Browsing by Author "O'Riain, M Justin"

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    Aspects of the spatial and behavioural ecology of Hippopotamus amphibious in the Saint Lucia Estuary, KwaZulu-Natal, South Africa
    (2016) Prinsloo, Alexa Simone; Pillay, Deena; O'Riain, M Justin
    The common hippopotamus (Hippopotamus amphibius) is endemic to Africa but threatened by range restriction, exploitation and competition with humans and domestic livestock for access to critical resources such as fresh water and grazing. Collectively these impacts have resulted in population declines throughout most of their range with the consequence that hippos are classified as Vulnerable on the IUCN Red list. Despite these threats to their persistence and their important ecological role as ecosystem engineers there have been surprisingly few studies concerning factors that influence their current distribution and habitat use. Such research is important for developing effective conservation and management plans to improve the protection of vulnerable and ecologically important species such as hippo. South Africa is home to one of a few growing hippo populations and one of the last persisting in an estuarine habitat. The St Lucia Estuary, one of three Ramsar Wetlands of International Importance located in the iSimangaliso Wetland Park (itself a UNESCO World Heritage site), in northern KwaZulu-Natal is not only the largest estuarine system in Africa, but is also home to one of South Africa's largest hippo populations. In this study I explore aspects of the spatial and behavioural ecology of hippo within the St Lucia Estuary. More specifically, I investigate select abiotic and biotic predictors of hippo presence and the frequency with which they return to their diurnal lie-up sites. I used binary logistic regression models to evaluate the effects of the selected habitat features on hippo occurrence at two spatial scales (broad and fine). I used a negative binomial with log-link model to evaluate the effects of the selected habitat features on the frequency of use of sites (hippo persistence) in the fine-scale study. In addition, I employed both instantaneous scan sampling and continuous sampling methods in an attempt to determine the diurnal activity budgets and social interactions of a single group near the mouth of the St Lucia Estuary. I used non-parametric tests to evaluate differences in behaviour with daytime and across sampling days with a view to an improved understanding of how the environment influences the behaviour of hippo in an environment subject to ongoing human modification.
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    Behavioural and isotope ecology of marine-foraging chacma baboons (Papio ursinus) on the Cape Peninsula, South Africa
    (2015) Lewis, Matthew Charles; O'Riain, M Justin; West, Adam
    The dominant vegetation type on the Cape Peninsula, South Africa, is an oligotrophic shrub land that supports low numbers of medium-sized and large terrestrial mammals. Of these, only the adaptable and dextrous chacma baboon (Papio ursinus) has learnt to supplement its diet with protein-rich foods from the marine intertidal zone. There are qualitative descriptions of this behaviour in the literature, but the relative contribution of marine foods to baboon diet, the influence of lunar cycles on exploitation and the impacts of marine foraging on ranging behaviour and activity budgets through different seasons have yet to be quantified. Furthermore, all previous studies included data from troops that had access to nutrient-rich exotic foods, which may have reduced their reliance on marine organisms. For this thesis I collected behavioural data on ranging patterns, activity budgets and diet of a free-ranging, natural-foraging troop through full lunar tidal cycles over consecutive seasons. I supplemented the behavioural data with estimates of the troop's diet composition modelled from stable isotope ratios of foods, faeces and hair samples. The troop ranged over 45.262km2 and travelled an average of 6.044 km per day over the study period. The troop's activity budget was dominated by walking and feeding behaviour, both of which peaked during the hot, dry summer months. Both spatial and behavioural data suggest that the study troop is nutrient-stressed relative to other troops in this population, and hence it was surprising that they only consumed small amounts of marine foods during all four seasons. Models incorporating δ13C and δ15N values of baboon faeces and hair confirmed that marine foods were not major dietary items for these baboons, whilst generalized additive models revealed that a range of abiotic factors negatively affect the exploitation of marine foods. Both the probability and intensity of marine foraging within a given hour declined with increasing tide height and swell height, and fluctuated depending on wind speed and direction. Intensity of marine foraging varied through seasons (it was highest in autumn and lowest in spring), and was higher on the east coast than on the west. Together, these results suggest that exploitation of nutrient-rich foods in the intertidal zone is limited by rapid, unpredictable changes inaccessibility. The levels of deviance left unexplained by these models however imply that other as yet unknown factors (e.g. alkaloids in mussels and limpets) may also limit the troop's exploitation of marine foods. In conclusion, this thesis represents the first in-depth study of marine foraging, a behaviour which exemplifies baboons' remarkable behavioural and dietary flexibility. That said, the temporal unpredictability of ease of access, and potential dangers associated with harvesting this resource, appear to limit how much of this high nutrient food resource baboons are able utilise.
