Browsing by Author "Moore, John P"

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    Desiccation-induced ultrastructural and biochemical changes in the leaves of the resurrection plant Myrothamnus flabellifolia
    (CSIRO Publishing, 2007) Moore, John P; Hearshaw, Meredith; Ravenscroft, Neil; Lindsey, George G; Farrant, Jill M; Brandt, Wolf F
    Light microscopy and low-temperature scanning electron microscopy were used to systematically compare the surface and internal ultrastructures of hydrated and desiccated leaves of the resurrection plant Myrothamnus flabellifolia (Welw.). This revealed that leaf tissue underwent considerable shrinkage and collapse on desiccation but was supported by a framework of vascular and sclerenchymous tissue, which is responsible for the fan-like shape of the leaves. In addition, the leaf ribs were covered with wax and an internal wax cuticle was observed. Biochemical analysis showed that the cyanidin 3-glucoside content increased on desiccation as did the trehalose and sucrose contents. Salt deposits were observed at the apices of desiccated leaves in the proximity of hydathode-like structures. We propose that this might regulate the leaf salt content since decreased intracellular cation concentration was observed in desiccated leaves. We believe that these unique adaptations contribute to the remarkable desiccation-tolerance properties of this plant.
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    An overview of the biology of the desiccation-tolerant resurrection plant Myrothamnus flabellifolia.
    (Oxford University Press, 2007) Moore, John P; Lindsey, George G; Farrant, Jill M; Brandt, Wolf F
    Myrothamnus flabellifolia (Welw.) is a relatively large resurrection plant, a woody shrub between 0.5 m and 1.5 m tall (Sherwin et al., 1998) that grows on rock inselbergs (Porembski and Barthlott, 2000) throughout southern Africa (Weimarck, 1936; Van Wyk et al., 1997; Glen et al., 1999). The plant was first recorded in 1859 by Friedrich Welwitsch, who named the plant Myrothamnus (myron meaning aromatic and thamnos meaning bush) flabellifolia (meaning fan-like leaves) (Puff, 1978a; Glen et al., 1999), the leaves having a balsamic-like odour (Puff, 1978a; Glen et al., 1999). Weiss (1906) was the first to note the ‘miraculous manner’ with which the desiccated plant revived when supplied with water (Fig. 1A, B). Myrothamnus flabellifolia occupies an important position in traditional African folklore and medicine (Watt and Breyer-Brandwijk, 1962; Hutchings, 1996; Van Wyk et al., 1997). The Zulu name for the plant is ‘uvukwaba- file’ (wakes from the dead). The reviving ability is believed to be passed on to the ill person during treatment (Hutchings, 1996; Van Wyk et al., 1997). The plant is a geophyte possessing an extensive root system which extends into the crevices of the rocky slopes upon which it grows (Child, 1960; Glen et al., 1999). Myrothamnus flabellifolia can dehydrate its vegetative tissue, in particular its leaves, to an air-dry state. In this state, the leaves and stem segments curl and change colour from green to dullbrown (Farrant et al., 1999; Glen et al., 1999). When water is provided to the roots the plant re-hydrates its desiccated tissue and returns to its original colour and shape (Glen et al., 1999; Farrant et al., 2003). Since the last review on M. flabellifolia was written many years ago (Puff, 1978a) and since considerable work has been published in the last decade, this review focuses on recent advances in the understanding of the physiology, biochemistry and chemistry of M. flabellifolia.
