Browsing by Author "Mtintsilana, Asanda"

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    Depression and metabolic syndrome across epidemiological transition
    (2025) Dikoko, Viwe; Dugas, Lara; Mtintsilana, Asanda
    Background: Depression and metabolic syndrome (MetS) are growing global health concerns, but limited research focuses on African-origin populations. This study investigates the association between MetS and depression among individuals in Ghana, South Africa (SA), Jamaica, Seychelles, and the United States (US), emphasizing gender and regional disparities. Methods: Secondary data of 446 participants from the METS (2008-2010) and METS- Microbiome study (2017-2019), a prospective cohort, were analyzed. MetS was defined as meeting at least three of the following criteria: elevated blood pressure, low HDL(High- Density Lipoprotein), elevated triglycerides, or high glucose. Depression was assessed using the CES-D scale (score ≥16 indicating depressive symptoms). Depression was assessed only at follow-up (2019), while MetS was measured at baseline (2010). Logistic regression examined the MetS-depression relationship, stratified by gender, adjusting for demographic and behavioural factors. Findings: The prevalence of MetS was 12% in Ghana (N=23), 17% in SA (N=9), 29% in Jamaica (N=26), 27% in Seychelles (N=33), and 39% in the US (N=36). The prevalence of depression was 15% in Ghana (N=29), 25% in SA (N=13), 18% in Jamaica (N=16), 9% in Seychelles (N=11) and 33% in the US (N=30). No significant overall association between MetS and depression was found. However, in men, individual MetS components showed weak associations at p < 0.1. High blood pressure (OR = 3.46, p<0.1) and low HDL (OR = 3.45, p<0.1) were associated with higher odds of depression, while obesity appeared protective (OR = 0.20, p<0.1). Women showed higher rates of obesity, abdominal adiposity, and depression, particularly in Jamaica, Seychelles, and the US. Age inversely correlated with depression in both genders, with older individuals reporting fewer symptoms. Regionally, living in Ghana, Seychelles, and Jamaica was linked to lower odds of depression compared to the US. Interpretation: This study highlights significant gender and regional differences in the MetS-depression relationship. Women face greater vulnerabilities related to obesity and psychological distress, while men are at higher risk due to elevated blood pressure and low HDL. Tailored public health strategies are needed to address these distinct risks and regional disparities, focusing on mitigating the dual burden of metabolic and mental health challenges. Policy strategies should prioritize integrated screening, targeted education, and community-based services—particularly in low-resource settings—to reduce the dual burden of MetS and depression in populations of African origin.
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    The effect of light on a rat model of depression
    (2014) Mtintsilana, Asanda; Russell, Vivienne A; Dimatelis, Jacqueline J
    Background: Depression is a debilitating mood disorder, negatively affecting an individual’s health and well-being. Despite this, the aetiology of depression remains poorly understood. Consistently, depression treatments are far from satisfactory due to limited efficacy and adverse side effects often associated with them, suggesting a need to improve the current animal models of depression in order to understand the basic mechanisms of the disorder. In an attempt to elucidate the pathophysiology of depression, a rodent model of depression (maternal separation, MS) is used to study the neurobiological mechanisms implicated in depression. However, MS alone produces inconsistent findings and often additional stressors are used to exaggerate the effects of MS. To create a more robust model of MS, MS rats were exposed to chronic constant light (CCL). However, contradictory findings have been reported with CCL. Aims: This study aimed to explore the effects of additional CCL in an MS model by measuring glutamate and potassium-stimulated [3H]DA release in the nucleus accumbens (NAc), testing the effects of CCL on serotonin (5-HT) levels in the hypothalamus and prefrontal cortex (PFC) and measuring ì-opioid receptor (MOR-1) levels in the NAc and orexin receptor (OXR-1 and OXR-2) levels in the PFC. Methods: In order to achieve these aims four experimental groups were chosen, out of which two groups; non-maternally separated (NMS) rats and maternally separated (MS) rats were exposed to CCL for 3 weeks during adolescence and the remaining two groups; NMS and MS rats were not subjected to CCL. At postnatal day 80 (adulthood), rats were decapitated and brain tissue collected for analysis of glutamate- and potassium-stimulated [3H]DA release in the NAc using in vitro superfusion. Serotonin levels in the hypothalamus and PFC were determined using Enzyme-Linked ImmunoSorbent Assay (ELISA). Western blot analysis was used to measure MOR-1 levels in the NAc, OXR-1 and OXR-2 in the PFC. Results: MS caused a significant decrease in glutamate-stimulated [3H]DA release in the NAc. In the NAc shell, CCL exposure revealed a trend towards a decrease in [3H]DA release in response to both glutamate- and potassiumstimulation. Moreover, in the hypothalamus NMS and MS rats subjected to CCL had significantly increased 5-HT levels compared to NMS and MS rats without xvii CCL exposure. In the PFC CCL had a significant effect on 5-HT levels and it was revealed that NMS CCL rats had decreased 5-HT levels compared to NMS rats. Similarly, MS CCL rats had significantly decreased 5-HT levels compared to NMS. MS and CCL did not have any significant effect on MOR-1 protein levels in the NAc. On the other hand, MS rats had increased OXR-1 and OXR-2 proteins levels in the PFC compared to NMS and MS CCL rats. Conclusion: MS decreased glutamate-stimulated [3H]DA release in the NAc. Serotonin levels in the hypothalamus and PFC were altered by the effects of MS and CCL. Furthermore, MS exposure increased OXR-1 and OXR-2 protein levels in the PFC. However, MS and CCL did not alter MOR-1 protein levels in the NAc. Therefore, this study has demonstrated that CCL exaggerated the effects of MS and created a more robust model of MS.