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    Conservation ecology of the Cape clawless otter, Aonyx capensis, in an urban environment
    (2017) Okes, Nicola Catherine; O'Riain, M Justin
    Coastal cities have impacted negatively on freshwater and marine ecosystems - primarily through habitat loss, fragmentation and pollution. Globally, it has been found that otter's dependence on these ecosystems exposes them to a myriad of threats, including loss of habitat, human-wildlife conflict and the bioaccumulation of toxic pollutants. The Cape clawless otter, Aonyx capensis, is the most widely distributed otter species in southern Africa and persists in human-modified habitats, including large cities. The Cape Peninsula provides a unique opportunity to study the impacts of urbanisation on otters as it presents a gradient from densely populated urban areas in the north (City of Cape Town) to sparsely populated areas interspersed with large expanses of natural habitat (Table Mountain National Park) in the south. In this thesis, I investigate the distribution, diet and threats to otters living on the Cape Peninsula. I use sign-based occupancy surveys to determine both broad and fine scale drivers of otter presence within the Peninsula's river systems and predicted that otters would avoid densely populated urban areas and rivers or sections thereof that are heavily transformed and polluted. I collected spraint from living otters and vibrissae from dead otters to investigate their diet. I predicted that otters would show an increased reliance on marine foods in areas where freshwater habitats were degraded in addition to seasonal variation in diet associated with the marked seasonal variation in rainfall and primary productivity typical of temperate Mediterranean ecosystems. I explored both immediate and long-term threats to otters by collating all records of conflict, injury and mortality reported over 5 years in addition to determining PCB levels from road-killed otters. Contrary to my predictions, otters did not avoid urban areas, and were more frequently detected in transformed lowland freshwater river systems close to Marine Protected Areas (MPA). Within rivers otters avoided the relatively pristine, yet unproductive, upper reaches of rivers as well as canalised sections and those with consistently high E.coli counts. I found that otters were feeding on both marine and freshwater prey in both polluted and nonpolluted systems. Where large, transformed lowland wetlands were in close proximity to MPAs, otter diet consisted largely of a combination of freshwater crabs and exotic fish from the polluted systems as well as marine fish and rock lobster from the relatively unpolluted MPA. Isotope results complemented the faecal analyses and confirmed that otters show significant variation in diet between seasons, sites and individuals, suggesting an opportunistic and generalist foraging strategy. Importantly, the dietary results reinforce the distribution model that otters rely heavily on the interface between coastal and lowland wetland and river ecosystems close to the MPA, for both foraging and breeding habitat. However, these are the areas that are transformed and heavily impacted by urban development, and therefore the area where otters would most likely be at risk. I developed a hotspot map of otter conflict across the Peninsula and found that the Peninsula otter population experiences low to moderate levels of conflict throughout most of their current range. High conflict areas are associated with optimal habitat that has been fragmented by canalisation and urban development. Road-killed otters showed signs of accumulation of PCBs in liver tissue suggesting that despite otters being adaptable generalists, their dependence on polluted freshwater systems may have long-term health impacts. Mitigating these threats is possible with improved urban planning, waste water treatment and education of the public. However the success of these approaches requires long-term monitoring which is unlikely to be prioritised by resource constrained conservation authorities. I thus explored whether the large citizen science community in Cape Town can be used to monitor the population. I used Maxent to model otter distribution using citizen reported sightings over 5 years and compared the results with the occupancy model outputs. The predicted Maxent distribution mirrored that provided by occupancy models, and highlighted further areas of suitable otter habitat and routes for dispersal. Together my findings suggest that Cape clawless otters, like many other meso-carnivores in South Africa and globally, display a remarkable ability to adapt to human-modified environments using the interface between degraded freshwater systems and the inshore region to feed on a diverse range of prey. Of concern are the moderate to high levels of conflict with people and dogs, vehicle accidents and the accumulation of toxins. Long-term monitoring of the population and the effect of proposed interventions can be achieved by creating a platform for citizen sightings to be recorded in perpetuity at low cost. This platform can also serve as tool for educating the public on the global challenges of conserving biodiversity within and adjacent to large cities.
