Browsing by Author "Africa, Cindy-Jade"

Now showing 1 - 5 of 5
  • Thumbnail Image
    Item
    Restricted
    Attachment of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum cultured under varying conditions to pyrite, chalcopyrite, low-grade ore and quartz in a packed column reactor
    (Springer Verlag, 2012) Africa, Cindy-Jade; van Hille, Robert P; Harrison, Susan T L
    The attachment of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum spp. grown on ferrous medium or adapted to a pyrite mineral concentrate to four mineral substrata, namely, chalcopyrite and pyrite concentrates, a low-grade chalcopyrite ore (0.5 wt%) and quartzite, was investigated. The quartzite represented a typical gangue mineral and served as a control. The attachment studies were carried out in a novel particle-coated column reactor. The saturated reactor containing glass beads, which were coated with fine mineral concentrates, provided a quantifiable surface area of mineral concentrate and maintained good fluid flow. A. ferrooxidans and Leptospirillum spp. had similar attachment characteristics. Enhanced attachment efficiency occurred with bacteria grown on sulphide minerals relative to those grown on ferrous sulphate in an ore-free environment. Selective attachment to sulphide minerals relative to gangue materials occurred, with mineral adapted cultures attaching to the minerals more efficiently than ferrous grown cultures. Mineral-adapted cultures showed highest levels of attachment to pyrite (74% and 79% attachment for A. ferrooxidans and L. ferriphilum, respectively). This was followed by attachment of mineral-adapted cultures to chalcopyrite (63% and 58% for A. ferrooxidans and L. ferriphilum, respectively). A. ferrooxidans and L. ferriphilum exhibited lower levels of attachment to low-grade ore and quartz relative to the sulphide minerals.
  • Thumbnail Image
    Item
    Restricted
    In situ investigation and visualisation of microbial attachment and colonisation in a heap bioleach environment: the novel biofilm reactor
    (Elsevier, 2010) Africa, Cindy-Jade; Harrison, Susan T L; Becker, Megan; van Hille, Robert P
    In this paper, the development of a novel means of investigating the attachment and subsequent biofilm formation of mineral bioleaching micro-organisms to mineral surfaces in situ is described. The protocol was developed to investigate the interactions of micro-organisms with sulfide minerals and low-grade chalcopyrite ore under conditions resemblant of a bioheap environment. The method makes use of a biofilm reactor in which thin sections of mineral ore are mounted. The reactor is operated as a continuous flow-through system. Attachment of pure and mixed cultures of Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum is assessed. The technique allows for the investigation of microbial ecology with special regard to microbe–mineral attachment, site and mineral specific associations of micro-organisms and spatial organisation of microbial communities present through the use of fluorescent microscopy techniques. Preliminary fluorescent in situ hybridisation (FISH) analysis of the attachment of L. ferriphilum and A. ferrooxidans to massive chalcopyrite sections, as well as to low-grade chalcopyrite containing ore sections is presented. In the case of both low-grade and massive sulfide mineral samples, attachment of mixed micro-colonies was observed in regions where surface defects were prevalent. In low-grade samples, preferential attachment was observed in regions where sulfide minerals were present. The density of the attached micro-colonies increased with an increase in contacting time (from 20, 72 and 96 h) and was indicative of an actively growing mono-layered biofilm.
  • Thumbnail Image
    Item
    Restricted
    Investigation and in situ visualisation of interfacial interactions of thermophilic microorganisms with metal-sulphides in a simulated heap environment
    (Elsevier, 2013) Africa, Cindy-Jade; van Hille, Robert P; Sand, Wolfgang; Harrison, Susan T L
    This study sought to provide a better understanding of the dynamics of microbial-metal sulphide interfacial processes relevant to heap bioleaching. Attachment and subsequent biofilm formation by Metallosphaera hakonensis (M. hakonensis) on the surface of massive chalcopyrite and pyrite samples, as well as a low-grade chalcopyritic whole ore were investigated. The method made use of a biofilm reactor in which thin sections of mineral ore were mounted. Operating conditions in the reactor simulated those of a bioheap in terms of fluid-flow and mineralogy, where the low-grade chalcopyrite ore sections were used. Pure cultures of M. hakonensis were used to inoculate the reactors and the attachment and subsequent biofilm development visualised in situ after 2, 4 and 8 days using a combination of atomic force and epifluorescent microscopy (AFM–EFM) as well as confocal scanning laser microscopy (CSLM). This revealed insights into biofilm structure and architecture. The effect of varying temperature on the extent of attachment and biofilm development was also assessed after 4 days using three temperature regimes: room temperature (20 ± 1 °C), 45 °C and 65 °C. The density of the attached micro–colonies increased with an increase in time, indicative of an actively growing biofilm. The extent of surface coverage and proliferation of the biofilm was dependent on the temperature, with surface coverage being more extensive at 65 °C, near the optimal temperature for growth. Preferential attachment and biofilm formation to sulphide minerals was observed, with increased surface coverage of pyrite mineral surfaces relative to chalcopyrite and low-grade ore. The AFM–EFM technique enhanced the level of detail at which site specific associations of microorganisms with mineral surfaces could be assessed. Spatial orientation and density of attached micro-colonies were noted.
