Browsing by Author "Basic, Dunja"

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    Grazer control on benthic algae in the Berg River, Western Cape, South Africa
    (2011) Basic, Dunja; Ewart-Smith, Justine L; Day, Jenny
    Benthic environments in streams are areas of high biological activity where primary producers and consumers interact with each other and the environment. Various factors such as light, nutrient availability and temperature affect algal biomass accumulation however disturbance factors and grazing lead to biomass losses as well as changes in the species composition and densities of algae. There are little, if any, studies done on the effects of grazers on benthic algae in South African rivers and as such, this experiment was done in the Berg River, in the Western Cape of South Africa. The aim was to ascertain the effects of herbivores on benthic algae within the river and lit was hypothesized that periphyton biomass would be higher where grazer densities were lower and that the differences observed in biomass of periphyton between treatments would increase over time. It was also hypothesized that there would be a lower diversity of algae where there were lower herbivore densities. A randomized block design experiment was set up in the Berg River and treatments of 'excluded herbivores' and 'included herbivores' were set up, with two pairs of each treatment spread across four replicates. Stones from the stream were randomly selected and placed in the boxes. After five days half of the boxes were sampled and after eight days, the remaining half was sampled. Stones were scrubbed to remove periphyton from the stones and a sub-sample of 50ml was removed from each for later algal species identification. Invertebrates were removed from the boxes, preserved in ethanol and later identified to family level. Chl-a and AEDW (mg/m²) were determined from the samples as were invertebrate and algal densities. The results showed that there were no herbivore density effects on biomass most likely due to the season of sampling and the length of the experiment. However, the effect on algal densities was more pronounced as there was a higher variety of algal forms present where herbivore densities were lower. To get a better idea of how herbivores affect periphyton other factors need to be taken into account like experimental time period, season and different environmental conditions.
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    Phosphorous uptake and utilization efficiency in cluster root and non-cluster root forming species of the Core Cape Subregion, South Africa
    (2015) Basic, Dunja; Chimphango, Samson; Muasya, Muthama
    The Core Cape Subregion (CCR) is made up of a mosaic of highly weathered and nutrient leached soil substrates in the Western Cape. Plant available phosphorus (P) in these soils is very low, generally ranging from 0.4-3.7 µg P g-1 soil and as a result plants have evolved a number of traits to enhance P-acquisition, such as increased root surface area (SA) and specific root length (SRL), cluster root and root hair proliferation and exudation of organic acids and acid phosphatases (APase) from the roots. Crop yield is limited worldwide due to the unavailability of P and P-fertilization is showing limited success due to soil retention. Sustainable management of this would include exploiting plants with natural adaptations for enhanced P acquisition and utilization. The aim of this study was to discover whether cluster root forming species are more efficient at P acquisition than non-cluster root species. This was achieved by focusing on two objectives: (1) to characterize root traits for increased P acquisition in different soils of the CCR and (2) comparing P-uptake and utilization efficiencies of cluster root species to non-cluster root species under glasshouse and natural conditions. Plants from Fabaceae, Polygalaceae, Proteaceae, Cyperaceae, and Juncaceae were grown in two different glasshouse experiments and observed in a field study.
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    Phosphorous uptake rate in two low phosphorous updated species, Aspalathus linearis and Podalyria calyptrata
    (2011) Basic, Dunja; Chimphango, Samson B M; Cramer, Michael D
    Due to the low P nature of soils within the fynbos biome of the Cape Floristic Region, plants have developed numerous mechanisms which enable them to better acquire phosphorous. A number of species have been reported to have specialised root morphologies (root clusters, mycorrhizae) that enhance P uptake. Plants may also down-regulate the uptake of P by decreasing the expression of genes that encode P transporters. Two Fabaceae species, Podalyria calyptrata and Aspa/athus linearis, were grown in hydroponics for 5 months at a low P supply of 4μM and P-depletion studies were conducted thereafter at 5 levels of external P (4, 10, 20, 50 and lOOμM). Growth rates (biomass accumulation) were also calculated as were root to shoot ratios for both species. A. linearis had a higher uptake rate than P. calyptrata. While the fresh biomass growth rate was similar in both species, P. calyptrata had a higher dry weight root to shoot ratio than A. linearis. The results showed that both species exhibited a lack of response to increasing P concentrations and had similar RGRs. Their uptake rates differed significantly (p<0.05) and this was likely due to their different root:shoot ratios. This indicates that both species would effectively grow in low P soils and in the case of P. calyptrata, in high P soils as well.