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    How attractive is the girl next door? An assessment of spatial mate acquisition and paternity in the solitary Cape dune mole-Rat, Bathyergus suillus
    (Public Library of Science, 2012) Bray, Timothy C; Bloomer, Paulette; O'Riain, M Justin; Bennett, Nigel C
    Behavioural observations of reproduction and mate choice in wild fossorial rodents are extremely limited and consequently indirect methods are typically used to infer mating strategies. We use a combination of morphological, reproductive, spatial, and genetic data to investigate the reproductive strategy of a solitary endemic species, the Cape dune mole-rat Bathyergus suillus. These data provide the first account on the population dynamics of this species. Marked sexual dimorphism was apparent with males being both significantly larger and heavier than females. Of all females sampled 36% had previously reproduced and 12% were pregnant at the time of capture. Post-partum sex ratio was found to be significantly skewed in favour of females. The paternity of fifteen litters (n = 37) was calculated, with sires assigned to progeny using both categorical and full probability methods, and including a distance function. The maximum distance between progeny and a putative sire was determined as 2149 m with males moving between sub-populations. We suggest that above-ground movement should not be ignored in the consideration of mate acquisition behaviour of subterranean mammals. Estimated levels of multiple paternity were shown to be potentially as high as 26%, as determined using sibship and sire assignment methods. Such high levels of multiple paternity have not been found in other solitary mole-rat species. The data therefore suggest polyandry with no evidence as yet for polygyny.
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    In pursuit of a panacea: mitigating human-baboon conflict in the Cape Peninsula, South Africa
    (2013) Kaplan, Bentley; O'Riain, M Justin
    With its unparalleled growth and distribution, the human species is increasingly conflicting with wildlife over crops, livestock and even human life as it infringes on natural habitat. In the Cape Peninsula, South Africa, urbanisation has simultaneously reduced the availability of productive low-lying natural land and provided plentiful high-caloric anthropogenic food items within urban, rural and tourist areas. The chacma baboons that occur in the Cape Peninsula have adapted to exploit this anthropogenic food, bringing them into direct conflict with humans. Despite the use of a costly management strategy (baboon monitors/herders), conflict between the two species persists in the form of property damage and stress to local residents and injury and mortality to baboons. The primary aim of this thesis was to explore new approaches for mitigating human-baboon conflict by experimentally manipulating various facets of the cost-benefit ratio to baboons entering human environments. Initially, I tested the efficacy of three deterrents, namely light prisms, bear bangers and electric fencing in raising the costs of entering human environments and therefore reducing baboon use of those environments. Light prisms were completely ineffective in achieving this end, while bear bangers and electric fencing showed prolonged success with no evidence of habituation. An alternative to raising the cost to baboons of entering human environments (deterrents) is to increase the relative benefits of remaining in natural areas, through provisioning food. Indeed, by manipulating the despotic leadership present in chacma baboons through a feeding patch, I was able to affect a shift in a troop's ranging pattern away from human environments. However, this shift was only significant where access to food in the human environment was restricted. Guided by this finding, I then tested the efficacy of baboon-proof waste bins in excluding baboon access and thereby reducing the benefit of human environments. While the bins were highly effective, their success was contingent on proper use and cooperation by residents. In this vein – to aid collaborative management of the baboon population – I conducted a survey of Cape Peninsula residents concerning various aspects of human-baboon conflict. Primarily, residents indicated a considerable tolerance for baboons despite the generally negative baboon-experience profiles. Further, residents had a poor understanding of baboon management structures as well as baboon conservation status, a finding that requires urgent attention where debates around baboon management are nuanced and emotive. I propose the use of two-way communication platforms (online social networks) that will help to minimise ever-present human-human conflicts. Ultimately, human-wildlife conflict is the combination of constraints imposed by cost, socio-ecological values and animal welfare and arguably it can only be ameliorated through multi-stakeholder engagements; the success of which is contingent on sound scientific data.
