XvGoIS, a galactinol synthase is transcriptionally upregulated under water deficit : the role of raffinosaccharides in abiotic stress tolerance in the resurrection plant Xerophyta viscosa (Baker)

Master Thesis

2005

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University of Cape Town

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Abstract
As part of an ongoing project to genetically manipulate maize for increased abiotic stress tolerance, we have isolated and identified a number of genes from the resurrection plant Xerophyta viscosa (Baker) that are differentially expressed during water deficit. A cDNA was isolated from a library constructed from the leaves of water stressed X viscosa plants, which showed high identity to galactinol synthase (GoIS) enzymes from a variety of plant species. GolS is fundamental to the biosynthesis of raffinose family oligosachharides (RFOs), sucrosyl carbohydrates that are unique to plants and implicated in carbon translocation and abiotic stress tolerance. We subsequently designated this cDNA XvGoIS.
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Includes bibliographical references (leaves 84-102).

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