  • Thumbnail Image
    Item
    Open Access
    Investigation of microbial metal-sulfide interfacial environments under mineral bioleach simulated conditions
    (2017) Africa, Cindy-Jade; Harrison, Susan T L; Van Hille, Robert P
    This research pertains to bioleaching of copper containing ores with particular reference to the copper sulfide mineral chalcopyrite (CuFeS2). While it is focused on heap bioleaching, it has applications to stirred tank bioleaching operations. In the context of bioleaching, microbial extra-cellular polymeric substance (EPS) components are thought to complex chemical oxidants and extend the chemical reaction space available for mineral dissolution reactions, making the microbial-mineral-EPS interface the dominant active zone in terms of microbial oxidation and mineral dissolution. There is a limited understanding of microbial biofilm formation within a bioleach heap. The implication of various microorganisms having a set of defined or optimal conditions under which they colonise and proliferate is quite substantial. Understanding what creates favourable interfacial microenvironments enabling a sessile population to flourish (and thereby decrease lag time) has great implications for minimising costs and maximising productivity. Furthermore, limited work has been conducted on thermophilic microorganisms relevant to bioleaching. These microorganisms are pertinent to successful bioleaching at high temperatures, with work incorporating low grade ores and gangue mineralogy also being scarce. The aim of this research is to provide a thorough investigation into microbial-metal sulfide interfacial environments in situ, using a thermophilic archaeon M. hakonensis, low-grade metal-sulfide ores, a series of temperature regimes, heap-simulating conditions and an in depth extraction and analysis of the EPS produced under varied culturing conditions.
  • Thumbnail Image
    Item
    Open Access
    Microbial attachment to sulfide minerals in a bioleach environment
    (2009) Africa, Cindy-Jade; Harrison, STL; Van Hille, Rob
    This research pertains to bioleaching of copper containing ores with particular reference to the copper sulfide mineral chalcopyrite (CuFeS₂). While it is focused on heap bioleaching, it has applications to stirred tank bioleaching operations. Industrial heap bioleaching offers opportunities for processing of low grade ores but poses process operational challenges. These challenges include ineffective heap inoculation, a lag period before effective leaching commences and poor heap performance. These aspects are attributed to several contributing factors, such as heap construction, engineering and microbial activity. To date little attention has been paid to colonisation as a means of mitigating these challenges and effectively improving process operation. Current literature regarding microbial attachment to sulfide minerals is limited to pure culture studies using iron oxidising mesophiles, and the use of sulfide mineral concentrates. In a heap environment, mineral dissolution is accelerated through the presence of a mixed consortium of microbial species; with the contribution of each not yet fully understood. In addition, gangue minerals comprise the bulk of the minerals present and thus cannot be neglected when attempting to better understand microbial attachment and the role of micro-organisms in a heap environment. The predominant methodology employed to study microbial attachment in a bioleach context has used batch agitated systems (shake flasks). This may not adequately represent attachment under heap-like fluid dynamics. The idea of this project stemmed from a requirement to contribute to the mitigation of challenges faced by industry through addressing the aforementioned gaps prevailing in literature and improving understanding of the role of microbial attachment and colonisation under conditions simulating a heap. The aim of this study was to investigate attachment of three bioleach micro-organisms (A. ferrooxidans, L. ferriphilum and S. metallicus)to complex, sulfide-containing minerals ores in a bioleach environment using methodologies simulating heap-like conditions.