thesis. A number of techniques were used to identify the viruses including serology, electron microscopy, cytopathological studies, aphid transmission tests, host range studies and molecular-based techniques such as PCR, cloning and sequencing and sequence comparisons.
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    Strain differentiation and screening for resistance against isolates of soybean mosaic virus
    (2001) Pillay, Ravineethum; Pietersen, Gerhard
    The first part of this project attempted to develop a method for the specific detection of the SMV G1 strain. The techniques were based on nucleotide sequence differences in that part of the SMV genome that spans the mid-region of the coat protein gene to the end of the non-coding region (NCR), and in the CI protein-coding region of the genome.