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    Landscape requirements of a primate population in a human-dominated environment
    (BioMed Central Ltd, 2012) Hoffman, Tali; O'Riain, M Justin
    INTRODUCTION: As urban and rural land development become widespread features of the global landscape so an understanding of the landscape requirements of displaced and isolated wildlife species becomes increasingly important for conservation planning. In the Cape Peninsula, South Africa, rapid human population growth, and the associated urban and rural land transformation, threatens the sustainability of the local chacma baboon population. Here we analyse spatial data collected from nine of the 12 extant troops to determine their population-level landscape requirements. We use hurdle models to ascertain the key landscape features influencing baboon occurrence and abundance patterns on two hierarchical spatial scales. RESULTS: Both spatial scales produced similar results that were ecologically reliable and interpretable. The models indicated that baboons were more likely to occur, and be more abundant, at low altitudes, on steep slopes and in human-modified habitats. The combination of these landscape variables provides baboons with access to the best quality natural and anthropogenic food sources in close proximity to one another and suitable sleeping sites. Surface water did not emerge as an influential landscape feature presumably as the area is not water stressed. CONCLUSIONS: The model results indicate that land development in the Cape Peninsula has pushed baboons into increasingly marginal natural habitat while simultaneously providing them with predictable and easily accessible food sources in human-modified habitats. The resultant spatial competition between humans and baboons explains the high levels of human-baboon conflict and further erosion of the remaining land fragments is predicted to exacerbate competition. This study demonstrates how the quantification of animal landscape requirements can provide a mechanism for identifying priority conservation areas at the human-wildlife interface.
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    Predictors of white shark Carcharodon carcharias presence at two recreational beaches in a major metropole
    (2017) Loosen, Kristina; O'Riain, M Justin; Kock, Alison A; Winker, Henning
    The presence of white sharks Carcharodon carcharias in False Bay, South Africa follows a clear seasonal cycle. In the austral winter months, white sharks aggregate around Seal Island with female sharks in particular moving to the inshore areas in summer. What triggers these fine scale migrations at one of the largest white shark aggregation sites in the world remains a subject of debate. Previous research identified the environmental variables, water temperature and lunar phase, as significant influences on white shark presence inshore. In this study, I attempt to investigate the influence of prey fish availability on the presence of white sharks at two inshore areas of False Bay, namely Muizenberg and Fish Hoek. In addition, I explored the influence of sea surface temperature (SST), El Niño/Southern Oscillation (ENSO), dolphin presence, diatom patch presence and the occurrence of purse-seine fishing (treknetting) on shark presence. A total of 1209 shark sightings were recorded from 1 January 2006 - 31 December 2015 with 742 sightings at Muizenberg and 467 at Fish Hoek. I used randomization tests to determine whether the occurrence of shark sightings was randomly distributed across the environmental and biological variables and determined the dependencies between the predictor variables. I confirmed that shark sightings were clearly seasonal and sightings peaked between 17.49 - 18.57°C. Fish presence revealed a similar peak at 17.94°C. Randomization tests indicate that a spotter is 66% more likely to detect a shark when fish are present, which supports my prediction of a prey mediated cue. The occurrence of treknetting was also found to be non-randomly linked to white shark presence, increasing the probability to spot a shark to 38% instead of 20% at random. The presence of diatom patches was found to be negatively correlated with SST; with numbers increasing in colder waters. The presence of dolphins, as other predators in False Bay, didn't show a significant pattern with any of the analysed variables. The influence of ENSO on the presence of white sharks was also hypothesized and the data revealed that there was a significant influence of weak negative ENSO values on their inshore occurrence. In addition to providing a better understanding of the ecology of white sharks in False Bay, my results can also be used in shark safety and education programs to reduce the risk of shark human conflict.
