Browsing by Author "Halsey, Richard James"

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    Construction and characterization of chimaeric human immunodefiency virus type 1 subtype C Gag virus-like particles
    (2006) Halsey, Richard James; Tanzer, Fiona; Jaffray, Ann; Rybicki, Ed
    In this study I explored the possibility of making HIV-1 subtype C Pr55gag-based chimaeric virus-like particles (VLPs) as a boost to the HIV-IC multigene DNA vaccine pTHr.grttnC, which encodes a modified Gag-RT-Tat-Nef fusion protein (GRTTN). Furthermore, an attempt was made to produce VLP analogues to the HIV-IA polyepitope DNA vaccine pTHr.HIVA. A range of in-frame fusions with the C-termini of myristylation-competent p6-truncated Gag and native Pr55gag were made to test how the length of polypeptide and its sequence might affect VLP formation and structure.