Browsing by Author "Claassen-Weitz, Shantelle"

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Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

Author: Claassen-Weitz, Shantelle ; Gardner-Lubbe, Sugnet ; Mwaikono, Kilaza S ; du Toit, Elloise ; Zar, Heather J ; Nicol, Mark P Date: 12 May 2020 Careful consideration of experimental artefacts is required in order to successfully apply high-throughput 16S ribosomal ribonucleic acid (rRNA) gene sequencing technology. Here we introduce experimental design, quality control and “denoising” ... Read more

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