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    Residency, habitat use and sexual segregation of white sharks, Carcharodon carcharias in False Bay, South Africa
    (Public Library of Science, 2013) Kock, Alison; O'Riain, M Justin; Mauff, Katya; Meÿer, Michael; Kotze, Deon; Griffiths, Charles
    White sharks ( Carcharodon carcharias ) are threatened apex predators and identification of their critical habitats and how these are used are essential to ensuring improved local and ultimately global white shark protection. In this study we investigated habitat use by white sharks in False Bay, South Africa, using acoustic telemetry. 56 sharks (39 female, 17 male), ranging in size from 1.7-5 m TL, were tagged with acoustic transmitters and monitored on an array of 30 receivers for 975 days. To investigate the effects of season, sex and size on habitat use we used a generalized linear mixed effects model. Tagged sharks were detected in the Bay in all months and across all years, but their use of the Bay varied significantly with the season and the sex of the shark. In autumn and winter males and females aggregated around the Cape fur seal colony at Seal Island, where they fed predominantly on young of the year seals. In spring and summer there was marked sexual segregation, with females frequenting the Inshore areas and males seldom being detected. The shift from the Island in autumn and winter to the Inshore region in spring and summer by females mirrors the seasonal peak in abundance of juvenile seals and of migratory teleost and elasmobranch species respectively. This study provides the first evidence of sexual segregation at a fine spatial scale and demonstrates that sexual segregation in white sharks is not restricted to adults, but is apparent for juveniles and sub-adults too. Overall, the results confirm False Bay as a critical area for white shark conservation as both sexes, across a range of sizes, frequent the Bay on an annual basis. The finding that female sharks aggregate in the Inshore regions when recreational use peaks highlights the need for ongoing shark-human conflict mitigation strategies.
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    Sperm structure and motility in the eusocial naked mole-rat, Heterocephalus glaber: a case of degenerative orthogenesis in the absence of sperm competition?
    (BioMed Central Ltd, 2011) Van der Horst, Gerhard; Maree, Liana; Kotze, Sanet; O'Riain, M Justin
    BACKGROUND:We have studied sperm structure and motility in a eusocial rodent where reproduction is typically restricted to a single male and behaviourally dominant queen. Males rarely compete for access to the queen during her estrus cycle, suggesting little or no role for sperm competition. RESULTS: Our results revealed an atypical mammalian sperm structure with spermatozoa from breeding, subordinate and disperser males being degenerate and almost completely lacking a "mammalian phylogenetic stamp". Sperm structure is characterized by extreme polymorphism with most spermatozoa classified as abnormal. Sperm head shapes include round, oval, elongated, lobed, asymmetrical and amorphous. At the ultrastructural level, the sperm head contains condensed to granular chromatin with large open spaces between the chromatin. Nuclear chromatin seems disorganized since chromatin condensation is irregular and extremely inconsistent. The acrosome forms a cap (ca 35%) over the anterior part of the head. A well defined nuclear fossa and neck with five minor sets of banded protein structures are present. The midpiece is poorly organized and contains only 5 to 7 round to oval mitochondria. The flagellar pattern is 9+9+2. A distinct degenerative feature of the tail principal piece is the absence of the fibrous sheath. Only 7% motile spermatozoa were observed which had exceptionally slow swimming speeds. CONCLUSION: In this species, sperm form has simplified and degenerated in many aspects and represents a specialised form of degenerative orthogenesis at the cellular level.
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    The trophic dynamics of the broadnose sevengill shark (Notorynchus cepedianus) in False Bay, South Africa, using multiple tissue stable isotope analysis
    (2017) De Necker, Leigh; O'Riain, M Justin; Kock, Alison; Lewis, Matthew Charles; Barnett, Adam
    Despite their important ecological role, there is limited quantitative information on the trophic ecology of large, apex predator sharks. This is largely a consequence of their occupying naturally low population densities, being highly mobile and elusive and ranging over large distances. Stable isotopes provide a low cost, non-lethal method for investigating the short and long-term diet of a predator, which when combined with prey data can be used to understand the trophic interactions and the potential regulatory effects they may have on the structure and function of marine ecosystems. In this study, I used non-lethal stable isotope analysis to investigate the trophic dynamics of sevengill sharks, Notorynchus cepedianus, within False Bay, South Africa. A total of 39 muscle biopsies (33 female, six male), and 28 blood plasma samples (25 female, three male) were collected from sevengill sharks. These were analysed together with 161 prey samples from 32 different species, including cephalopods, crustaceans, teleosts, chondrichthyans and marine mammals. In addition, seven white shark, Carcharodon carcharias, muscle samples were collected from False Bay and analysed for comparison with those of sevengills. Sevengills in False Bay had the highest δ15N values of all species sampled in this study (including white sharks) and appear to feed predominantly on a