Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus

Simple item page
dc.contributor.advisorVon Wechmar, M Barbaraen_ZA
dc.contributor.advisorRybicki, Edward Pen_ZA
dc.contributor.authorBrand, Reon Jen_ZA
dc.date.accessioned2016-10-21T07:36:14Z
dc.date.available2016-10-21T07:36:14Z
dc.date.issued1992en_ZA
dc.descriptionBibliography: pages 188-209.en_ZA
dc.description.abstractWoodiness disease caused by virus infection is the most serious virus disease of passionfruit and affects economic production of this crop worldwide. A preliminary survey of diseased Passiflora material collected from various regions in South Africa revealed the presence of at least three different viruses. A diseased P. caerulea rootstock specimen from a woodiness diseased vineyard in Natal was selected as a source for isolation and further characterization of viruses. Two viruses that were present in a mixed infection were isolated and purified from this material: a spherical virus which appeared to be cucumber mosaic virus (CMV) and a filamentous virus which was initially presumed to be an isolate of passionfruit woodiness virus (PWV). The host range, transmission and prevalence of these viruses were studied by employing techniques such as electron microscopy (negative staining and immunosorbent), electroblot immunoassay, double antibody sandwich enzyme-linked imunoassay and nucleic acid hybridization. In transmission studies, the CMV-isolate and the potyvirus were found to be sap, aphid and graft transmissible. Separation of the two viruses was achieved by passage through a selective host range.en_ZA
dc.identifier.apacitationBrand, R. J. (1992). <i>Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/22240en_ZA
dc.identifier.chicagocitationBrand, Reon J. <i>"Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1992. http://hdl.handle.net/11427/22240en_ZA
dc.identifier.citationBrand, R. 1992. Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Brand, Reon J AB - Woodiness disease caused by virus infection is the most serious virus disease of passionfruit and affects economic production of this crop worldwide. A preliminary survey of diseased Passiflora material collected from various regions in South Africa revealed the presence of at least three different viruses. A diseased P. caerulea rootstock specimen from a woodiness diseased vineyard in Natal was selected as a source for isolation and further characterization of viruses. Two viruses that were present in a mixed infection were isolated and purified from this material: a spherical virus which appeared to be cucumber mosaic virus (CMV) and a filamentous virus which was initially presumed to be an isolate of passionfruit woodiness virus (PWV). The host range, transmission and prevalence of these viruses were studied by employing techniques such as electron microscopy (negative staining and immunosorbent), electroblot immunoassay, double antibody sandwich enzyme-linked imunoassay and nucleic acid hybridization. In transmission studies, the CMV-isolate and the potyvirus were found to be sap, aphid and graft transmissible. Separation of the two viruses was achieved by passage through a selective host range. DA - 1992 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1992 T1 - Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus TI - Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus UR - http://hdl.handle.net/11427/22240 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/22240
dc.identifier.vancouvercitationBrand RJ. Viruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirus. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1992 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/22240en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMicrobiologyen_ZA
dc.titleViruses implicated in the woodiness disease of South African passionfruit, and the molecular characterization of a new potyvirusen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
thesis_sci_1992_brand_reon_j_SR.pdf
Size:
7.38 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
PhD / Doctoral