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    The predominant polyphenol in the leaves of the resurrection plant Myrothamnus flabellifolius, 3,4,5 tri-O-galloylquinic acid, protects membranes against desiccation and free radical-induced oxidation
    (Portland Press, 2005) Moore, John P; Westall, Kim L; Ravenscroft, Neil; Farrant, Jill M; Lindsey, George G; Brandt, Wolf F
    The predominant (>90%) low-molecular-mass polyphenol was isolated from the leaves of the resurrection plant Myrothamnus flabellifolius and identified to be 3,4,5 tri-O-galloylquinic acid using 1 H and 13C one- and two-dimensional NMR spectroscopy. The structure was confirmed by mass spectrometric analysis. This compound was present at high concentrations, 44%(by weight) in hydrated leaves and 74% (by weight) in dehydrated leaves. Electron microscopy of leaf material fixed with glutaraldehyde and caffeine demonstrated that the polyphenols were localized in large vacuoles in both hydrated and dehydrated leaves. 3,4,5 Tri-O-galloylquinic acid was shown to stabilize an artificial membrane system, liposomes, against desiccation if the polyphenol concentration was between 1 and 2 µg/µg phospholipid. The phase transition of these liposomes observed at 46 ◦C was markedly diminished by the presence of 3,4,5 tri-O-galloylquinic acid, suggesting that the presence of the polyphenol maintained the membranes in the liquid crystalline phase at physiological temperatures. 3,4,5 Tri-O-galloylquinic acid was also shown to protect linoleic acid against free radical-induced oxidation.
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    Response of the leaf cell wall to desiccation in the resurrection plant Myrothamnus flabellifolius
    (American Society of Plant Biologists, 2006) Moore, John P; Nguema-Ona, Eric; Chevalier, Laurence; Lindsey, George G; Brandt, Wolf F; Lerouge, Patrice; Farrant, Jill M; Driouich, Azeddine
    The Myrothamnus flabellifolius leaf cell wall and its response to desiccation were investigated using electron microscopic, biochemical, and immunocytochemical techniques. Electron microscopy revealed desiccation-induced cell wall folding in the majority of mesophyll and epidermal cells. Thick-walled vascular tissue and sclerenchymous ribs did not fold and supported the surrounding tissue, thereby limiting the extent of leaf shrinkage and allowing leaf morphology to be rapidly regained upon rehydration. Isolated cell walls from hydrated and desiccated M. flabellifolius leaves were fractionated into their constituent polymers and the resulting fractions were analyzed for monosaccharide content. Significant differences between hydrated and desiccated states were observed in the water-soluble buffer extract, pectin fractions, and the arabinogalactan protein-rich extract. A marked increase in galacturonic acid was found in the alkali-insoluble pectic fraction. Xyloglucan structure was analyzed and shown to be of the standard dicotyledonous pattern. Immunocytochemical analysis determined the cellular location of the various epitopes associated with cell wall components, including pectin, xyloglucan, and arabinogalactan proteins, in hydrated and desiccated leaf tissue. The most striking observation was a constitutively present high concentration of arabinose, which was associated with pectin, presumably in the form of arabinan polymers. We propose that the arabinan-rich leaf cell wall of M. flabellifolius possesses the necessary structural properties to be able to undergo repeated periods of desiccation and rehydration.
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    The South African and Namibian to populations of the resurrection plant Myrothamnus flabellifolius are genetically distinct and display variation in their galloylquinic acid composition
    (Springer Verlag, 2005) Moore, John P; Farrant, Jill M; Lindsey, George G; Brandt, Wolf F
    The polyphenol contents and compositions in desiccated leaves of Myrothamnus flabellifolius plants collected in various locations in Namibia and South Africa were analyzed using UV spectroscopy and high-performance liquid chromatography–mass spectrometry. A study of the genetic relatedness of these populations was also performed by determination of the DNA sequence of the intergenic spacer region between the psbA and the trnH genes in the chloroplast genome. Namibian M. flabellifolius plants contained signi- ficantly more polyphenols than South African plants. Namibian plants essentially contained a single polyphenol, 3,4,5-tri-O-galloylquinic acid, whereas South African plants contained a variety of galloylquinic acids including 3,4,5-tri-O-galloylquinic acid together with higher molecular weight galloylquinic acids. Sequence analysis revealed a 1.4% divergence between Namibian and South African plants corresponding to the separation of these populations of approximately 4 106 years. The significance of the polyphenol content and composition to the desiccation tolerance of the two populations is discussed.