variety of coastal prey species from various functional groups. A stable isotope mixing model revealed that inshore chondrichthyans were their most important prey, with Cape fur seals and inshore teleost species also being important prey groups. There was no apparent seasonal shift in the diet of sevengills, despite clear seasonal aggregation in coastal kelp forests during the summer months. δ15N decreased significantly with sevengill size, with immature females having higher δ15N values than mature females, while δ13C increased with sevengill size. These trends are hypothesised to be linked to dietary shifts associated with the relative use of different habitat types when individuals become sexually mature. There is a need to combine stable isotope data with movement patterns and habitat use to better understand the relationship between isotope ratios and habitat use. Ongoing research on sevengill and white shark movement patterns in False Bay, together with the results presented in this study, will provide important information on the trophic and ecological role that two top predatory sharks play in False Bay. Keywords: Notorynchus cepedianus, sevengill shark, stable isotopes, trophic ecology
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    Understanding the black-backed jackal
    (2017-03) Nattrass, Nicoli; Conradie, Beatrice; Drouilly, Marine; O'Riain, M Justin
    This paper reviews what we know about black-backed jackal ecology, drawing implications for managing human-wildlife conflict with this species. We review the research literature on the black-backed jackals in the context of other African jackal species and with regard to its diet (part 1), its breeding, territoriality and sociality (part 2), and its role as a ‘problem animal’ for small stock farmers (part 3). We argue that both the historical record (see also Nattrass et al., 2017) and the scientific research points to the need to understand the black-backed jackal as a very adaptable animal whose diet, breeding strategies and social arrangements are context-dependent. We draw implications for the management of black-backed jackal predation (part 4) and need for further research, especially on farmlands and landscapes undergoing a transformation in land use. The paper is part of an inter-disciplinary project about sheep farming and predators in the Karoo.
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    Wildlife health in human-modified landscapes: epidemiology of tick-borne pathogens affecting black-backed jackals and caracals
    (2017) Viljoen, Storme; Bishop, Jacqueline M; O'Riain, M Justin
    Despite the importance of disease as a wildlife management challenge, baseline research on the epidemiology of pathogens occurring in wildlife populations within both rural and urban landscapes has received little attention to date. The aim of this study was to improve our understanding of wildlife health in human-modified landscapes in South Africa, by providing comparisons of body condition, host-attached tick diversity and tick-borne pathogen (TBP) epidemiology of two common mesocarnivore species, the black-backed jackal (Canis mesomelas) and caracal (Caracal caracal). Jackals (n=46) and caracals (n=27) were sampled from small livestock farmlands in the Central Karoo region, in addition to caracals from farmlands in Namaqualand (n=14), and the urban matrix of the Cape Peninsula (n=16). Body condition was evaluated using both ratio (Body Mass Index) and residual (Ordinary Least Squares) methods, and morphometry was compared with historical datasets for each species. There was no apparent effect of sex, age class or location on body condition of jackals or caracals. Host-attached tick diversity was highest in urban caracals compared with the two other caracal populations, possibly indicating that they are exposed to a greater diversity of potential tick vectors. Molecular methods (mPCR/RLB; conventional PCR screening and phylogenetic analysis) used to screen for selected pathogens of veterinary and/or zoonotic concern, including Ehrlichia, Anaplasma, Babesia and Theileria species, revealed that Central Karoo jackals exhibited a lower prevalence of TBPs, compared with sympatric caracals. Hepatozoon canis, a ubiquitous pathogen of domestic and wild canids globally, was observed in 46.5% of jackals. Theileria ovis, a piroplasm of small livestock, was found in 4.7% of jackals. Jackals and caracals appear to be distinct in their TBP epidemiological roles, despite sharing similar tick communities. Pathogens found in caracals include Hepatozoon felis, Babesia felis, Babesia leo and a potentially undescribed Babesia species, genetically similar to B. venatorum, an emerging zoonosis. An Anaplasma species previously described in South African domestic dogs was also found in the urban caracals. All caracals were infected with at least one TBP. Together, these findings suggest that land use does not significantly influence the body condition of these adaptable predators, but that there is a health cost associated with living in the urban space. Cape Peninsula caracals show substantially higher rates of TBP co-infection (81% versus 14.8% and 0% in farmlands) and greater pathogen diversity compared to farmland caracals. The findings of this study include numerous examples of previously undescribed genetic diversity of tick-borne pathogens infecting South African mesocarnivores living in transformed landscapes. This work adds to our understanding of wildlife health within the 'One Health' framework and represents the first detailed examination of TBPs in jackals on farmlands and is also the first work that focuses specifically on TPBs in caracals anywhere in the world.