Browsing by Author "Matiru, Viviene N"
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- ItemOpen AccessAn assessment of rhizobial infection, metabolite release and growth response in agriculturally important legume and cereal crops(2004) Matiru, Viviene N; Imbuga, MabelReports on the natural and laboratory infection of cereals by rhizobium provided the impetus to embark on research using African landraces of sorghum and millet to study their interaction with rhizobia. Seven strains of root-nodule bacteria (namely Rhizobium GHR2, Bradyrhizobium japonicum Tal 110, Sinorhizobium meliloti strain 1, Rhizobium leguminosarum bv. viceae Cn6, R. leguminosarum bv. viceae strain 30, Rhizobium NGR234 and Azorhizobium caulinodans ORSS71, hereafter referred to as ""rhizobia"") that fix N2, were used to study rhizobial effects on sorghum and millet seedlings grown aseptically in Leonard jars with Yz strength Hoagland nutrient solution containing 1 mM KN03.
- ItemOpen AccessPotential use of rhizobial bacteria as promoters of plant growth for increased yield in landraces of African cereal crops(2004) Matiru, Viviene N; Dakora, Felix DRhizobia form root nodules that fix nitrogen (N2) in symbiotic legumes. Extending the ability of these bacteria to fix N2 in non-legumes such as cereals would be a useful technology for increased crop yields among resource-poor farmers. Although some inoculation attempts have resulted in nodule formation in cereal plants, there was no evidence of N2 fixation. However, because rhizobia naturally produce molecules (auxins, cytokinins, abscicic acids, lumichrome, rhiboflavin, lipo-chito-oligosaccharides and vitamins) that promote plant growth, their colonization and infection of cereal roots would be expected to increase plant development, and grain yield. We have used light, scanning, and transmission electron microscopy to show that roots of sorghum and millet landraces from Africa were easily infected by rhizobial isolates from five unrelated legume genera. With sorghum, in particular, plant growth and phosphorus (P) uptake were significantly increased by rhizobial inoculation, suggesting that field selection of suitable rhizobia/cereal combinations could increase yields and produce fodder for livestock production.