Polyacrylamide gradient gel electrophoresis for the diagnosis of dysbetalipoproteinaemia (Type III hyperlipidaemia)
Master Thesis
2001
Permanent link to this Item
Authors
Supervisors
Journal Title
Link to Journal
Journal ISSN
Volume Title
Publisher
Publisher
University of Cape Town
Department
Faculty
License
Series
Abstract
Accurate phenotypic diagnosis of this disorder has generally relied on the quantitative analysis of centrifugally isolated VLDL. Analysis of VLDL chemical composition is not widely available due to the expense of acquiring ultracentrifuges and the highly-skilled staff necessary to perform the analyses. Genotypic diagnosis is somewhat more widely available, especially testing for the E2/E2 genotype which is the commonest underlying genotype in dysbetalipoproteinaemia. Testing for the rarer autosomal mutations of ApoE is restricted to a small number of centres only. In this study I will review dysbetalipoproteinaemia briefly and then describe and evaluate GGE as a new diagnostic technique for this disorder.
Description
Includes bibliographical references.
Reference:
Blom, D. 2001. Polyacrylamide gradient gel electrophoresis for the diagnosis of dysbetalipoproteinaemia (Type III hyperlipidaemia). University of Cape Town.