Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris

 

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dc.contributor.advisor Harrison, STL en_ZA
dc.contributor.advisor Minnaar, Sanet en_ZA
dc.contributor.author Bhardwaj, Vinayak en_ZA
dc.date.accessioned 2014-12-26T14:15:27Z
dc.date.available 2014-12-26T14:15:27Z
dc.date.issued 2011 en_ZA
dc.identifier.citation Bhardwaj, V. 2011. Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/10120
dc.description Includes bibliographical references (leaves 98-103). en_ZA
dc.description.abstract The increasing prevalence of diabetes worldwide has greatly increased the demand for insulin, a key type of treatment for many diabetics. For this purpose, the methylotrophic yeast Pichia pastoris has emerged as an additional microbial host for recombinant insulin production. A genetically modified Pichia pastoris MutS strain, engineered to produce the insulin precursor, was used as the experimental system in this study in order to optimise the insulin production process. The experimental system developed in this study employed a two-stage fed-batch feeding strategy in which growth was optimised by feeding glycerol to boost biomass followed by induction of the gene encoding insulin precursor by feeding methanol. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Chemical Engineering en_ZA
dc.title Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris en_ZA
dc.type Thesis / Dissertation en_ZA
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Engineering & the Built Environment en_ZA
dc.publisher.department Department of Chemical Engineering en_ZA
dc.type.qualificationlevel Masters en_ZA
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image


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